骨膜蛋白(POSTN)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Periostin (POSTN)
OSF-2; PDLPOSTN; PN; Osteoblast Specific Factor 2
- 编号SEH339Mu
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.156-10ng/mL
- 灵敏度最小可检测剂量小于等于0.055ng/mL.
- 样本类型serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3024 ¥ 4320 ¥ 19440 ¥ 36720 ¥ 302400
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测骨膜蛋白(POSTN)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的骨膜蛋白(POSTN)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 91-101 | 95 |
| EDTA plasma(n=5) | 91-101 | 96 |
| heparin plasma(n=5) | 84-99 | 90 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的骨膜蛋白(POSTN)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中骨膜蛋白(POSTN)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-102% | 88-96% | 80-105% | 83-101% |
| EDTA plasma(n=5) | 87-97% | 78-103% | 89-97% | 91-98% |
| heparin plasma(n=5) | 94-102% | 79-101% | 86-94% | 88-98% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将骨膜蛋白(POSTN)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的骨膜蛋白(POSTN)与连接于固相载体上的抗体结合，然后加入生物素化的骨膜蛋白(POSTN)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的骨膜蛋白(POSTN)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPH339Mu01 | 骨膜蛋白(POSTN)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPH339Mu02 | 骨膜蛋白(POSTN)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAH339Mu02 | 骨膜蛋白(POSTN)多克隆抗体 | WB; IHC; ICC; IP. |
| PAH339Mu03 | 骨膜蛋白(POSTN)多克隆抗体 | WB; IHC; ICC; IP. |
| PAH339Mu01 | 骨膜蛋白(POSTN)多克隆抗体 | WB; IHC; ICC; IP. |
| LAH339Mu71 | 骨膜蛋白(POSTN)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAH339Mu81 | 骨膜蛋白(POSTN)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| SEH339Mu | 骨膜蛋白(POSTN)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMH339Mu | 骨膜蛋白(POSTN)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Blood | Circulating Periostin Is Elevated In Patients With Hemoglobinopathies and Correlates With Bone Mineral Density In Double Heterozygous Sickle-Cell/Beta-Thalassemia Patients; A Novel Marker Of Bone Strength? [Bloodjournal: Source] |
| The Journal of Clinical Endocrinology & Metabolism | Serum periostin is associated with fracture risk in postmenopausal women: a 7 years prospective analysis of the OFELY study [Pubmed: 24628551] |
| J Clin Endocrinol Metab. | Serum Periostin Is Associated With Fracture Risk in Postmenopausal Women: A 7-Year Prospective Analysis of the OFELY Study [Pubmed:24628551] |
| Rheumatology (Oxford). | Circulating periostin levels in patients with AS: association with clinical and radiographic variables, inflammatory markers and molecules involved in bone formation [Pubmed:25349442] |
| Journal of Periodontal Research | Assessment of periostin levels in serum and gingival crevicular fluid of patients with periodontal disease [Pubmed:25529858] |
| 新医学 | 血清内脂素水平与多囊卵巢综合征的相关性研究 [Xinyixue:Source] |
| Osteoarthritis | Serum periostin is associated with prevalent knee osteoarthritis and disease incidence/progression in women: the OFELY study [PubMed: 26072384] |
| Endocrine | Serum periostin is a potential biomarker for non-alcoholic fatty liver disease: a case– control study [PubMed: 26362060] |
| Blood Cancer Journal | High levels of periostin correlate with increased fracture rate, diffuse MRI pattern, abnormal bone remodeling and advanced disease stage in patients with newly … [pubmed:27716740] |
| Metabolism. | Low periostin levels in adult patients with Langerhans cell histiocytosis are independently associated with the disease activity. [pubmed:28521873] |
| Osteoporosis International | Circulating periostin levels increase in association with bone density loss and healing progression during the early phase of hip fracture in Chinese older women [pubmed:28382553] |
| Molecular Diagnosis & Therapy | The Utility of Biomarkers in Osteoporosis Management [pubmed:28271451] |
| Journal of Bone and Mineral Research | Cortical and trabecular bone microstructure did not recover at weight-bearing skeletal sites and progressively deteriorated at non-weight-bearing sites during the year following International Space Station missions [doi:10.1002] |
| Clinical Cases in Mineral and Bone Metabolism | Periostin and sclerostin levels in juvenile Paget's disease [pubmed:29263750] |
| Calcified Tissue International | Development of a New Immunoassay for Human Cathepsin K-Generated Periostin Fragments as a Serum Biomarker for Cortical Bone [pubmed:28725907] |
| Wiley | Characterization of a sandwich ELISA for the quantification of all human periostin isoforms [pubmed:28493527] |
| International Osteoporosis Foundation and National Osteoporosis Foundation | Interaction between LRP5 and periostin gene polymorphisms on serum periostin levels and cortical bone microstructure [pubmed:29038835] |
| Bone | Effect of age and gender on serum periostin: Relationship to cortical measures, bone turnover and hormones [pubmed:28323143] |
| Clinica Chimica Acta | Association between serum periostin concentrations and outcome after acute spontaneous intracerebral hemorrhage [pubmed:28882488] |
| Clinica Chimica Acta | Serum periostin concentrations and outcomes after severe traumatic brain injury [pubmed:28668564] |
| Tumor Biology | Predictive and prognostic value of serum periostin in advanced non–small cell lung cancer patients receiving chemotherapy [pubmed:28459197] |
| Journal of Bone and Mineral Research | Serum Levels of a Cathepsin-K Generated Periostin Fragment Predict Incident Low-Trauma Fractures in Postmenopausal Women Independently of BMD and FRAX [pubmed:28766739] |
| ОСОБЕННОСТИ СОСТОЯНИЯ ЗДОРОВЬЯ НОВОРОЖДЕННЫХ, РОЖДЕННЫХ ОТ МАТЕРЕЙ С БРОНХИАЛЬНОЙ АСТМОЙ [2017; 62:(4)] | |
| Endocrine | Circulating periostin in patients with nonalcoholic fatty liver disease. [pubmed:27738886] |
| Molecular Medicine Reports | Periostin cross‑reacts with the renin‑angiotensin system during liver fibrosis development [pubmed:28849131] |
| The Journal of Biological Chemistry | Toll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in mice [Pubmed:29929978] |
| Calcified Tissue International | The C-Terminal Intact Forms of Periostin (iPTN) Are Surrogate Markers for Osteolytic Lesions in Experimental Breast Cancer Bone Metastasis [Pubmed:29916127] |
| Journal of Endocrinological Investigation | Increased serum periostin concentrations are associated with the presence of diabetic retinopathy in patients with type 2 diabetes mellitus [Pubmed:29349642] |
| Wiener klinische Wochenschrift | Rheumatoid arthritis in remission [] |
| Clinical and Experimental Medicine | Myostatin and other musculoskeletal markers in lung transplant recipients [Pubmed: 30317402] |
| Experimental and Clinical Endocrinology & Diabetes | Polycystic Ovary Syndrome is Associated with Elevated Periostin Levels [Pubmed: 30372763] |
| ИСПОЛЬЗОВАНИЕ СЫВОРОТОЧНОГО ПЕРИОСТИНА В КАЧЕСТВЕ МАРКЕРА ОБОСТРЕНИЙ АСТМЫ У ДЕТЕЙ [] | |
| Bone | Tartrate-resistant acid phosphatase 5b, but not periostin, is useful for assessing Paget's disease of bone [Pubmed: 31051316] |
| Journal of Clinical Laboratory Analysis | Identification and diagnostic value of pleural fluid periostin and serum periostin of malignant pleural effusions in patients with non–small‐cell lung cancer [Pubmed: 31268191] |
| Bone | Serum periostin is associated with cancer mortality but not cancer risk in older home-dwelling men: A 8-year prospective analysis of the STRAMBO study [Pubmed: 31812700] |
| FASEB J | Fibronectin rescues aberrant phenotype of endothelial cells lacking either CCM1, CCM2 or CCM3 [Pubmed: 32515053] |
| Journal of Reproductive Immunology | Serum and placental periostin levels in women with early pregnancy loss [Pubmed: 32460058] |
| Annals of Medical Research | Evaluation of periostin levels in gingival crevicular fluid and peri-implant sulcus fluid in patients with periodontal and peri-implanter disease: A cross-sectional … [] |
| Myostatin and Markers of Bone Metabolism in Dermatomyositis [] | |
| Am J Physiol Heart Circ Physiol | Periostin promotes arterial calcification through PPAR¦Ã-related glucose metabolism reprogramming [33834866] |
| International Journal of Chronic Obstructive Pulmonary Disease | High Blood Eosinophil and YKL-40 Levels, as Well as Low CXCL9 Levels, are Associated with Increased Readmission in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease [33814903] |
| J Bone Oncol | Serum total periostin is an independent marker of overall survival in bone metastases of lung adenocarcinoma [34150488] |
| Rambam Maimonides Med J | The Relationship between Serum Periostin Levels and Neutrophil¨CLymphocyte Ratio with Lesion Localization and Acute Prognosis in Acute Ischemic Stroke [34137680] |
| Rambam Maimonides Medical Journal | First Admission Neutrophil–Lymphocyte Ratio May Indicate Acute Prognosis of Ischemic Stroke [34137680] |
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