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卵泡抑素样蛋白1(FSTL1)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Follistatin Like Protein 1 (FSTL1)

FRP; FSL1; Follistatin-related protein 1

  • 卵泡抑素样蛋白1(FSTL1)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 卵泡抑素样蛋白1(FSTL1)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 卵泡抑素样蛋白1(FSTL1)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEJ085Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测卵泡抑素样蛋白1(FSTL1),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的卵泡抑素样蛋白1(FSTL1)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 78-93 83
EDTA plasma(n=5) 85-104 92
heparin plasma(n=5) 79-94 82

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的卵泡抑素样蛋白1(FSTL1),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中卵泡抑素样蛋白1(FSTL1)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 85-104% 96-105% 79-95% 87-101%
EDTA plasma(n=5) 79-96% 88-95% 95-103% 78-92%
heparin plasma(n=5) 88-95% 92-101% 90-97% 83-95%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将卵泡抑素样蛋白1(FSTL1)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的卵泡抑素样蛋白1(FSTL1)与连接于固相载体上的抗体结合,然后加入生物素化的卵泡抑素样蛋白1(FSTL1)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的卵泡抑素样蛋白1(FSTL1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPJ085Hu01 卵泡抑素样蛋白1(FSTL1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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MAJ085Hu22 卵泡抑素样蛋白1(FSTL1)单克隆抗体 WB; IHC
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WEJ085Hu 卵泡抑素样蛋白1(FSTL1)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
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参考文献

杂志 参考文献
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Archives of Physiology and Biochemistry Regulation of follistatin-like protein 1 expression and secretion in primary human skeletal muscle cells [Pubmed: 23419164]
Journal of Proteomics Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation [Pubmed:25118038]
PLOS ONE Growth Differentiation Factor-15 (GDF-15) Levels Are Associated with Cardiac and Renal Injury in Patients Undergoing Coronary Artery Bypass Grafting with Cardiopulmonary Bypass [Pubmed:25171167]
J Mol Med (Berl) Growth hormone replacement therapy regulates microRNA-29a and targets involved in insulin resistance [PubMed: 26199111]
PLoS One. Association of Circulating Follistatin-Like 1 Levels with Inflammatory and Oxidative Stress Markers in Healthy Men [pmc:PMC4856269]
JACC: Basic to Translational Science Follistatin like 1 Regulates Hypertrophy in Heart Failure with Preserved Ejection Fraction. [pubmed:27430031]
Sci Rep Follistatin like-1 aggravates silica-induced mouse lung injury [PMC5428474]
International Journal of Clinical and Experimental Pathology Plasma follistatin-like protein 1 is correlated with disease severity in patients with acute pulmonary embolism and predicts short-term mortality [ISSN:1936-2625]
Endocrine Journal High circulating follistatin-like protein 1 as a biomarker of a metabolically unhealthy state [Pubmed: 30745500]
PLoS One Associations among circulating levels of follistatin-like 1, clinical parameters, and cardiovascular events in patients undergoing elective percutaneous … [Pubmed: 31034503]
British Journal of Cancer Improved early detection of ovarian cancer using longitudinal multimarker models [Pubmed: 31937926]
Cell Communication and Signaling Targeting Follistatin like 1 ameliorates liver fibrosis induced by carbon tetrachloride through TGF-β1-miR29a in mice [Pubmed: 32933544]
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY Effects of acute endurance exercise on follistatin-like 1 and apelin in the circulation and metabolic organs in rats [Pubmed: 32412800]
J Cardiol Prognostic impact of transcardiac gradient of follistatin-like 1 reflecting hemodynamics in patients with dilated cardiomyopathy [34334268]
Int Heart J Association between Plasma Follistatin-like Protein 1 Levels and the Presence and Severity of Coronary Artery Disease [34853217]
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