硒蛋白P1(SEPP1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Selenoprotein P1, Plasma (SEPP1)
SEPP; SeP; SEP-P1; SELP
- 编号SEB809Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.78-50ng/mL
- 灵敏度最小可检测剂量小于等于0.31ng/mL.
- 样本类型serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2793 ¥ 3990 ¥ 17955 ¥ 33915 ¥ 279300
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测硒蛋白P1(SEPP1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的硒蛋白P1(SEPP1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 86-102 | 94 |
| EDTA plasma(n=5) | 82-101 | 87 |
| heparin plasma(n=5) | 90-105 | 95 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的硒蛋白P1(SEPP1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中硒蛋白P1(SEPP1)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-95% | 89-103% | 97-105% | 92-99% |
| EDTA plasma(n=5) | 80-90% | 92-102% | 82-104% | 81-96% |
| heparin plasma(n=5) | 79-93% | 94-102% | 84-93% | 86-102% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将硒蛋白P1(SEPP1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的硒蛋白P1(SEPP1)与连接于固相载体上的抗体结合，然后加入生物素化的硒蛋白P1(SEPP1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的硒蛋白P1(SEPP1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB809Hu01 | 硒蛋白P1(SEPP1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB809Hu01 | 硒蛋白P1(SEPP1)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB809Hu81 | 硒蛋白P1(SEPP1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAB809Hu71 | 硒蛋白P1(SEPP1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAB809Hu25 | 硒蛋白P1(SEPP1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB809Hu22 | 硒蛋白P1(SEPP1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB809Hu23 | 硒蛋白P1(SEPP1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB809Hu24 | 硒蛋白P1(SEPP1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB809Hu21 | 硒蛋白P1(SEPP1)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB809Hu | 硒蛋白P1(SEPP1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB809Hu | 硒蛋白P1(SEPP1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| The Journal of Clinical Endocrinology & Metabolism | Serum Selenoprotein P Levels in Patients with Type 2 Diabetes and Prediabetes: Implications for Insulin Resistance, Inflammation, and Atherosclerosis [PubMed: 21677040] |
| Diabetes & metabolism journal | Increased Selenoprotein P Levels in Subjects with Visceral Obesity and Nonalcoholic Fatty Liver Disease [PubMed: 23439771] |
| Journal of Pediatric Biochemistry | Trace elements, heavy metals and vitamins in Egyptian school children with iron deficiency anemia [Metapress:Source] |
| Journal of Trace Elements in Medicine and Biology | Selenium and its relationship with selenoprotein P and glutathione peroxidase in children and adolescents with Hashimoto's thyroiditis and hypothyroidism [PubMed: 26854239] |
| OBES RES CLIN PRACT/OBESITY RESEARCH & CLINICAL PRACTICE | Selenoprotein P is elevated in individuals with obesity, but is not independently associated withinsulin resistance. [pubmed:27524654] |
| Annals of Nutrition and Metabolism | Acute Overfeeding Does Not Alter Liver or Adipose Tissue-Derived Cytokines in HealthyHumans. [pubmed:27832637] |
| European Journal of Nutrition | DNA damage and oxidative stress response to selenium yeast in the non-smoking individuals: ashort-term supplementation trial with respect to GPX1 and SEPP1 polymorphism. [pubmed:26658762] |
| South. Clin. Ist. Euras. | Evaluation of the Relationship Between Insulin Resistance and Selenoprotein P in Patients with Polycystic Ovary Syndrome [DOI: 10.14744/scie.2017.87597] |
| Arch Endocrinol Metab | Serum selenium and selenoprotein-P levels in autoimmune thyroid dieases patients in a select center a transversal study [DOI: 10.1590/2359-3997000000309] |
| British Journal of Nutrition | Selenium, selenoproteins and selenometabolites in mothers and babies at the time of birth [pubmed:28534447] |
| Journal of Trace Elements in Medicine and Biology | Biomarkers of selenium status and antioxidant effect in workers occupationally exposed to mercury [Pubmed:29895371] |
| Biological & Pharmceutical Bulletin | Comparison of Human Selenoprotein P Determinants in Serum between Our Original Methods and Commercially Available Kits [Pubmed:29709922] |
| Diabetologia | Non-alcoholic fatty liver disease in the first trimester and subsequent development of gestational diabetes mellitus [Pubmed: 30470912] |
| Biological Trace Element Research | Selenium Levels in Community Dwellers with Type 2 Diabetes Mellitus [Pubmed: 30725267] |
| Advances in Medical Sciences | Can hepatokines be regarded as novel non-invasive serum biomarkers of intrahepatic lipid content in obese children? [Pubmed: 30921653] |
| Acute swimming exercise, but not exposure to moderate hypoxic conditions reduces circulating selenoprotein P levels in short-term, high-fat diet-fed rats [Pubmed: 31236416] | |
| Hormones | Selenium and selenoprotein P in nonalcoholic fatty liver disease [Pubmed: 31493247] |
| Bull Environ Contam Toxicol | Soil Selenium Concentration and Residents Daily Dietary Intake in a Selenosis Area: A Preliminary Study in Yutangba Village, Enshi City, China [Pubmed: 32909074] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Higher Serum Selenoprotein P Level as a Novel Inductor of Metabolic Complications in Psoriasis [Pubmed: 32605214] |
| Circulating hepassocin level in patients with stable angina is associated with fatty liver and renal function [] | |
| Nat Immunol | Selenium–GPX4 axis protects follicular helper T cells from ferroptosis [34413521] |
| Reviews in Cardiovascular Medicine | Selenoprotein P-1 (SEPP1) as An Early Biomarker of Acute Kidney Injury in Patients Undergoing Cardiopulmonary Bypass [] |
