1,25-二羟基维生素D3(DHVD3)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for 1,25-Dihydroxyvitamin D3 (DHVD3) ,etc. by FLIA (Flow Luminescence Immunoassay)
DH-VD3; Calcitriol; Rocaltrol; Calcijex; 1,25-(OH)2D3; 1,25-Dihydroxycholecalciferol; 1,25-Dihydroxy Vitamin D3
（注：单次混测多因子不超过8个指标 ）
- 编号LMA467Ge
- 物种Pan-species (General，通用) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长1.5h
- 检测范围1.95-2000pg/mL
- 灵敏度最小可检测剂量小于等于0.65 pg/mL.
- 样本类型Serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测1,25-二羟基维生素D3(DHVD3)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的1,25-二羟基维生素D3(DHVD3)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 95-104 | 98 |
| EDTA plasma(n=5) | 78-95 | 83 |
| heparin plasma(n=5) | 87-95 | 91 |
| sodium citrate plasma(n=5) | 95-103 | 101 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的1,25-二羟基维生素D3(DHVD3)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中1,25-二羟基维生素D3(DHVD3)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-101% | 91-104% | 94-102% | 85-98% |
| EDTA plasma(n=5) | 94-102% | 87-94% | 94-102% | 87-95% |
| heparin plasma(n=5) | 80-94% | 88-95% | 99-105% | 84-104% |
| sodium citrate plasma(n=5) | 87-101% | 80-92% | 82-92% | 88-103% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将1,25-二羟基维生素D3(DHVD3)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的1,25-二羟基维生素D3(DHVD3)等多因子检测试剂盒(流式荧光发光法)和未标记的1,25-二羟基维生素D3(DHVD3)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的1,25-二羟基维生素D3(DHVD3)等多因子检测试剂盒(流式荧光发光法)呈负相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| CEA467Ge | 1,25-二羟基维生素D3(DHVD3)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA467Ge | 1,25-二羟基维生素D3(DHVD3)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| PLoS One | Selective Cytotoxic Action and DNA Damage by Calcitriol-Cu(II) Interaction: Putative Mechanism of Cancer Prevention [PubMed: PMC3785422] |
| BMC Medicine | Calcium-deficiency assessment and biomarker identification by an integrated urinary metabonomics analysis [PubMed: PMC3652781] |
| Nephrol Dial Transplant. | Treatment of established left ventricular hypertrophy with fibroblast growth factor receptor blockade in an animal model of CKD [Pubmed:24875663] |
| Endocrinology. | Vitamin D Prevents Podocyte Injury via Regulation of Macrophage M1/M2 Phenotype in Diabetic Nephropathy Rats [Pubmed:25188527] |
| Tumor Biology | Calcitriol?induced?redox?imbalance and DNA breakage in cells sharing a common metabolic feature of malignancies: Interaction with cellular copper (II) ions leads to the production of reactive oxygen species. [Pubmed:25547436] |
| International Journal of Life Sciences Research | Impact of Curcumin and Flaxseed Oil on Bone Turnover in Glucocorticoid-Injected Female Rats [profile:303311214] |
| Protective Effect of Soybean and Mushroom against Glucocorticoid-Induced Osteoporosis in Female Rats [:] | |
| Apoptosis | Calcitriol–copper interaction leads to non enzymatic, reactive oxygen species mediated DNA breakage and modulation of cellular redox scavengers in hepatocellular … [article:10.1007] |
| in vivo | Effect of Calcitriol on FGF23 Level in Healthy Adults and its Dependence on Phosphate Level [pubmed:28064234] |
| Hypertension Research | Glucocorticoid exposure induces preeclampsia via dampening 1,25-dihydroxyvitamin D3 [pubmed:29093562] |
| International Journal of Clinical and Experimental Medicine | Combined effect of pulsed electromagnetic fields and narrowband ultraviolet B on bone metabolism in glucocorticoid-treated rats [] |
| Journal of Clinical and Translational Endocrinology | Dobesity: relationship between vitamin d deficiency, obesity and sclerostin as a novel biomarker of bone metabolism [] |
| International Journal of Environmental Research and Public Health | Alleviating Effect of α-Lipoic Acid and Magnesium on Cadmium-Induced Inflammatory Processes, Oxidative Stress and Bone Metabolism Disorders in Wistar Rats [Pubmed: 31739465] |
| Ecotoxicology and Environmental Safety | Sodium dehydroacetate induces cardiovascular toxicity associated with Ca2+ imbalance in zebrafish [] |
| EXPERIMENTAL AND MOLECULAR MEDICINE | Metabolic activities affect femur and lumbar vertebrae remodeling, and anti-resorptive risedronate disturbs femoral cortical bone remodeling [33436949] |
| Life Sci | SWIM domain protein ZSWIM4 is required for JAK2 inhibition resistance in breast cancer [34102191] |
| Nutrients | Effect of Dapagliflozin and Magnesium Supplementation on Renal Magnesium Handling and Magnesium Homeostasis in Metabolic Syndrome [34836340] |