27kDa热休克蛋白(Hsp27)重组蛋白
Recombinant Heat Shock Protein 27 (Hsp27)
HSPB1; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock 27kDa Protein 1; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;
- 编号RPA693Mu01
- 物种Mus musculus (Mouse,小鼠) 相同的名称,不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU/µg(LAL法测定)
- 亚细胞定位细胞核, 细胞质
- 预测分子量24.5kDa
- 实际分子量28kDa(差异分析请参阅说明书)
- 片段与标签Met1~Lys209 with N-terminal His Tag
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性状冻干粉
- 纯度> 95%
- 等电点6.4
-
应用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,请参见活性蛋白。 - 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 912 ¥ 2280 ¥ 4560 ¥ 13680 ¥ 34200
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
编号 | 适用物种:Mus musculus (Mouse,小鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPA693Mu02 | 27kDa热休克蛋白(Hsp27)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA693Mu01 | 27kDa热休克蛋白(Hsp27)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA693Mu01 | 27kDa热休克蛋白(Hsp27)多克隆抗体 | WB; IHC; ICC; IP. |
PAA693Mu02 | 27kDa热休克蛋白(Hsp27)多克隆抗体 | WB; IHC; ICC; IP. |
SEA693Mu | 27kDa热休克蛋白(Hsp27)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA693Mu | 27kDa热休克蛋白(Hsp27)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA693Mu01 | 27kDa热休克蛋白(Hsp27)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
杂志 | 参考文献 |
Animal Science Journal | The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cells [PubMed: 26467738] |
Nature Communications | HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis [Pubmed:29650953] |
Advances in Experimental Medicine and Biology | Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease [Pubmed:29429028] |
Diabetes Research and Clinical Practice | The beneficial effects of 15 units of high-intensity circuit training in women is modified by age, baseline insulin resistance and physical capacity [Pubmed: 31102684] |
Journal of Immunology Research | Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients [] |
Journal of Clinical Medicine | Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort [Pubmed: 32210109] |
Medicine | Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive … [Pubmed:35687771] |
Beilstein Archives | Immunization of mice with gold nanoparticles conjugated to thermostable tumor antigens prevents tumor development during transplantation [] |
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