肝细胞生长因子(HGF)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Hepatocyte Growth Factor (HGF)
HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen
- 编号SEA047Mu
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围15.6-1000pg/mL
- 灵敏度最小可检测剂量小于等于5.5pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2722 ¥ 3888 ¥ 17496 ¥ 33048 ¥ 272160
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测肝细胞生长因子(HGF)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的肝细胞生长因子(HGF)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 89-103 | 96 |
| EDTA plasma(n=5) | 78-95 | 87 |
| heparin plasma(n=5) | 91-99 | 96 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的肝细胞生长因子(HGF)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中肝细胞生长因子(HGF)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-101% | 88-102% | 83-101% | 95-102% |
| EDTA plasma(n=5) | 89-104% | 91-105% | 81-90% | 90-99% |
| heparin plasma(n=5) | 78-89% | 78-90% | 90-98% | 97-105% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将肝细胞生长因子(HGF)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的肝细胞生长因子(HGF)与连接于固相载体上的抗体结合，然后加入生物素化的肝细胞生长因子(HGF)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的肝细胞生长因子(HGF)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA047Mu02 | 肝细胞生长因子(HGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA047Mu01 | 肝细胞生长因子(HGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA047Mu01 | 肝细胞生长因子(HGF)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA047Mu02 | 肝细胞生长因子(HGF)多克隆抗体 | WB; IHC; ICC; IP. |
| SEA047Mu | 肝细胞生长因子(HGF)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA047Mu | 肝细胞生长因子(HGF)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA047Mu | 肝细胞生长因子(HGF)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
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| Journal of Translational Medicine | Low temperature of radiofrequency ablation at the target sites can facilitate rapid progression of residual hepatic VX2 carcinoma [BioMed: 14795876] |
| Digestive Diseases and Sciences | Gene Transfer of c-met Confers Protection Against d-Galactosamine/Lipopolysaccharide-Induced Acute Liver Failure [SpringerLink: k22823l138x2344x] |
| Biomarker insights | Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children [PubMed: PMC3694825] |
| Biomarker insights | Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) [PubMed: PMC3762604] |
| BMC cell biology | BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause [PubMed: PMC3640998] |
| Cell Journal (Yakhteh) | Effect of Mesenchymal Stem Cells on Doxorubicin-Induced Fibrosis [PubMed: PMC3584430] |
| Gastroenterol Res Pract | Proinflammatory Cytokines (IL-1α, IL-6) and Hepatocyte Growth Factor in Patients with Alcoholic Liver Cirrhosis [PubMed: 26448742] |
| Tumor Biology | Hypoxia-induced secretion of IL-11 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151] |
| Cytotherapy | Cell type–dependent variation in paracrine potency determines therapeutic efficacy against neonatal hyperoxic lung injury [PubMed: 25863963] |
| Biomedicine & Pharmacotherapy | Silymarin-loaded Eudragit® RS100 nanoparticles improved the ability of silymarin to resolve hepatic fibrosis in bile duct ligated rats [Pubmed:27261582] |
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| Changes in HGF levels after a progressive test before and after Tabata HIIT program [profile:Pawel_Wolanski3] | |
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| Stem Cell Research & Therapy | Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure. [pubmed:28679425] |
| Nature Communications | Pulmonary pericytes regulate lung morphogenesis [Pubmed:29934496] |
| Experimental And Molecular Medicine | Interleukin-1 receptor antagonist-mediated neuroprotection by umbilical cord-derived mesenchymal stromal cells following transplantation into a rodent stroke … [Pubmed:29650950] |
| Oncology Letters | Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitro [Pubmed:29375714] |
| Bulletin of Experimental Biology and Medicine | Dynamics of Expression of Cytokine Genes and Macrophage Content in the Lungs and Kidneys after Subtotal Hepatectomy in Rats [Pubmed:29796817] |
| Cell Biology International | Inherent control of hepatocyte proliferationInherent control of hepatocyte proliferation after subtotal liver resection [Pubmed: 31297922] |
| Journal of stem cells & regenerative medicine | What is the Best Degree of Hyaluronic Acid Crosslinking in Increasing Growth Factors Level of Platelet-Rich Fibrin Lysate? [Pubmed: 31239605] |
| Open Journal of Veterinary Medicine | Efficacy of autologous bone marrow mononuclear cell transplantation in dogs with chronic spinal cord injury [Pubmed: 32821665] |
| Stem Cell Reviews and Reports | Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care [33469783] |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … [Pubmed:35585377] |
