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无翅型MMTV整合位点家族成员5A(WNT5A)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) ,etc. by FLIA (Flow Luminescence Immunoassay)

(注:单次混测多因子不超过8个指标 )

  • 无翅型MMTV整合位点家族成员5A(WNT5A)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 无翅型MMTV整合位点家族成员5A(WNT5A)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒对检测无翅型MMTV整合位点家族成员5A(WNT5A)等多因子检测试剂盒(流式荧光发光法)具有较高的灵敏度。
经检测人、小鼠、大鼠之间有100%的交叉反映。

回收率

分别于定值血清及血浆样本中加入一定量的无翅型MMTV整合位点家族成员5A(WNT5A)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 89-96 92
EDTA plasma(n=5) 95-105 101
heparin plasma(n=5) 96-105 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的无翅型MMTV整合位点家族成员5A(WNT5A)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中无翅型MMTV整合位点家族成员5A(WNT5A)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 83-90% 95-105% 88-101% 88-96%
EDTA plasma(n=5) 78-93% 80-97% 82-89% 98-105%
heparin plasma(n=5) 90-101% 96-104% 95-104% 92-101%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠)标准品或样本100μL及磁珠10μL,
    37°C酶标板振荡器孵育90分钟;
3. 磁吸甩干,加检测溶液A100μL,37°C酶标板振荡器孵育60分钟;
4. 磁吸洗板3次;
5. 加检测溶液B100μL,37°C振动孵育30分钟;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋涡震荡2分钟后读数。

实验原理

将无翅型MMTV整合位点家族成员5A(WNT5A)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠,其中的无翅型MMTV整合位点家族成员5A(WNT5A)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合,然后加入生物素化的无翅型MMTV整合位点家族成员5A(WNT5A)等多因子检测试剂盒(流式荧光发光法)抗体,将未结合的生物素化抗体洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的无翅型MMTV整合位点家族成员5A(WNT5A)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种:Homo sapiens (Human,人), Mus musculus (Mouse,小鼠), Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
SEP549Mi 无翅型MMTV整合位点家族成员5A(WNT5A)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCP549Mi 无翅型MMTV整合位点家族成员5A(WNT5A)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMP549Mi 无翅型MMTV整合位点家族成员5A(WNT5A)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
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Liver Secreted frizzled‐related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis [PubMed: 25488180]
Transl Res Wnt5a is expressed in spondyloarthritis and exerts opposite effects on enthesis and bone in murine organ and cell cultures [PubMed: 26163991]
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