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神经元特异性烯醇化酶(NSE)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Enolase, Neuron Specific (NSE)

ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase

  • 神经元特异性烯醇化酶(NSE)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 神经元特异性烯醇化酶(NSE)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 神经元特异性烯醇化酶(NSE)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA537Rb.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测神经元特异性烯醇化酶(NSE),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的神经元特异性烯醇化酶(NSE)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 93-101 96
EDTA plasma(n=5) 80-90 83
heparin plasma(n=5) 80-101 97

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的神经元特异性烯醇化酶(NSE),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中神经元特异性烯醇化酶(NSE)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 90-97% 96-105% 84-105% 86-96%
EDTA plasma(n=5) 95-105% 91-105% 91-98% 87-96%
heparin plasma(n=5) 90-98% 82-93% 90-104% 79-101%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将神经元特异性烯醇化酶(NSE)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的神经元特异性烯醇化酶(NSE)与连接于固相载体上的抗体结合,然后加入生物素化的神经元特异性烯醇化酶(NSE)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的神经元特异性烯醇化酶(NSE)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Oryctolagus cuniculus (Rabbit,兔) 应用(仅供研究使用,不用于临床诊断!)
RPA537Rb01 神经元特异性烯醇化酶(NSE)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA537Rb51 神经元特异性烯醇化酶(NSE)多克隆抗体 WB; IHC; ICC; IP.
MAA537Rb21 神经元特异性烯醇化酶(NSE)单克隆抗体 WB; IHC; ICC; IP.
SEA537Rb 神经元特异性烯醇化酶(NSE)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA537Rb 神经元特异性烯醇化酶(NSE)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Journal of Neurotrauma The Anti-Inflammatory and Neuroprotective Effects of Ghrelin in Subarachnoid Hemorrhage-Induced Oxidative Brain Damage in Rats [Liebert: 20091210]
20 Melatonin treatment protects against spinal cord injury induced functional and biochemical changes in rat urinary bladder [Wiley: source]
Journal of Pharmacology and Toxicology Ginkgo biloba Enhances the Anticonvulsant and Neuroprotective Effects of Sodium Valproate Against Kainic Acid-induced Seizures in Mice [Medwell: 2011679690]
Addiction Biology Comparative gene expression profiling analysis of lymphoblastoid cells reveals neuron‐specific enolase gene (ENO2) as a susceptibility gene of heroin dependence [PubMed: 21995595]
Placenta Advanced glycation end products as an upstream molecule triggers ROS-induced sFlt-1 production in extravillous trophoblasts: A novel bridge between oxidative stress and preeclampsia [Pubmed: 24144948]
J Ethnopharmacol. Therapeutic time window and underlying therapeutic mechanism of breviscapine injection against cerebral ischemia/reperfusion injury in rats. [Pubmed: 24291152]
J Neurosurg. Detection of β-amyloid oligomers as a predictor of neurological outcome after brain injury. [Pubmed: 23540266]
Analytical Methods A portable and quantitative enzyme immunoassay of neuron-specific enolase with a glucometer read out [Rsc: Source]
Int J Neurosci. The effect of therapeutic hypothermia after cardiopulmonary resuscitation on ICAM-1 and NSE levels in sudden cardiac arrest rabbits [Pubmed:25111247]
Experimental & Molecular Medicine Elevated levels of serum sICAM-1 in asphyxiated low birth weight newborns [Pubmed:25358349]
Neuroscience Letters Preconditioning effect of (S)-3,5-dihydroxyphenylglycine on ischemic injury in middle cerebral artery occluded Sprague-Dawley rats. [Pubmed:25562631]
中华临床医师杂志 高迁移率族蛋白B1和神经元烯醇化酶对心脏骤停大鼠复苏后脑损伤评价的研究 [Journal: Fulltext]
Am J Trop Med Hyg Blood–Brain Barrier Function and Biomarkers of Central Nervous System Injury in Rickettsial versus Other Neurological Infections in Laos [PubMed: 26055741]
Neurosci Lett Preconditioning effect of (S)-3, 5-dihydroxyphenylglycine on ischemic injury in middle cerebral artery occluded Sprague–Dawley rats [PubMed: 25562631]
Physiol Rep S100B and NSE serum concentrations after simulated diving in rats [PubMed: 26462746]
Int J Mol Sci The effect of electroporation of a lyotroic liquid crystal Genistein-based formulation in the recovery of murine melanoma lesions [PubMed: 26184156]
PLoS One Isoflurane Post-Treatment Improves Outcome after an Embolic Stroke in Rabbits [PubMed: 26645542]
Фармакология, клиническая фармакология Волгоградский государственный медицинский университет [uploads:dsovet]
Analytical Methods Synthesis of sunflower-like gold nanostructures and their application in electrochemical immunoassays using the nanogold-triggered hydrogen evolution reaction [articlelanding:c5ay03174e]
中国临床药理学与治疗学 右美托咪定对脑膜瘤切除术患者血清S100β 蛋白及神经元特异性烯醇化酶的影响 [article:9794]
Asian Pacific Journal of Tropical Medicine Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injury [science:S1995764516300955]
Izvorni znanstveni članak SERUMSKA RAZINA ZA NEURON SPECIFIČNE ENOLAZE KAO BILJEG ISHEMIJSKO-REPERFUZIJSKOG OŠTEĆENJA U BOLESNIKA PODVRGNUTIH KAROTIDNOJ ENDARTEREKTOMIJI [262238]
Neurourol Urodyn Functional and structural changes of the urinary bladder following spinal cord injury; treatment with alpha lipoic acid [pubmed:27490041]
Biomedicine & Pharmacotherapy Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in rats [pubmed:28076830]
cellular physiology and biochemistry Hydrogen-Rich Saline Attenuates Brain Injury Induced by Cardiopulmonary Bypass and Inhibits Microvascular Endothelial Cell Apoptosis Via the PI3K/Akt/GSK3β … [pubmed:29040978]
Brain Research Intra-arterial human urinary kallidinogenase alleviates brain injury in rats with permanent middle cerebral artery occlusion through PI3K/AKT/FoxO1 signaling pathway [Pubmed:29510144]
Biomed Research International Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway [Pubmed:29770336]
Journal of the Neurological Sciences S100B, NSE and MMP-9 fail to predict neurologic outcome while elevated S100B associates with milder initial clinical presentation after aneurysmal subarachnoid … [Pubmed:29801873]
Iranian Journal of Veterinary Research Nitrosative stress indices in dogs with neurological form of canine distemper [Doi: 10.22099/IJVR.2018.4943]
Evidence-Based Complementary and Alternative Medicine Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway []
The Eurasian Journal of Medicine Effects of Conivaptan versus Mannitol on Post-Ischemic Brain Injury and Edema []
Medicina The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the … []
ACTA NEUROBIOLOGIAE EXPERIMENTALIS Neuroprotective effect of poly (lactic-co-glycolic acid) nanoparticle-bound brain-derived neurotrophic factor in a permanent middle cerebral artery occlusion … [Pubmed: 32214270]
Stem Cells International Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 … [Pubmed: 32300366]
European Review for Medical and Pharmacological Sciences Neuroprotective effect of CTRP3 overexpression against sevoflurane anesthesia-induced cognitive dysfunction in aged rats through activating AMPK/SIRT1 and … [Pubmed: 32432773]
Neurobiology of Learning and Memory Effects of Circadian Rhythm Disorder on the Hippocampus of SHR and WKY Rats []
The role of light desynchronosis in the development of stress-induced aging []
ИЗУЧЕНИЕ ЦЕРЕБРОПРОТЕКТОРНОГО ДЕЙСТВИЯ ПРЕПАРАТОВ РАЗЛИЧНЫХ ФАРМАКОЛОГИЧЕСКИХ ГРУПП В УСЛОВИЯХ … []
Chinese Journal of Analytical Chemistry Investigation of Library Input on Aptamers Selection Efficiency Using Capillary Electrophoresis []
Ther Adv Chronic Dis The effect of doxycycline on neuron-specific enolase in patients with traumatic brain injury: a randomized controlled trial [34262678]
J Surg Res Perillaldehyde Alleviates Spinal Cord Ischemia-Reperfusion Injury Via Activating the Nrf2 Pathway [34399353]
Cephalalgia Evaluation of serum neuron specific enolase levels among patients with primary and secondary burning mouth syndrome [34644195]
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