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酪氨酸羟化酶(TH)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Tyrosine Hydroxylase (TH)

TYH; DYT5b; Tyrosine 3-Monooxygenase; Dystonia 14

  • 酪氨酸羟化酶(TH)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 酪氨酸羟化酶(TH)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 酪氨酸羟化酶(TH)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEB438Ra.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测酪氨酸羟化酶(TH)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将酪氨酸羟化酶(TH)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的酪氨酸羟化酶(TH)与连接于固相载体上的抗体结合，然后加入生物素化的酪氨酸羟化酶(TH)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的酪氨酸羟化酶(TH)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
RPB438Ra01 酪氨酸羟化酶(TH)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB438Ra01 酪氨酸羟化酶(TH)多克隆抗体 WB; IHC; ICC; IP.
LAB438Ra71 酪氨酸羟化酶(TH)多克隆抗体(生物素标记) WB; IHC; ICC.
MAB438Ra25 酪氨酸羟化酶(TH)单克隆抗体 WB; IHC; ICC; IP.
MAB438Ra21 酪氨酸羟化酶(TH)单克隆抗体 WB; IHC; ICC; IP.
MAB438Ra22 酪氨酸羟化酶(TH)单克隆抗体 WB; IHC; ICC; IP.
MAB438Ra27 酪氨酸羟化酶(TH)单克隆抗体 WB; IHC; ICC; IP.
MAB438Ra23 酪氨酸羟化酶(TH)单克隆抗体 WB; IHC; ICC; IP.
MAB438Ra24 酪氨酸羟化酶(TH)单克隆抗体 WB; IHC; ICC; IP.
SEB438Ra 酪氨酸羟化酶(TH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB438Ra 酪氨酸羟化酶(TH)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMB438Ra 酪氨酸羟化酶(TH)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB438Ra01 酪氨酸羟化酶(TH)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

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PLOS ONE Genetic Diagnosis of Two Dopa-Responsive Dystonia Families by Exome Sequencing [Pubmed:25181484]
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Therapeutics and Clinical Risk Management Elevated blood plasma levels of epinephrine, norepinephrine, tyrosine hydroxylase, TGFβ1, and TNFα associated with high-altitude pulmonary edema in an Indian population. [pubmed:27540296]
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Journal of Cellular Biochemistry Vaccarin administration ameliorates hypertension and cardiovascular remodeling in renovascular hypertensive rats [pubmed:28681939]
Environmental Science & Technology Multigenerational disruption of the thyroid endocrine system in marine medaka after a life-cycle exposure to perfluorobutanesulfonate [Pubmed:29565584]
Molecular Neurobiology Cilostazol Mediated Nurr1 and Autophagy Enhancement: Neuroprotective Activity in Rat Rotenone PD Model [Pubmed:29429051]
Chemosphere Tetrabromobisphenol A caused neurodevelopmental toxicity via disrupting thyroid hormones in zebrafish larvae [Pubmed:29407805]
Neurospine The Effect of Recombinant Tyrosine Hydroxylase Expression on the Neurogenic Differentiation Potency of Mesenchymal Stem Cells [Pubmed:29656620]
Toxicology and Applied Pharmacology Montelukast attenuates rotenone-induced microglial activation/p38 MAPK expression in rats: Possible role of its antioxidant, anti-inflammatory and antiapoptotic effects [Pubmed: 30222980]
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Environmental Pollution Embryonic exposure to pentabromobenzene inhibited the inflation of posterior swim bladder in zebrafish larvae [Pubmed: 31935612]
International Journal of Pharmacology Nose to brain delivery of rotigotine loaded chitosan nanoparticles in human SH-SY5Y neuroblastoma cells and animal model of Parkinson's disease [Pubmed: 32084576]
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Ecotoxicol Environ Saf Effects of SiO2 nanoparticles on the uptake of tetrabromobisphenol A and its impact on the thyroid endocrine system in zebrafish larvae [33385677]
Nutr Res Tocotrienols protect differentiated SH-SY5Y human neuroblastoma cells against 6-hydroxydopamine-induced cytotoxicity by ameliorating dopamine biosynthesis and … [Pubmed:35065349]
Food Chem Toxicol Omarigliptin attenuates rotenone-induced Parkinson's disease in rats: Possible role of oxidative stress, endoplasmic reticulum stress and immune modulation [Pubmed:35439590]
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Journal of Environmental Sciences Parental and transgenerational impairments of thyroid endocrine system in zebrafish by 2, 4, 6-tribromophenol []
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