血管内皮生长因子A(VEGFA)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)
VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor
- 编号SEA143Po
- 物种Sus scrofa; Porcine (Pig，猪) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围15.6-1,000pg/mL
- 灵敏度最小可检测剂量小于等于6.4pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3175 ¥ 4536 ¥ 20412 ¥ 38556 ¥ 317520
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测血管内皮生长因子A(VEGFA)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的血管内皮生长因子A(VEGFA)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 78-104 | 101 |
| EDTA plasma(n=5) | 87-96 | 93 |
| heparin plasma(n=5) | 90-98 | 94 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的血管内皮生长因子A(VEGFA)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血管内皮生长因子A(VEGFA)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-91% | 80-96% | 98-105% | 97-104% |
| EDTA plasma(n=5) | 82-98% | 98-105% | 87-101% | 90-98% |
| heparin plasma(n=5) | 94-103% | 88-102% | 84-101% | 83-104% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将血管内皮生长因子A(VEGFA)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的血管内皮生长因子A(VEGFA)与连接于固相载体上的抗体结合，然后加入生物素化的血管内皮生长因子A(VEGFA)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管内皮生长因子A(VEGFA)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Sus scrofa; Porcine (Pig，猪) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA143Po01 | 血管内皮生长因子A(VEGFA)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA143Po02 | 血管内皮生长因子A(VEGFA)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA143Po01 | 血管内皮生长因子A(VEGFA)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA143Po81 | 血管内皮生长因子A(VEGFA)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAA143Po71 | 血管内皮生长因子A(VEGFA)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA143Po21 | 血管内皮生长因子A(VEGFA)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA143Po23 | 血管内皮生长因子A(VEGFA)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA143Po22 | 血管内皮生长因子A(VEGFA)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA143Po | 血管内皮生长因子A(VEGFA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA143Po | 血管内皮生长因子A(VEGFA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
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| Randomized Controlled Trial | Effect of Freeze-Dried Amnion Graft on Cytokines in Uterine Exudates Following Hysteroscopic Adhesiolysis of Severe Intrauterine Adhesions [28170094] |
| cancer communications | Programmed death ligand©\1 regulates angiogenesis and metastasis by participating in the c©\JUN/VEGFR2 signaling axis in ovarian cancer [33939321] |
| Öйú¼ÄÉú³æѧÓë¼ÄÉú³æ²¡ÔÓÖ¾ | VEGFA/VEGFR2 ÔÚСÊó¸Î¶à·¿¼¬ÇòòÊ×é֯Ѫ¹ÜÉú³ÉÖеıí´ï¼°×÷Óà [] |
| Chinese Journal of Parasitology and parasitic diseases | Expression and angiogenic effect of VEGFA/VEGFR2 in mice hepatic metacestode tissue of Echinococcus multilocularis [] |
| International Journal of Life science and Pharma Research | Artificial Sweeteners Alter Early Vitelline Vessel Development in Chick Embryo.(2021) [] |
| Biomed Mater | Preclinical efficacy study of a porous biopolymeric scaffold based on (gelatin-hyaluronic acid-chondroitin sulfate) in a porcine burn injury model: Role of critical … [34384056] |
| J Cell Mol Med | Mesenchymal stem cells from different sources show distinct therapeutic effects in hyperoxia‐induced bronchopulmonary dysplasia in rats [34322990] |
| J Biochem Mol Toxicol | Thymoquinone upregulates miR‐125a‐5p, attenuates STAT3 activation, and potentiates doxorubicin antitumor activity in murine solid Ehrlich carcinoma [34605108] |
| Bioengineered | MicroRNA-934 facilitates cell proliferation, migration, invasion and angiogenesis in colorectal cancer by targeting B-cell translocation gene 2: Running title: miR934 … [34699325] |
| ACS Biomater Sci Eng | Polydopamine-Coated Poly(l-lactide) Nanofibers with Controlled Release of VEGF and BMP-2 as a Regenerative Periosteum [34472855] |
| Cells | A-Kinase Anchoring Protein 2 Promotes Protection against Myocardial Infarction [34831084] |
| J Basic Clin Physiol Pharmacol | Anti-atherosclerotic activity of aqueous extract of Ipomoea batatas (L.) leaves in high-fat diet-induced atherosclerosis model rats [Pubmed:34986543] |
| Stem Cell Res Ther | A dual role of HIF1α in regulating osteogenesis–angiogenesis coupling [Pubmed:35123567] |
| American Journal of Translational Research | Curcumin treats endometriosis in mice by the HIF signaling pathway [Pubmed:35559378] |
| Clinical and Experimental Immunology | Clinical significance of differential serum-signatures for early prediction of severe dengue among Eastern Indian patients [Pubmed:35348620] |
| Medicina | Assessment of the RANTES Level Correlation and Selected Inflammatory and Pro-Angiogenic Molecules Evaluation of Their Influence on CRC Clinical Features: A … [Pubmed:35208526] |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … [Pubmed:35585377] |
| Biomaterials Advances | Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats [] |
| Biointerface Research in Applied Chemistry | Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats [] |
| Research Square | DMOG attenuates steroid-associated endothelial progenitor cell impairment and osteonecrosis of the femoral head via regulating HIF-1α signaling pathway in … [] |
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