血栓调节蛋白(TM)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Thrombomodulin (TM)
CD141; THBD; BDCA3; THRM; Fetomodulin
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测血栓调节蛋白(TM)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的血栓调节蛋白(TM)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| sodium citrate plasma(n=5) | 91-98 | 94 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的血栓调节蛋白(TM)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血栓调节蛋白(TM)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| sodium citrate plasma(n=5) | 98-105% | 80-99% | 78-98% | 90-99% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将血栓调节蛋白(TM)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的血栓调节蛋白(TM)与连接于固相载体上的抗体结合，然后加入生物素化的血栓调节蛋白(TM)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血栓调节蛋白(TM)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA529Mu02 | 血栓调节蛋白(TM)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA529Mu01 | 血栓调节蛋白(TM)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA529Mu01 | 血栓调节蛋白(TM)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA529Mu02 | 血栓调节蛋白(TM)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA529Mu71 | 血栓调节蛋白(TM)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA529Mu | 血栓调节蛋白(TM)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA529Mu | 血栓调节蛋白(TM)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA529Mu | 血栓调节蛋白(TM)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| PLoS One | A Non-Lethal Traumatic/Hemorrhagic Insult Strongly Modulates the Compartment-Specific PAI-1 Response in the Subsequent Polymicrobial Sepsis [PubMed: PMC3568129] |
| Clin Appl Thromb Hemost. | Is There Any Association Between Thrombosis and Tissue Factor Pathway Inhibitor Levels in Patients With Vitamin D Deficiency? [Pubmed: 24203351] |
| медицинские науки | КЛЕТОЧНО-МОЛЕКУЛЯРНЫЕ МАРКЕРЫ ЭНДОТЕЛИАЛЬНОЙ ДИСФУНКЦИИ И СИСТЕМНОГО ГЕМОСТАЗА ПРИ ВИБРАЦИОННОЙ БОЛЕЗНИ В … [Ru:Source] |
| FASEB J. | Endothelial mineralocorticoid receptor activation enhances endothelial protein C receptor and decreases vascular thrombosis in mice [Pubmed:24451386] |
| Gynecol Endocrinol. | The effect of hormone therapy on biochemical and ultrasound parameters associated with atherosclerosis in 46, XY DSD individuals with female phenotype [Pubmed:24911331] |
| Informa Healthcare | Changes in cardiac thrombomodulin and heat shock transcription factor 1 expression and peripheral thrombomodulin and catecholamines during hypothermia in rats [Pubmed:25109347] |
| Bone Marrow Transplant. | Thrombomodulin alleviates murine GVHD in association with an increase in the proportion of regulatory T cells in the spleen [Pubmed:25243628] |
| Biomed Res Int | Basic Fibroblast Growth Factor Ameliorates Endothelial Dysfunction in Radiation-Induced Bladder Injury [PubMed: 26351640] |
| Molecular Nutrition & Food Research | Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors [PubMed: 25546502] |
| Acta Biomater | Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture study [PubMed: 26689470] |
| MEDICINE AND EDUCATION IN SIBERIA | КЛЕТОЧНО-МОЛЕКУЛЯРНЫЕ МАРКЕРЫ ЭНДОТЕЛИАЛЬНОЙ ДИСФУНКЦИИ И СИСТЕМНОГО ГЕМОСТАЗА ПРИ ВИБРАЦИОННОЙ БОЛЕЗНИ В СОЧЕТАНИИ С АРТЕРИАЛЬНОЙ ГИПЕРТЕНЗИЕЙ [Cozo: Mos] |
| Acta Biomaterialia | Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture study [Pubmed:26689470] |
| Bone Marrow Transplantation | The fifth epidermal growth factor-like region of thrombomodulin exerts cytoprotective functionand prevents SOS in a murine model. [pubmed:27427915] |
| BMC Vet Res. | Blood markers of fibrinolysis and endothelial activation in canine babesiosis. [pubmed:28363279] |
| Haematologica | Cytoprotective and pro-angiogenic functions of thrombomodulin are preserved in the C loop of the fifth epidermal growth factor-like domain [Pubmed:29903766] |
| Biomedicine & Pharmacotherapy | Puerarin prevents vascular endothelial injury through suppression of NF-κB activation in LPS-challenged human umbilical vein endothelial cells [Pubmed:29775893] |
| Scientific Reports | Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea [Pubmed: 31882827] |
| Intensive Care Medicine Experimental | Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental … [34169407] |
