太阳集团tyc5997_平台(china)官方网站

环加氧酶2(COX 2)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Cyclooxygenase-2 (COX 2)

PGG/HS; PGHS2; PHS2; HCox2; PTGS2; Prostaglandin Endoperoxide Synthase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2

  • 环加氧酶2(COX 2)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 环加氧酶2(COX 2)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 环加氧酶2(COX 2)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA699Mu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测环加氧酶2(COX 2)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的环加氧酶2(COX 2)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 90-99 96
EDTA plasma(n=5) 78-89 81
heparin plasma(n=5) 85-102 91

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的环加氧酶2(COX 2)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中环加氧酶2(COX 2)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 87-101% 82-94% 81-98% 91-105%
EDTA plasma(n=5) 91-105% 99-105% 85-101% 79-97%
heparin plasma(n=5) 84-105% 95-103% 86-105% 93-105%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将环加氧酶2(COX 2)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的环加氧酶2(COX 2)与连接于固相载体上的抗体结合，然后加入生物素化的环加氧酶2(COX 2)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的环加氧酶2(COX 2)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPA699Mu02 环加氧酶2(COX 2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA699Mu01 环加氧酶2(COX 2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA699Mu01 环加氧酶2(COX 2)多克隆抗体 WB; IHC; ICC; IP.
PAA699Mu02 环加氧酶2(COX 2)多克隆抗体 WB; IHC; ICC; IP.
LAA699Mu81 环加氧酶2(COX 2)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA699Mu71 环加氧酶2(COX 2)多克隆抗体(生物素标记) WB; IHC; ICC.
SEA699Mu 环加氧酶2(COX 2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA699Mu 环加氧酶2(COX 2)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Photodermatology, Photoimmunology & Photomedicine Diallyl sulfide protects against ultraviolet B-induced skin cancers in SKH-1 hairless mouse: analysis of early molecular events in carcinogenesis [Wiley: source]
Skin Cancer - MedlinePlus Health Information Molecular mechanisms underlying chemopreventive activities of glycyrrhizic acid against UVB-radiation-induced carcinogenesis in SKH-1 hairless mouse epidermis [PubMed: 21545294]
Evidence-Based Complementary and Alternative Medicine Curcumin Protects against UVB-Induced Skin Cancers in SKH-1 Hairless Mouse: Analysis of Early Molecular Markers in Carcinogenesis [PubMed: 22888366]
Integrative Cancer Therapies Decalepis hamiltonii inhibits tumor progression and metastasis by regulating the inflammatory mediators and nuclear factor κB subunits [Pubmed: 24013642]
Journal of ethnopharmacology Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats. [Pubmed: 24211397]
Int J Clin Exp Pathol Relationship between epidermal growth factor receptor (EGFR) mutation and serum cyclooxygenase-2 Level, and the synergistic effect of celecoxib and gefitinib on EGFR expression in non-small cell lung cancer cells [PubMed: 26464643]
Int J Clin Exp Pathol Monitoring of cyclooxygenase-2 levels can predict EGFR mutations and the efficacy of EGFR-TKI in patients with lung adenocarcinoma [PubMed: 26191267]
Journal of Biomechanics The shear wave elastic modulus and the increased nuclear factor kappa B(NF-kB/p65) and cyclooxygenase-2 (COX-2) expression in the area of myofascial trigger points activated in a rat model by blunt trauma to the vastus medialis [pubmed:29137729]
Journal of Global Pharma Technology Inflammatory Response of Annona Muricata Linn Leaves Extract in Colorectal Cancer Patients [NCT02439580]
International Journal of Molecular Sciences High Expression of IL-1RI and EP2 Receptors in the IL-1β/COX-2 Pathway, and a New Alternative to Non-Steroidal Drugs—Osthole in Inhibition COX-2 [Pubmed: 30620999]
International Immunopharmacology A novel concept of immunological and allergy interactions in Autism Spectrum Disorders: Molecular, anti-inflammatory effect of osthole [Pubmed: 30953868]
Reports of Biochemistry & Molecular Biology Evaluation of Serum Cyclooxygenase, Hepcidin Levels in Acute Renal Injury (AKI) Patients Following Cardiac Catheterization []
J Ethnopharmacol Isolation and characterization of anti-inflammatory and anti-proliferative compound, for B-cell Non-Hodgkin lymphoma, from Nyctanthes arbor-tristis Linn. [Pubmed:35398498]
留言咨询
Baidu
sogou