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红细胞衍生核因子2样蛋白2(NFE2L2)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2)

NRF2; HEBP1; NF-E2-Related Factor 2; Nuclear factor, erythroid derived 2, like 2

  • 红细胞衍生核因子2样蛋白2(NFE2L2)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 红细胞衍生核因子2样蛋白2(NFE2L2)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 红细胞衍生核因子2样蛋白2(NFE2L2)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEL947Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测红细胞衍生核因子2样蛋白2(NFE2L2)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将红细胞衍生核因子2样蛋白2(NFE2L2)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的红细胞衍生核因子2样蛋白2(NFE2L2)与连接于固相载体上的抗体结合，然后加入生物素化的红细胞衍生核因子2样蛋白2(NFE2L2)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的红细胞衍生核因子2样蛋白2(NFE2L2)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPL947Hu01 红细胞衍生核因子2样蛋白2(NFE2L2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAL947Hu01 红细胞衍生核因子2样蛋白2(NFE2L2)多克隆抗体 WB; IHC; ICC; IP.
MAL947Hu21 红细胞衍生核因子2样蛋白2(NFE2L2)单克隆抗体 WB; IHC; ICC; IP.
SEL947Hu 红细胞衍生核因子2样蛋白2(NFE2L2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LML947Hu 红细胞衍生核因子2样蛋白2(NFE2L2)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Peptides. Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms. [pubmed:27777064]
european journal of pharmacology Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: Possible involved mechanisms. [pubmed:27915041]
european journal of pharmacology S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase. [pubmed:27887948]
Psychopharmacology (Berl) Ellagic acid ameliorates learning and memory deficits in a rat model of Alzheimer's disease: an exploration of underlying mechanisms. [pubmed:28303372]
brain research Ellagic acid exerts protective effect in intrastriatal 6-hydroxydopamine rat model of Parkinson's disease: Possible involvement of ERβ/Nrf2/HO-1 signaling. [pubmed:28238669]
Inflammopharmacology. Implication of Nrf2/HO-1 pathway in the coloprotective effect of coenzyme Q10 against experimentally induced ulcerative colitis. [pubmed:28050757]
Biomedicine & Pharmacotherapy Ameliorative potential of sitagliptin and/or resveratrol on experimentallyinduced clear cell renal cell carcinoma [pubmed:29101811]
Asian Pacific Journal of Cancer Prevention Ameliorative Effect of Coenzyme Q10 and/or Candesartan on Carboplatin-Induced Nephrotoxicity: Roles of Apoptosis, Transforming Growth Factor-Β1, Nuclear Factor Kappa-B And The Nrf2/HO-1 Pathway [pubmed:28670881]
Nutrients Effect of Green and Brown Propolis Extracts on the Expression Levels of microRNAs, mRNAs and Proteins, Related to Oxidative Stress and Inflammation. [pubmed:28974022]
Cytokine Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects [pubmed:29102164]
Biomedicine & pharmacotherapy Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms [pubmed:28061403]
International Immunopharmacology Scutellarin alleviates lipopolysaccharide-induced cognitive deficits in the rat: Insights into underlying mechanisms. [pubmed:29190543]
Neurotoxicology Molecular mechanisms of neuroprotective effect of adjuvant therapy with phenytoin in pentylenetetrazole-induced seizures: impact on Sirt1/NRF2 signaling pathways [Pubmed:30017425]
Biomedicine & Pharmacotherapy Targeting Nrf2/HO-1 signaling by crocin: Role in attenuation of AA-induced ulcerative colitis in rats [Pubmed: 30530041]
Toxicology Reports IL-6 and NFE2L2: A putative role for the hepatoprotective effect of N. Sativa, P. Ginseng and C. Sempervirens in AFB-1 induced hepatocellular carcinoma in … []
Redox Biology Short-term pharmacological activation of Nrf2 ameliorates vascular dysfunction in aged rats and in pathological human vasculature. A potential target for … [Pubmed: 31302408]
Life Sciences Crocin attenuates lung inflammation and pulmonary vascular dysfunction in a rat model of bleomycin-induced pulmonary fibrosis [Pubmed: 31465731]
Respiratory physiology & neurobiology Targeting oxidative stress, proinflammatory cytokines, apoptosis and toll like receptor 4 by empagliflozin to ameliorate bleomycin-induced lung fibrosis [Pubmed: 31600583]
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY Effects of repeated increasing doses of cisplatin as models of acute kidney injury and chronic kidney disease in rats [Pubmed: 32936352]
Iranian Journal of Medical Sciences Esculetin Alleviates Acute Liver Failure following Lipopolysaccharide/D-Galactosamine in Male C57BL/6 Mice []
Can J Physiol Pharmacol Taurine alleviates kidney injury in a thioacetamide rat model by mediating Nrf2/HO-1, NQO-1 and MAPK/NF-κB signaling pathways [34695366]
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