Ⅱ型前胶原羧基端原肽(PIICP)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Procollagen II C-Terminal Propeptide (PIICP)
P2CP; C-Propeptide Of Type II Procollagen; Procollagen II Carboxy Terminal Propeptide
- 编号SEA964Eq
- 物种Equus caballus; Equine (Horse，马) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围62.5-4,000pg/mL
- 灵敏度最小可检测剂量小于等于25.4pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3251 ¥ 4644 ¥ 20898 ¥ 39474 ¥ 325080
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测Ⅱ型前胶原羧基端原肽(PIICP)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的Ⅱ型前胶原羧基端原肽(PIICP)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 90-97 | 94 |
| EDTA plasma(n=5) | 90-97 | 93 |
| heparin plasma(n=5) | 94-105 | 102 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的Ⅱ型前胶原羧基端原肽(PIICP)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中Ⅱ型前胶原羧基端原肽(PIICP)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-105% | 80-99% | 88-103% | 81-99% |
| EDTA plasma(n=5) | 89-99% | 83-103% | 98-105% | 96-105% |
| heparin plasma(n=5) | 93-101% | 91-99% | 94-101% | 98-105% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将Ⅱ型前胶原羧基端原肽(PIICP)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的Ⅱ型前胶原羧基端原肽(PIICP)与连接于固相载体上的抗体结合，然后加入生物素化的Ⅱ型前胶原羧基端原肽(PIICP)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Ⅱ型前胶原羧基端原肽(PIICP)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Equus caballus; Equine (Horse，马) | 应用(仅供研究使用，不用于临床诊断！) |
| SEA964Eq | Ⅱ型前胶原羧基端原肽(PIICP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA964Eq | Ⅱ型前胶原羧基端原肽(PIICP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Experimental and Therapeutic Medicine | Evaluation of the effect of N-acetyl-glucosamine administration on biomarkers for cartilage metabolism in healthy individuals without symptoms of arthritis: A … [10.3892] |
| Functional Foods in Health & Disease | Evaluation of the effect of administering N-acetyl-glucosamine-containing green tea supplement on biomarkers for cartilage metabolism in healthy individuals without symptoms of arthritis: a randomized double-blind placebo-controlled clinical study [309] |
| EXPERIMENTAL AND THERAPEUTIC MEDICINE | Effect of N-acetylglucosamine administration on cartilage metabolism and safety in healthy subjects without symptoms of arthritis: A case report [pubmed:28413518] |
| Experimental and Therapeutic Medicine | Evaluation of the effect of salmon nasal proteoglycan on biomarkers for cartilage metabolism in individuals with knee joint discomfort: A randomized double‑blind placebo‑controlled clinical study [etm:14] |
| Experimental and Therapeutic Medicine | Evaluation of the efficacy of Ajuga decumbens extract supplement in individuals with knee discomfort associated with physical activity: A randomized, double‑blind, placebo‑controlled study [10.3892/etm.2017.5064] |
| EXPERIMENTAL AND THERAPEUTIC MEDICINE | Evaluation of the chondroprotective action of N‑acetylglucosamine in a rat experimental osteoarthritis [pubmed:28912864] |
| Functional Foods In Health And Disease | Evaluation of the effect of N-acetyl-glucosamine administration on biomarkers for cartilage metabolism in healthy individuals: a randomized double-blind placebo-controlled clinical study [view/366] |
| Spine | No effects of hyperosmolar culture medium on tissue regeneration by human degenerated nucleus pulposus cells despite upregulation extracellular matrix genes [fulltext:2018/03010] |
| Advances in Clinical and Experimental Medicine | Fibulin-3 and other cartilage metabolism biomarkers in relationship to calprotectin (MRP8/14) and disease activity in rheumatoid arthritis patients treated with … [68362.pdf] |
| Functional Food Research | サケ鼻軟骨由来プロテオグリカン摂取による関節保護効果 [] |
| Spine | No Effects of Hyperosmolar Culture Medium on Tissue Regeneration by Human Degenerated [Pubmed: 25856264] |
| Applied Physiology Nutrition and Metabolism | Effectiveness of collagen supplementation on pain scores in healthy individuals with self-reported knee pain; A randomized controlled trial [Pubmed: 31990581] |
| International Journal of Molecular Sciences | Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration [Pubmed: 32295299] |
| Biomolecules | Concerted Actions by PIICP, CTXII, and TNF-¦Á in Patients with Juvenile Idiopathic Arthritis [33924892] |
