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血管紧张素原(AGT)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Angiotensinogen (AGT) ,etc. by FLIA (Flow Luminescence Immunoassay)

ANHU; SERPINA8; Angiotensin; Renin Substrate; Serpin Peptidase Inhibitor,Clade A,Member 8(Alpha-1 Antiproteinase,Antitrypsin)

（注：单次混测多因子不超过8个指标 ）

  • 血管紧张素原(AGT)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 血管紧张素原(AGT)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测血管紧张素原(AGT)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的血管紧张素原(AGT)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 84-92 88
EDTA plasma(n=5) 83-91 87
heparin plasma(n=5) 84-93 89

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的血管紧张素原(AGT)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血管紧张素原(AGT)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 85-93% 78-94% 98-105% 87-102%
EDTA plasma(n=5) 97-105% 85-99% 91-101% 83-94%
heparin plasma(n=5) 92-105% 87-104% 78-92% 82-94%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
    37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将血管紧张素原(AGT)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的血管紧张素原(AGT)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的血管紧张素原(AGT)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的血管紧张素原(AGT)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPA797Hu02 血管紧张素原(AGT)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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APA797Hu61 血管紧张素原(AGT)活性蛋白 Cell culture; Activity Assays.
EPA797Hu61 血管紧张素原(AGT)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA797Hu01 血管紧张素原(AGT)多克隆抗体 WB; IHC; ICC; IP.
PAA797Hu02 血管紧张素原(AGT)多克隆抗体 WB; IHC; ICC; IP.
MAA797Hu22 血管紧张素原(AGT)单克隆抗体 WB; IHC; ICC; IP.
SEA797Hu 血管紧张素原(AGT)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA797Hu 血管紧张素原(AGT)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA797Hu 血管紧张素原(AGT)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA797Hu 血管紧张素原(AGT)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA797Hu01 血管紧张素原(AGT)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Transplant International Elevated urinary angiotensinogen a marker of intrarenal renin angiotensin system in hypertensive renal transplant recipients: does it play a role in development of proteinuria in hypertensive renal transplant patients? [Wiley: source]
Amyloid The relation between urinary angiotensinogen and proteinuria in renal AA amyloidosis patients [PubMed: 22320202]
Pediatric Mephrology Urinary angiotensinogen as a marker of intrarenal angiotensin II activity in adolescents with primary hypertension [PubMed: PMC3661928]
Acta Paediatrica Urinary angiotensinogen as a novel marker of obstructive nephropathy in children [Pubmed: 23772991]
Journal of the Renin-Angiotensin-Aldosterone System Association between urinary angiotensinogen, hypertension and proteinuria in pregnant women with preeclampsia [Sagepub: Source]
Pediatric Nephrology MP707 CHARACTERISTICS AND OUTCOMES OF CHILDREN WITH AUTOSOMAL RECESSIVE POLYCYSTIC KIDNEY DISEASE COHORT FROM THE ESPN/ERA-EDTA REGISTRY [Oxfordjournals:Source]
journal of human hypertension Relationship between blood pressure var [PubMed: 26223347]
PLoS One Higher Serum Angiotensinogen Is an Indicator of IgA Vasculitis with Nephritis Revealed by Comparative Proteomes Analysis [PubMed: 26098644]
MKA Korelasi pengukuran antropometri dengan tekanan darah dan angiotensinogen plasma pada dewasa [Index.Php: Art]
Journal of Human Hypertension Relationship between blood pressure variability and renal activity of the renin-angiotensin system. [pubmed:26223347]
Acta Pharmacol Sin. Pharmacological evidence: a new therapeutic approach to the treatment of chronic heart failure through SUR2B/Kir6.1 channel in endothelial cells. [pubmed:27890915]
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
Life Sciences Pyrrolidine dithiocarbamate inhibits mouse acute kidney injury induced by diclofenac by targeting oxidative damage, cytokines and NF-κB activity [Pubmed:30036488]
American Journal of Physiology-Renal Physiology Role of (Pro) Renin Receptor in Albumin Overload-Induced Nephropathy in Rats [Pubmed:29846109]
Methods in Molecular Biology Quantitation of Total and Free Thiol Angiotensinogen as a Prognostic Marker for Preeclampsia [Pubmed: 31069778]
J Transl Med Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans [33407555]
Hypertens Res Association of Ang-(1¨C7) and des-Arg 9 BK as new biomarkers of obesity and cardiometabolic risk factors in adolescents [33568792]
Physiol Rep (Pro) renin receptor antagonist PRO20 attenuates nephrectomy©\induced nephropathy in rats via inhibition of intrarenal RAS and Wnt/¦Â©\catenin signaling [34057312]
Cancer Cell Int A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer [34215253]
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