太阳集团tyc5997_平台(china)官方网站

表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Epidermal Growth Factor (EGF)

URG; Beta-Urogastrone; Pro-epidermal growth factor

  • 表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA560Mu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测表皮生长因子(EGF),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的表皮生长因子(EGF)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 89-97 93
EDTA plasma(n=5) 85-98 92
heparin plasma(n=5) 78-90 87

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的表皮生长因子(EGF),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中表皮生长因子(EGF)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 93-102% 85-97% 98-105% 97-105%
EDTA plasma(n=5) 78-94% 96-104% 98-105% 78-92%
heparin plasma(n=5) 86-96% 85-101% 92-101% 88-96%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将表皮生长因子(EGF)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的表皮生长因子(EGF)与连接于固相载体上的抗体结合,然后加入生物素化的表皮生长因子(EGF)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的表皮生长因子(EGF)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA560Mu02 表皮生长因子(EGF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA560Mu01 表皮生长因子(EGF)活性蛋白 Cell culture; Activity Assays.
RPA560Mu01 表皮生长因子(EGF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA560Mu02 表皮生长因子(EGF)多克隆抗体 WB; IHC; ICC; IP.
PAA560Mu01 表皮生长因子(EGF)多克隆抗体 WB; IHC; ICC; IP.
SEA560Mu 表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA560Mu 表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA560Mu 表皮生长因子(EGF)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA560Mu 表皮生长因子(EGF)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Journal of Pediatric Surgery The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model [PubMed: 20438940]
Biomark Insights Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism [PubMed: PMC3623607]
Plos one The Probiotic Mixture VSL#3 Accelerates Gastric Ulcer Healing by Stimulating Vascular Endothelial Growth Factor [PubMed: PMC3590171]
Diabetes. Allogeneic transplantation of an adipose-derived stem cell (ASC) sheet combined with artificial skin accelerates wound healing in a rat wound model of type 2 diabetes and obesity [Pubmed:25795216]
Eur Rev Med Pharmacol Sci.? Association of colon adenomas and skin tags: coincidence or coexistence? [Pubmed:24763889]
Int J Clin Exp Med. The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell [PubMed: 26628972]
ACS Applied Materials & Interfaces Polydopamine nanoparticles modulating stimuli-responsive PNIPAM hydrogels with cell/tissue adhesiveness. [pubmed:27709887]
Canadian Journal of Physiology and Pharmacology The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats [pubmed:28177680]
Journal of Controlled Release A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injury [pubmed:28522195]
Scientific Reports Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets [pubmed:28811665]
International Journal of Nanomedicine Micro-/nano-topography of selective laser melting titanium enhances adhesion and proliferation and regulates adhesion-related gene expressions of human … [Pubmed: 30233172]
Neoplasia Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicine [Pubmed: 33212364]
Aesthet Surg J Adipose-Derived Stem Cells Improve the Aging Skin of Nude Mice by Promoting Angiogenesis and Reducing Local Tissue Water [33428732]
Cell Death & Disease Sodium/glucose cotransporter 1-dependent metabolic alterations induce tamoxifen resistance in breast cancer by promoting macrophage M2 polarization [34006822]
Stem Cells International The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin?¡­ [33381190]
Molecular Biology Reports Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … [Pubmed:35585377]
Biomaterials Advances Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats []
Authorea Syringaresinol attenuates kidney injury by inhibition of pyroptosis via activating the Nrf2 antioxidant pathway in diabetic nephropathy []
Biointerface Research in Applied Chemistry Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats []
留言咨询
Baidu
sogou