白蛋白(ALB)检测试剂盒(酶联免疫吸附试验法,高敏型)
High Sensitive ELISA Kit for Albumin (ALB)
Serum albumin
- 编号HEB028Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围1.56-100ng/mL
- 灵敏度最小可检测剂量小于等于0.55ng/mL.
- 样本类型serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 1945 ¥ 2778 ¥ 12501 ¥ 23613 ¥ 194460
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测白蛋白(ALB)检测试剂盒(酶联免疫吸附试验法,高敏型)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的白蛋白(ALB)检测试剂盒(酶联免疫吸附试验法,高敏型)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 90-98 | 95 |
| EDTA plasma(n=5) | 79-91 | 80 |
| heparin plasma(n=5) | 80-91 | 88 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的白蛋白(ALB)检测试剂盒(酶联免疫吸附试验法,高敏型)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中白蛋白(ALB)检测试剂盒(酶联免疫吸附试验法,高敏型)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-102% | 80-104% | 96-105% | 80-96% |
| EDTA plasma(n=5) | 86-96% | 79-103% | 91-99% | 79-88% |
| heparin plasma(n=5) | 90-101% | 85-98% | 83-90% | 79-101% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将白蛋白(ALB)检测试剂盒(酶联免疫吸附试验法,高敏型)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的白蛋白(ALB)检测试剂盒(酶联免疫吸附试验法,高敏型)与连接于固相载体上的抗体结合，然后加入生物素化的白蛋白(ALB)检测试剂盒(酶联免疫吸附试验法,高敏型)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的白蛋白(ALB)检测试剂盒(酶联免疫吸附试验法,高敏型)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| NPB028Hu01 | 白蛋白(ALB)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| NPB028Hu02 | 白蛋白(ALB)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB028Hu01 | 白蛋白(ALB)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB028Hu51 | 白蛋白(ALB)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB028Hu51 | 白蛋白(ALB)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB028Hu07 | 白蛋白(ALB)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB028Hu05 | 白蛋白(ALB)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB028Hu51 | 白蛋白(ALB)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB028Hu01 | 白蛋白(ALB)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB028Hu02 | 白蛋白(ALB)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB028Hu71 | 白蛋白(ALB)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAB028Hu81 | 白蛋白(ALB)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAB028Hu22 | 白蛋白(ALB)单克隆抗体 | WB; IHC |
| MAB028Hu21 | 白蛋白(ALB)单克隆抗体 | WB; IHC; ICC; IP. |
| HEB028Hu | 白蛋白(ALB)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEB028Hu | 白蛋白(ALB)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEB028Hu | 抗白蛋白抗体(Anti-ALB)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| ACB028Hu | 抗白蛋白抗体(Anti-ALB)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antibody Detection. |
| CCB028Hu | 白蛋白(ALB)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB028Hu | 白蛋白(ALB)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB028Hu11 | 白蛋白(ALB)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| PLoS One | Simvastatin Reduces Endotoxin-Induced Acute Lung Injury by Decreasing Neutrophil Recruitment and Radical Formation [PubMed: 22701728] |
| Liver International | Albumin administration protects against bilirubin-induced auditory brainstem dysfunction in Gunn rat pups [Onlinelibrary: liv.12219] |
| Journal of Infectious Diseases | Adjunctive corticosteroid therapy improves lung immunopathology and survival during severe secondary pneumococcal pneumonia in mice [Pubmed: 24273183] |
| Toxins (Basel). | Increased Circulating Levels of Vitamin D Binding Protein in MS Patients [Pubmed:25590278] |
| Mol Cell Biochem | The wnt/β-catenin signaling pathway participates in rhein ameliorating kidney injury in DN mice [PubMed: 26346164] |
| PLoS One | Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human Study [Pubmed:26934615] |
| Journal of Biomaterials and Tissue Engineering | 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering [art00007] |
| Experimental and molecular pathology | Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. [pubmed:29246788] |
| Inflammation | Resolvin D1 Promotes SIRT1 Expression to Counteract the Activation of STAT3 and NF-κB in Mice with Septic-Associated Lung Injury [Pubmed:30014231] |
| Journal of Developmental Origins of Health and Disease | Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspring [Pubmed:29582727] |
| Medicine | Electret filter collects more exhaled albumin than glass condenser: A method comparison based on human study [Pubmed:29384875] |
| Stem Cell Research & Therapy | Therapeutic efficiency of human amniotic epithelial stem cell-derived functional hepatocyte-like cells in mice with acute hepatic failure [Pubmed: 30463600] |
| JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | Decellularized Human Liver Scaffold‐Based Three‐dimensional Culture System Facilitate HBV Infection [Pubmed: 30963688] |
| biomedicine & pharmacotherapy | Treatment with 3, 4-dihydroxyphenylethyl alcohol glycoside ameliorates sepsis-induced ALI in mice by reducing inflammation and regulating M1 polarization [Pubmed: 31146107] |
| biochemical and biophysical research communications | Anti-proteinuria effect of antibody against ANGPTL3 coil-coiled domain on adriamycin-induced nephropathy in mice [Pubmed: 31256934] |
| Diabetes, Metabolic Syndrome and Obesity | A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activation [Pubmed: 31447572] |
| Molecular and Cellular Endocrinology | Diiodothyronines regulate metabolic homeostasis in primary human hepatocytes by modulating mTORC1 and mTORC2 activity [Pubmed: 31580898] |
| BMC Veterinary Research | Preliminary study on the effects of enrofloxacin, flunixin meglumine and pegbovigrastim on Mycoplasma bovis pneumonia [Pubmed: 31655595] |
| International Immunopharmacology | Ghrelin alleviates traumatic brain injury-induced acute lung injury through pyroptosis/NF-κB pathway [Pubmed: 31918060] |
| HYPERTENSION RESEARCH | Sodium butyrate ameliorates deoxycorticosterone acetate/salt-induced hypertension and renal damage by inhibiting the MR/SGK1 pathway [Pubmed: 32908237] |
| Obesity Surgery | Sleeve Gastrectomy Attenuates Diabetic Nephropathy by Upregulating Nephrin Expressions in Diabetic Obese Rats [Pubmed: 32399849] |
| Protective Role of Training and Selenium Consumption Against Renal Toxicity Induced by Cadmium in Rats [] | |
| Nutrition | Gut Dysbiosis Contribute to High Fructose Induced Salt Sensitive Hypertension In Sprague-Dawley Rats [] |
| sensors and actuators b-chemical | A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate [] |
| Biochem Biophys Res Commun | cGAS promotes sepsis in radiotherapy of cancer by up-regulating caspase-11 signaling [33721834] |
| Journal of Chinese Pharmaceutical Sciences | A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle [] |
| Inflammation | Syringaresinol Resisted Sepsis-Induced Acute Lung Injury by Suppressing Pyroptosis Via the Oestrogen Receptor-β Signalling Pathway [34807349] |
| Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313] |
| ACS Appl Mater Interfaces | Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions [34914377] |
| Food & Function | Moderation of gut microbiota and bile acid metabolism by chlorogenic acid improves high-fructose-induced salt-sensitive hypertension in mice [Pubmed:35708145] |
| Authorea | Syringaresinol attenuates kidney injury by inhibition of pyroptosis via activating the Nrf2 antioxidant pathway in diabetic nephropathy [] |
