沉默调节蛋白1(SIRT1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Sirtuin 1 (SIRT1)
SIR2L1; Silent Mating Type Information Regulation 2 Homolog 1; NAD-Dependent Deacetylase Sirtuin-1; SIR2-like protein 1; SirtT1 75 kDa fragment
- 编号SEE912Mu
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.312-20ng/mL
- 灵敏度最小可检测剂量小于等于0.122ng/mL.
- 样本类型tissue homogenates, cell lysates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3024 ¥ 4320 ¥ 19440 ¥ 36720 ¥ 302400
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测沉默调节蛋白1(SIRT1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将沉默调节蛋白1(SIRT1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的沉默调节蛋白1(SIRT1)与连接于固相载体上的抗体结合，然后加入生物素化的沉默调节蛋白1(SIRT1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的沉默调节蛋白1(SIRT1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPE912Mu01 | 沉默调节蛋白1(SIRT1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPE912Mu02 | 沉默调节蛋白1(SIRT1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAE912Mu01 | 沉默调节蛋白1(SIRT1)多克隆抗体 | WB; IHC; ICC; IP. |
| PAE912Mu02 | 沉默调节蛋白1(SIRT1)多克隆抗体 | WB; IHC; ICC; IP. |
| LAE912Mu71 | 沉默调节蛋白1(SIRT1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEE912Mu | 沉默调节蛋白1(SIRT1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LME912Mu | 沉默调节蛋白1(SIRT1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSE912Mu01 | 沉默调节蛋白1(SIRT1)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
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| PLoS One. | SIRT1 Gene Polymorphisms Affect the Protein Expression in Cardiovascular Diseases [Plos: Source] |
| PLOS ONE | A Novel Role for SIRT-1 in L-Arginine Protection against STZ Induced Myocardial Fibrosis in Rats [Pubmed:25501750] |
| Journal of Alzheimer's Disease | Serum miR-206 and miR-132 as Potential Circulating Biomarkers for Mild Cognitive Impairment [Pubmed:25589731] |
| Respirology | Increases in peripheral SIRT1: A new biological characteristic of asthma [PubMed: 26040995] |
| PLOS ONE | A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly: [PubMed: 25785999] |
| Endocrine | Changes of serum Mir-217 and the correlation with the severity in type 2 diabetes patients with different stages of diabetic kidney disease. [pubmed:27522360] |
| Naunyn Schmiedebergs Arch Pharmacol | The potential curative effect of rebamipide in hepatic ischemia/reperfusion injury [pubmed:28361247] |
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| International Journal of Cardiology | Serum concentrations and gene expression of sirtuin 1 in healthy and slightly overweight subjects after caloric restriction or resveratrol supplementation: A randomized trial [pubmed:28029409] |
| Hepatology International | Association of SIRT1 gene polymorphism and its expression for the risk of alcoholic fatty liver disease in the Han population [pubmed:29189974] |
| Toxicology and Applied Pharmacology | Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1) [pubmed:28974454] |
| Chemico-biological Interactions | Magnolol protects pancreatic β-cells against methylglyoxal-induced cellular dysfunction [pubmed:28919305] |
| Nutrients | Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after … [Pubmed:30037068] |
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| Kidney and Blood Pressure Research | The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy [Pubmed: 30504713] |
| Journal of International Medical Research | Serum and follicular fluid levels of sirtuin 1, sirtuin 6, and resveratrol in women undergoing fertilization: an observational, clinical study [Pubmed: 30556451] |
| BioMed Research International | Centella asiatica Prevents Increase of Hippocampal Tumor Necrosis Factor-Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of Chronic … [] |
| 学位论文 | Influência do tratamento da doença periodontal na sirtuína 1 e na lectina ligadora de manose em indivíduos com doença arterial coronária [] |
| Journal of Physics D: Applied Physics | Premature Myocardial Infarction: Genetic Variations in SIRT1 Affect Disease Susceptibility [] |
| Journal of Geriatric Oncology | Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors [Pubmed: 30952517] |
| Biomed Research International | Prevents Increase of Hippocampal Tumor Necrosis Factor-α Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of … [Pubmed: 30956976] |
| Journal of Diabetes and its Complications | Vasohibin-1 in T2DM with different UACR [] |
| Clinical Interventions in Aging | The Serum Concentration of Anti-Aging Proteins, Sirtuin1 and αKlotho in Patients with End-Stage Kidney Disease on Maintenance Hemodialysis [Pubmed: 32214805] |
| Compr Psychiatry | Crocin alleviates schizophrenia-like symptoms in rats by upregulating silent information regulator-1 and brain derived neurotrophic factor [Pubmed: 33045669] |
| Сибирский федеральный университет | Влияние гречневой крупы на сиртуиновую активность у подопытных животных в эксперименте [] |
| Journal of Cereal Science | Dietary buckwheat enhances sirtuin1 without calorie restriction [] |
| Clin Interv Aging | Molecular Changes in Circulating microRNAs' Expression and Oxidative Stress in Adults with Mild Cognitive Impairment: A Biochemical and Molecular Study [33447019] |
| Antioxidants | The Effect of Repeated Whole-Body Cryotherapy on Sirt1 and Sirt3 Concentrations and Oxidative Status in Older and Young Men Performing Different Levels of?¡ [33396247] |
| Int Urol Nephrol | Evaluation of sirtuin 1 (SIRT1) levels in autosomal dominant polycystic kidney disease [33864594] |
| Neurochem Int | Dimethyl fumarate abridged tauo-/amyloidopathy in a D-Galactose/ovariectomy-induced Alzheimer's-like disease: Modulation of AMPK/SIRT-1, AKT/CREB/BDNF, AKT ¡ [34052296] |
| Toxicol Appl Pharmacol | Prasugrel anti-ischemic effect in rats: Modulation of hippocampal SUMO2/3-IкBα/Ubc9 and SIRT-1/miR-22 trajectories [34174262] |
| Food Chem | Buckwheat-enriched diet alleviates bisphenol A mediated oxidative stress via modulation of sirtuin 1 and antioxidant status in experimental rats [34763933] |
| Life Sci | Novel mechanistic insights towards the repositioning of alogliptin in Parkinson's disease [34774622] |
| Int J Mol Sci | Intralesional Infiltrations of Arteriosclerotic Tissue Cells-Free Filtrate Reproduce Vascular Pathology in Healthy Recipient Rats [Pubmed:35163435] |
| J Clin Med | Effects of Coffee on Sirtuin-1, Homocysteine, and Cholesterol of Healthy Adults: Does the Coffee Powder Matter? [Pubmed:35683374] |
| Cell & Bioscience | OGT Protects Skeletal Muscle From Damage Through SIRT1 O-GlcNAcylation During Cold Stress [] |
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