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S100钙结合蛋白A7(S100A7)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for S100 Calcium Binding Protein A7 (S100A7)

S100-A7; PSOR1; S100A7c; Psoriasin 1

  • S100钙结合蛋白A7(S100A7)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • S100钙结合蛋白A7(S100A7)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • S100钙结合蛋白A7(S100A7)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEC035Bo.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测S100钙结合蛋白A7(S100A7),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的S100钙结合蛋白A7(S100A7)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 90-98 95
EDTA plasma(n=5) 84-98 89
heparin plasma(n=5) 80-91 83

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的S100钙结合蛋白A7(S100A7),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中S100钙结合蛋白A7(S100A7)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 80-99% 82-101% 89-96% 85-92%
EDTA plasma(n=5) 90-97% 93-105% 88-95% 91-101%
heparin plasma(n=5) 88-96% 92-101% 88-103% 91-99%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将S100钙结合蛋白A7(S100A7)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的S100钙结合蛋白A7(S100A7)与连接于固相载体上的抗体结合,然后加入生物素化的S100钙结合蛋白A7(S100A7)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的S100钙结合蛋白A7(S100A7)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Bos taurus; Bovine (Cattle,牛) 应用(仅供研究使用,不用于临床诊断!)
RPC035Bo02 S100钙结合蛋白A7(S100A7)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC035Bo61 S100钙结合蛋白A7(S100A7)活性蛋白 Cell culture; Activity Assays.
RPC035Bo01 S100钙结合蛋白A7(S100A7)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPC035Bo61 S100钙结合蛋白A7(S100A7)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC035Bo02 S100钙结合蛋白A7(S100A7)多克隆抗体 WB
PAC035Bo01 S100钙结合蛋白A7(S100A7)多克隆抗体 WB; IHC; ICC; IP.
SEC035Bo S100钙结合蛋白A7(S100A7)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
KSC035Bo01 S100钙结合蛋白A7(S100A7)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It(ELISA Kit) Yourself"

参考文献

杂志 参考文献
Archives of Dermatological Research Serum levels of antimicrobial peptides and proteins do not correlate with psoriasis severity and are increased after treatment with fumaric acid esters [SpringerLink: t07u24t43r357l15]
J Proteome Res Proteomic Signature of Endothelial Dysfunction Identified in the Serum of Acute Ischemic Stroke Patients by the iTRAQ-Based LC–MS Approach [PubMed: 25807139]
Exp Dermatol S100A7 (psoriasin) inhibits human epidermal differentiation by enhanced IL-6 secretion through IκB/NF-κB signaling [Pubmed:27060579]
Experimental Dermatology S100A7 (psoriasin) inhibits human epidermal differentiation by enhanced IL‐6 secretion through IκB/NF‐κB signalling [pubmed:27060579]
Experiental And Therapeutic Medicine Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription [pubmed:28672924]
Scientific Reports The psoriasis-associated IL-17A induces and cooperates with IL-36 cytokines to control keratinocyte differentiation and function. [pubmed:29142248]
Journal of the European Academy of Dermatology and Venereology A potential contribution of psoriasin to vascular and epithelial abnormalities and inflammation in systemic sclerosis [Pubmed:28681537]
Scientific Reports Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique [Pubmed:29374283]
Neuroscience Bulletin Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative state [Pubmed:30019218]
Journal of Proteomics Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection [Pubmed: 31706945]
Mediators of Inflammation Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris []
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