表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法,高敏型)
High Sensitive ELISA Kit for Epidermal Growth Factor (EGF)
URG; Beta-Urogastrone; Pro-epidermal growth factor
- 编号HEA560Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围1.56-100pg/mL
- 灵敏度最小可检测剂量小于等于0.53pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2302 ¥ 3288 ¥ 14796 ¥ 27948 ¥ 230160
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法,高敏型)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法,高敏型)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 92-99 | 96 |
| EDTA plasma(n=5) | 94-102 | 98 |
| heparin plasma(n=5) | 84-92 | 89 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法,高敏型)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法,高敏型)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-97% | 89-96% | 80-93% | 79-94% |
| EDTA plasma(n=5) | 82-95% | 82-99% | 86-101% | 85-96% |
| heparin plasma(n=5) | 79-94% | 81-88% | 90-101% | 82-105% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法,高敏型)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法,高敏型)与连接于固相载体上的抗体结合，然后加入生物素化的表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法,高敏型)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法,高敏型)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA560Hu02 | 表皮生长因子(EGF)活性蛋白 | Cell culture; Activity Assays. |
| RPA560Hu03 | 表皮生长因子(EGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA560Hu08 | 表皮生长因子(EGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA560Hu09 | 表皮生长因子(EGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA560Hu02 | 表皮生长因子(EGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA560Hu06 | 表皮生长因子(EGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA560Hu01 | 表皮生长因子(EGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA560Hu01 | 表皮生长因子(EGF)活性蛋白 | Cell culture; Activity Assays. |
| PAA560Hu05 | 表皮生长因子(EGF)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA560Hu01 | 表皮生长因子(EGF)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA560Hu02 | 表皮生长因子(EGF)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA560Hu71 | 表皮生长因子(EGF)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA560Hu22 | 表皮生长因子(EGF)单克隆抗体 | WB; IHC |
| MAA560Hu24 | 表皮生长因子(EGF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA560Hu23 | 表皮生长因子(EGF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA560Hu25 | 表皮生长因子(EGF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA560Hu27 | 表皮生长因子(EGF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA560Hu21 | 表皮生长因子(EGF)单克隆抗体 | WB; IHC; ICC; IP. |
| HEA560Hu | 表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA560Hu | 表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| WEA560Hu | 表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法,宽范围) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA560Hu | 表皮生长因子(EGF)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA560Hu | 表皮生长因子(EGF)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PSA560Hu01 | 表皮生长因子(EGF)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA560Hu01 | 表皮生长因子(EGF)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
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| Biomark Insights | Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism [PubMed: PMC3623607] |
| Plos one | The Probiotic Mixture VSL#3 Accelerates Gastric Ulcer Healing by Stimulating Vascular Endothelial Growth Factor [PubMed: PMC3590171] |
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| Journal of Controlled Release | A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injury [pubmed:28522195] |
| Scientific Reports | Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets [pubmed:28811665] |
| International Journal of Nanomedicine | Micro-/nano-topography of selective laser melting titanium enhances adhesion and proliferation and regulates adhesion-related gene expressions of human … [Pubmed: 30233172] |
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| Stem Cells International | The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin?¡ [33381190] |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … [Pubmed:35585377] |
| Biomaterials Advances | Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats [] |
| Authorea | Syringaresinol attenuates kidney injury by inhibition of pyroptosis via activating the Nrf2 antioxidant pathway in diabetic nephropathy [] |
| Biointerface Research in Applied Chemistry | Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats [] |
