Ⅲ型胶原(COL3)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Collagen Type III (COL3) ,etc. by FLIA (Flow Luminescence Immunoassay)
（注：单次混测多因子不超过8个指标 ）
- 编号LMA176Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围0.039-40ng/mL
- 灵敏度最小可检测剂量小于等于0.013ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 8指标数 7指标数 6指标数 5指标数 4指标数 3指标数 2指标数1指标数
- 价格 ¥ 2980 ¥ 3094 ¥ 3266 ¥ 3495 ¥ 3725 ¥ 4068 ¥ 4584 ¥ 5730 添加到价格计算器 计算器
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测Ⅲ型胶原(COL3)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的Ⅲ型胶原(COL3)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 78-96 | 92 |
| EDTA plasma(n=5) | 79-92 | 87 |
| heparin plasma(n=5) | 78-97 | 85 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的Ⅲ型胶原(COL3)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中Ⅲ型胶原(COL3)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-99% | 80-103% | 95-103% | 97-104% |
| EDTA plasma(n=5) | 92-101% | 80-94% | 96-105% | 95-103% |
| heparin plasma(n=5) | 78-102% | 83-99% | 97-105% | 96-103% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将Ⅲ型胶原(COL3)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的Ⅲ型胶原(COL3)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的Ⅲ型胶原(COL3)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的Ⅲ型胶原(COL3)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| PAA176Hu01 | Ⅲ型胶原(COL3)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA176Hu71 | Ⅲ型胶原(COL3)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA176Hu | Ⅲ型胶原(COL3)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA176Hu | Ⅲ型胶原(COL3)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| WEA176Hu | Ⅲ型胶原(COL3)检测试剂盒(酶联免疫吸附试验法,宽范围) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA176Hu | 抗Ⅲ型胶原抗体(Anti-COL3)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| LMA176Hu | Ⅲ型胶原(COL3)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| nephrology dialysis transplantation | Triptolide inhibits extracellular matrix protein synthesis by suppressing the Smad2 but not the MAPK pathway in TGF-β1-stimulated NRK-49F cells [OxfordJournals: 3180] |
| Acta Otolaryngol | Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa [PubMed: 19958244] |
| Bulletin of Experimental Biology and Medicine | Modification of collagen formation by mesenchymal stem cells isolated from human adipose tissue in culture and after autotransplantation for abdominal hernia plasty [Pubmed: 24319714] |
| Age( dordr). | In vitro tenocyte metabolism in aging and oestrogen deficiency [Springer: Source] |
| Stem Cell Research Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly [Pubmed: 24739658] |
| International Journal of Advanced Research | Effect of nandrolone and/or whey protein on the soleus muscle and testis of adult male albino rats [Com:Source] |
| PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media [Pubmed:Pmc4130592] |
| Connective Tissue Research | Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes [Pubmed:25333747] |
| Laryngoscope. | Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria [Pubmed:25545625] |
| Cardiology | KLF15 is an essential negative regulatory factor for the cardiac remodeling response to pressure overload [Pubmed:25633973] |
| physiological research | The skin remodeling in type 1 diabetes and insulin resistance animal models [PubMed: 26047379] |
| Physiol Res | The skin remodeling in type 1 diabetes and insulin resistance animal models. [PubMed: 26047379] |
| Archives of Plastic Surgery | Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas [PubMed: 26430624] |
| Life Sci | Pituitary tumor transforming gene as a novel regulatory factor of liver fibrosis [PubMed: 25936962] |
| Molecular and Cellular Biochemistry | SVVYGLR motif of the thrombin-cleaved N-terminal osteopontin fragment enhances the synthesis of collagen type III in myocardial fibrosis [PubMed: 26112906] |
| Cardiology | KLF16 Is an Essential Negative Regulatory Factor for the Cardiac Remodeling Response to Pressure Overload [PubMed: 25633973] |
| Int J Clin Exp Med | Uighur medicine abnormal savda munzip (ASMq) suppresses expression of collagen and TGF-β1 with concomitant induce Smad7 in human hypertrophic scar … [PubMed: 26309506] |
| scientific reports | IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy [Pubmed:27263531] |
| Кардіологія | Інфаркт міокарда, ускладнений аневризмою лівого шлуночка: клінічні і структурно-функціональні особливості перебігу, діагностичні, терапевтичні та прогностичні аспекти [bitstream:123456789] |
| International Journal of Molecular Sciences | Plasma LncRNA-ATB, a Potential Biomarker for Diagnosis of Patients with Coal Workers'Pneumoconiosis: A Case-Control Study. [pubmed:27556453] |
| BMC Cancer | Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent andmitochondrial pathways in mammalian hepatic stellate cells. [pubmed:27461108] |
| Sci Rep. | Genomic reprograming analysis of the Mesothelial to Mesenchymal Transition identifies biomarkers in peritoneal dialysis patients. [pubmed:28327551] |
| The American Journal of Chinese Medicine | Saikosaponin a Induces Apoptosis through Mitochondria-Dependent Pathway in Hepatic Stellate Cells [pubmed:28231747] |
| Biomedical and Environmental Sciences | Crystalline Silica Promotes Rat Fibrocyte Differentiation in Vitro, and Fibrocytes Participate in Silicosis in Vivo [pubmed:29081339] |
| Oxidative Medicine and Cellular Longevity | Effects of the (Pro) renin Receptor on Cardiac Remodeling and Function in a Rat Alcoholic Cardiomyopathy Model via the PRR-ERK1/2-NOX4 Pathway [] |
| Cytokine | Effect of proinflammatory cytokines on endometrial collagen and metallopeptidase expression during the course of equine endometrosis [Pubmed: 31265984] |
| ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | Pantoea agglomerans chronic exposure induces epithelial-mesenchymal transition in human lung epithelial cells and mice lungs [Pubmed: 32146192] |
| in vitro cellular & developmental biology-animal | Distinct differences in hypoxic responses between human oral mucosa and skin fibroblasts in a 3D collagen matrix [Pubmed: 32588253] |
| ACS Biomaterials Science & Engineering | Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction [] |
| Stem Cell Research & Therapy | Enhanced cell survival and therapeutic benefits of IL-10-expressing multipotent mesenchymal stromal cells for muscular dystrophy [33541428] |
| journal of biomedical materials research part B | Cell sheet technology: Influence of culture conditions on in vitro©\cultivated corneal stromal tissue for regenerative therapies of the ocular surface [33538123] |
| PLoS One | Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies [33999928] |
| BMC MOLECULAR BIOLOGY | Uncarboxylated osteocalcin alleviates the inhibitory effect of high glucose on osteogenic differentiation of mouse bone marrow¨Cderived mesenchymal stem?¡ [33906607] |
| Russian Journal of Cardiology | Early diagnosis of myocardial fibrosis in patients with epicardial obesity [] |
| Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction [] |