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S100钙结合蛋白A9(S100A9)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for S100 Calcium Binding Protein A9 (S100A9)

MRP14; MIF; 60B8AG; CFAG; NIF; CAGB; CGLB; L1AG; P14; Calgranulin B; Migration inhibitory factor-related protein 14; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain

  • S100钙结合蛋白A9(S100A9)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • S100钙结合蛋白A9(S100A9)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • S100钙结合蛋白A9(S100A9)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEB793Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测S100钙结合蛋白A9(S100A9),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的S100钙结合蛋白A9(S100A9)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 91-105 94
EDTA plasma(n=5) 80-90 81
heparin plasma(n=5) 89-97 93

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的S100钙结合蛋白A9(S100A9),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中S100钙结合蛋白A9(S100A9)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 95-103% 93-105% 78-98% 80-96%
EDTA plasma(n=5) 85-102% 81-88% 95-104% 85-104%
heparin plasma(n=5) 86-101% 91-105% 78-92% 97-104%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将S100钙结合蛋白A9(S100A9)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的S100钙结合蛋白A9(S100A9)与连接于固相载体上的抗体结合,然后加入生物素化的S100钙结合蛋白A9(S100A9)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的S100钙结合蛋白A9(S100A9)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APB793Hu01 S100钙结合蛋白A9(S100A9)活性蛋白 Cell culture; Activity Assays.
RPB793Hu01 S100钙结合蛋白A9(S100A9)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB793Hu01 S100钙结合蛋白A9(S100A9)多克隆抗体 WB; IHC; ICC; IP.
LAB793Hu81 S100钙结合蛋白A9(S100A9)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAB793Hu71 S100钙结合蛋白A9(S100A9)多克隆抗体(生物素标记) WB; IHC; ICC.
MAB793Hu29 S100钙结合蛋白A9(S100A9)单克隆抗体 WB; IHC; ICC; IP.
MAB793Hu22 S100钙结合蛋白A9(S100A9)单克隆抗体 WB; IHC
MAB793Hu21 S100钙结合蛋白A9(S100A9)单克隆抗体 WB; IHC
MAB793Hu23 S100钙结合蛋白A9(S100A9)单克隆抗体 WB
MAB793Hu27 S100钙结合蛋白A9(S100A9)单克隆抗体 WB; IHC; ICC; IP.
MAB793Hu24 S100钙结合蛋白A9(S100A9)单克隆抗体 WB; IHC
MAB793Hu25 S100钙结合蛋白A9(S100A9)单克隆抗体 WB; IHC; ICC; IP.
LAB793Hu82 S100钙结合蛋白A9(S100A9)单克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAB793Hu72 S100钙结合蛋白A9(S100A9)单克隆抗体(生物素标记) WB; IHC; ICC.
SEB793Hu S100钙结合蛋白A9(S100A9)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB793Hu S100钙结合蛋白A9(S100A9)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB793Hu01 S100钙结合蛋白A9(S100A9)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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PLoS One. Proteins with Altered Levels in Plasma from Glioblastoma Patients as Revealed by iTRAQ-Based Quantitative Proteomic Analysis [PubMed: 23029420]
Mainz Identifizierung neuer potentieller Biomarker für das Kolonkarzinom sowie dessen Vorstufen [Info:Source]
PLOS ONE Susceptibility to COPD: Differential Proteomic Profiling after Acute Smoking [Pubmed:25036363]
the faseb journal Soluble receptor for advanced glycation end products inhibits disease progression in autosomal dominant polycystic kidney disease by down-regulating cell proliferation [PubMed: 25934702]
Sci Rep.  S100A9 and ORM1 serve as predictors of therapeutic response and prognostic factors in advanced extranodal NK/T cell lymphoma patients treated with pegaspargase/gemcitabine [pmc:PMC4810364]
Journal of Alzheimer's Disease Proteomics Analysis of Blood Serums from Alzheimer's Disease Patients Using iTRAQ LabelingTechnology. [pubmed:27911324]
International journal of molecular sciences Immune Modulating Topical S100A8/A9 Inhibits Growth of Pseudomonas aeruginosa and Mitigates Biofilm Infection in Chronic Wounds. [pubmed:28672877]
Gastroenterology Tu1938-Micronutrients Assessment in Patients with Cirrhosis [10.1016:S0016-5085(18)33544-3]
Gastroenterology Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis [10.1016:S0016-5085(18)33544-5]
Journal of Biological Chemistry Signaling between pancreatic β-cells and macrophages via S100 calcium-binding protein A8 exacerbates β-cell apoptosis and islet inflammation [Pubmed:29496993]
Cancer Management and Research The role of calgranulin B gene on the biological behavior of squamous cervical cancer in vitro and in vivo [Pubmed:29497331]
European review for medical and pharmacological sciences S100A8/A9 promotes MMP-9 expression in the fibroblasts from cardiac rupture after myocardial infarction by inducing macrophages secreting TNFα. [Pubmed:29949169]
Estudio de células inmunosupresoras y biomarcadores circulantes en carcinoma renal []
Lung Mass Spectrometry Analysis of the Exhaled Breath Condensate and Proposal of Dermcidin and S100A9 as Possible Markers for Lung Cancer Prognosis [Pubmed: 31115649]
中国医药导报 降钙素原, 超敏C 反应蛋白, 红细胞沉降率及S100A9 对肺结核合并肺部细菌感染的影响 []
scandinavian journal of clinical & laboratory investigation Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study [Pubmed: 31856598]
PLoS One Adverse reproductive effects of S100A9 on bovine sperm and early embryonic development in vitro [Pubmed: 31945120]
Sci Rep Overexpression of S100A9 in tumor stroma contribute to immune evasion of NK/T cell lymphoma and predict poor response rate [34045609]
university of copenhagen Adjunctive S100A8/A9 Immunomodulation Hinders Cipro fl oxacin Resistance in []
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