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肾素(REN)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Renin (REN)

angiotensinogenase

  • 肾素(REN)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 肾素(REN)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 肾素(REN)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA889Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测肾素(REN),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的肾素(REN)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 80-88 84
EDTA plasma(n=5) 81-101 97
heparin plasma(n=5) 95-103 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的肾素(REN),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中肾素(REN)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 79-90% 80-104% 86-102% 85-98%
EDTA plasma(n=5) 93-105% 85-101% 90-98% 87-94%
heparin plasma(n=5) 90-103% 90-97% 99-105% 98-105%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将肾素(REN)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的肾素(REN)与连接于固相载体上的抗体结合,然后加入生物素化的肾素(REN)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的肾素(REN)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA889Hu61 肾素(REN)活性蛋白 Cell culture; Activity Assays.
EPA889Hu61 肾素(REN)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA889Hu01 肾素(REN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA889Hu01 肾素(REN)多克隆抗体 WB; IHC; ICC; IP.
MAA889Hu23 肾素(REN)单克隆抗体 WB; IHC; ICC; IP.
MAA889Hu21 肾素(REN)单克隆抗体 WB; IHC; ICC; IP.
MAA889Hu25 肾素(REN)单克隆抗体 WB; IHC; ICC; IP.
MAA889Hu24 肾素(REN)单克隆抗体 WB; IHC; ICC; IP.
MAA889Hu22 肾素(REN)单克隆抗体 WB; IHC; ICC; IP.
SEA889Hu 肾素(REN)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA889Hu 肾素(REN)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA889Hu 肾素(REN)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Hypertension Prenatal overexposure to glucocorticoids programs renal 11β-hydroxysteroid dehydrogenase type 2 expression and salt-sensitive hypertension in the rat [PubMed: 21045727]
The Journal of Physiology Recombinant human erythropoietin in humans down-regulates proximal renal tubular reabsorption and causes a fall in glomerular filtration rate [Physoc: 194241]
The Journal of Physiology Erythropoietin down‐regulates proximal renal tubular reabsorption and causes a fall in glomerular filtration rate in humans [PubMed: 20724370]
Pathobiology Anti-Angiotensin and Hypoglycemic Treatments Suppress Liver Metastasis of Colon Cancer Cells [Karger: 000330169]
Journal of Cardiovascular Pharmacology The Angiotensin Receptor Blocker, Azilsartan Medoxomil (TAK-491), Suppresses Vascular Wall Expression of Plasminogen Activator Inhibitor Type-I Protein Potentially Facilitating the Stabilization of Atherosclerotic Plaques [LWW: 08000]
Kidney International Multiple and large simple renal cysts are associated with prehypertension and hypertension. [Pubmed: 23389415]
Human Physiology Estimation of the parameters of the renin-angiotensin-aldosterone system, water-electrolyte metabolism, and endothelial function in newborns of women with chronic hypertension [Springer:Source]
Journal of Pharmacological Sciences Organ-Specific Activation of the Gastric Branch of the Efferent Vagus Nerve by Ghrelin in Urethane-Anesthetized Rats [Pubmed:24366191]
Liver International VSL#3 probiotic treatment decreases bacterial translocation in rats with carbon tetrachloride-induced cirrhosis [Pubmed:24750552]
Gynecological Endocrinology Immunological and hemostasiological disorders in women with ovarian hyperstimulation syndrome [Pubmed:25200828]
Oxidative Medicine and Cellular Longevity Age-Associated Changes in the Vascular Renin-Angiotensin System in Mice [Pubmed:27200147]
PLoS One Ablation of Potassium-Chloride Cotransporter Type 3 (Kcc3) in Mouse Causes Multiple Cardiovascular Defects and Isosmotic Polyuria [Pubmed:27166674]
PLOS ONE Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated withActivation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension. [pubmed:27391973]
Heart Lung Circ. Efficacy and Safety of Renal Sympathetic Denervation on Dogs with Pressure Overload-Induced Heart Failure. [pubmed:27555054]
European Journal of Pharmaceutical Sciences Sacubitril/valsartan (LCZ696) Significantly Reduces Aldosterone and Increases cGMP Circulating Levels in a Canine Model of RAAS Activation [Doi: 10.1101/435560]
Nutrition Gut Dysbiosis Contribute to High Fructose Induced Salt Sensitive Hypertension In Sprague-Dawley Rats []
ACTA PHARMACOLOGICA SINICA The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice [33303990]
Hypertens Res Association of Ang-(1¨C7) and des-Arg 9 BK as new biomarkers of obesity and cardiometabolic risk factors in adolescents [33568792]
Phosphorylation of β1-integrin in juxtaglomerular cells helps control blood pressure during the progression of diabetic nephropathy []
Bedeutung des intrarenalen Renin-Angiotensin-Systems f¨¹r die Entwicklung der chronischen Nierenkrankheit []
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