太阳集团tyc5997_平台(china)官方网站

胱天蛋白酶8(CASP8)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Caspase 8 (CASP8)

CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog

  • 胱天蛋白酶8(CASP8)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 胱天蛋白酶8(CASP8)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 胱天蛋白酶8(CASP8)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA853Mu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测胱天蛋白酶8(CASP8),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将胱天蛋白酶8(CASP8)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的胱天蛋白酶8(CASP8)与连接于固相载体上的抗体结合,然后加入生物素化的胱天蛋白酶8(CASP8)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的胱天蛋白酶8(CASP8)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA853Mu01 胱天蛋白酶8(CASP8)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA853Mu02 胱天蛋白酶8(CASP8)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA853Mu01 胱天蛋白酶8(CASP8)多克隆抗体 WB; IHC; ICC; IP.
PAA853Mu02 胱天蛋白酶8(CASP8)多克隆抗体 WB; IHC; ICC; IP.
LAA853Mu81 胱天蛋白酶8(CASP8)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA853Mu71 胱天蛋白酶8(CASP8)多克隆抗体(生物素标记) WB; IHC; ICC.
SEA853Mu 胱天蛋白酶8(CASP8)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA853Mu 胱天蛋白酶8(CASP8)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA853Mu01 胱天蛋白酶8(CASP8)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Molecular Neurobiology Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial Perturbations [Pubmed:25502462]
Journal of Ethnopharmacology Viscum articulatum Burm. f. aqueous extract exerts antiproliferative effect and induces cell cycle arrest and apoptosis in leukemia cells [Pubmed:29555410]
Clinical And Experimental Pharmacology And Physiology  Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway [Pubmed:29266336]
Environmental Toxicology Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino mice [Pubmed: 31087429]
Environ Toxicol Pharmacol. Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in rats [Pubmed: 31421311]
Journal of Biochemical and Molecular Toxicology Tranilast attenuates methotrexate‐induced renal and hepatic toxicities: Role of apoptosis‐induced tissue proliferation [Pubmed: 32045101]
life sciences Nifuroxazide attenuates experimentally-induced hepatic encephalopathy and the associated hyperammonemia and cJNk/caspase-8/TRAIL activation in rats [Pubmed: 32259601]
Odontology Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells [Pubmed: 33206337]
Int Immunopharmacol Cardio-protective impact of gabapentin against doxorubicin-induced myocardial toxicity in rats; emphasis on modulation of inflammatory-apoptotic signaling [Pubmed: 33199237]
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY Chemo-preventive effect of crocin against experimentally-induced hepatocarcinogenesis via regulation of apoptotic and Nrf2 signaling pathways [Pubmed: 32942000]
Environ Sci Pollut Res Int Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low− dose of … [Pubmed: 32915453]
J Biochem Mol Toxicol Tranilast abrogates cisplatin©\induced testicular and epididymal injuries: An insight into its modulatory impact on apoptosis/proliferation [34047436]
留言咨询
Baidu
sogou