α2-HS糖蛋白(AHSG)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Alpha-2-Heremans Schmid Glycoprotein (AHSG)
aHSG; HSGa; A2HS; AHS; FETUA; HSG-A2; Fetuin-A; Alpha 2-HS Glycoprotein; Fetuin A; Alpha-2-Z-Globulin; Ba-alpha-2-Glycoprotein
- 编号SEA178Ra
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围6.25-400ng/mL
- 灵敏度最小可检测剂量小于等于2.55ng/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2873 ¥ 4104 ¥ 18468 ¥ 34884 ¥ 287280
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测α2-HS糖蛋白(AHSG)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的α2-HS糖蛋白(AHSG)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 79-93 | 85 |
| EDTA plasma(n=5) | 91-98 | 95 |
| heparin plasma(n=5) | 78-101 | 98 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的α2-HS糖蛋白(AHSG)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中α2-HS糖蛋白(AHSG)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-88% | 78-94% | 91-101% | 78-101% |
| EDTA plasma(n=5) | 94-101% | 79-91% | 92-101% | 85-96% |
| heparin plasma(n=5) | 83-97% | 93-101% | 80-101% | 81-102% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将α2-HS糖蛋白(AHSG)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的α2-HS糖蛋白(AHSG)与连接于固相载体上的抗体结合，然后加入生物素化的α2-HS糖蛋白(AHSG)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的α2-HS糖蛋白(AHSG)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA178Ra01 | α2-HS糖蛋白(AHSG)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA178Ra01 | α2-HS糖蛋白(AHSG)多克隆抗体 | WB; IHC; ICC; IP. |
| FAA178Ra01 | 抗α2-HS糖蛋白(AHSG)多克隆抗体 | Flow cytometry. |
| MAA178Ra21 | α2-HS糖蛋白(AHSG)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA178Ra | α2-HS糖蛋白(AHSG)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA178Ra | α2-HS糖蛋白(AHSG)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA178Ra01 | α2-HS糖蛋白(AHSG)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Journal of Proteomics | Proteomic analysis reveals oxidative stress response as the main adaptative physiological mechanism in cows under different production systems [ScienceDirect: S1874391912002084] |
| Journal of Physiology and Biochemistry | Curcumin prevents liver fat accumulation and serum fetuin-A increase in rats fed a high-fat diet [PubMed: 23430567] |
| BioMed Research International | Serum Fetuin-A Levels Related with Microalbuminuria in Diet-Induced Obese Rats [PubMed: PMC3591142] |
| Osteoporosis International | Estrogen increases the transcription of human α2-Heremans-Schmid-glycoprotein by an interplay of estrogen receptor α and activator protein-1. [Pubmed: 24504099] |
| BMC Pharmacology and Toxicology | A novel 11β-hydroxysteroid dehydrogenase type1 inhibitor CNX-010-49 improves hyperglycemia, lipid profile and reduces body weight in diet induced obese C57B6/J mice with a potential to provide cardio protective benefits [Pubmed:25098735] |
| Acta Dermatovenerologica Croatica | Relationship Between Serum Fetuin-A Levels and Carotid Intima-media Thickness in Turkish Patients with Mild to Moderate Psoriasis. A Case-control Study [PubMed: 26476900] |
| Mol Cell Endocrinol | Fetuin A promotes lipotoxicity in β cells through the TLR4 signaling pathway and the role of pioglitazone in anti-lipotoxicity. [PubMed: 25986658] |
| Ann Dermatol | Evaluation of Arterial Stiffness in Patients with Beh [PubMed: 26719639] |
| Endocrine | Visceral and subcutaneous adipose tissue express and secrete functional alpha2hsglycoprotein (fetuin a) especially in obesity [pubmed:27738888] |
| Diabetes Care | Targeted Microbiome Intervention by Microencapsulated Delayed-Release Niacin Beneficially Affects Insulin Sensitivity in Humans [pubmed:29212824] |
| JOURNAL OF ALZHEIMERS DISEASE | Acute Anti-Inflammatory Markers ITIH4 and AHSG in Mice Brain of a Novel Alzheimer's Disease Model [Pubmed: 30958362] |
| Journal of Alzheimer's Disease | A New Serum Biomarker Set to Detect Mild Cognitive Impairment and Alzheimer's Disease by Peptidome Technology [Pubmed: 31771070] |
| Sci Rep | Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309] |
| J Obstet Gynaecol Res | Predıctıve value of pregnancy of follıcular fluıd fetuın‐A and‐B levels ın infertıle women after intra‐cytoplasmic sperm injection [34708901] |
