太阳集团tyc5997_平台(china)官方网站

脑钠素(BNP)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Brain Natriuretic Peptide (BNP) ,etc. by FLIA (Flow Luminescence Immunoassay)

GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32

（注：单次混测多因子不超过8个指标 ）

  • 脑钠素(BNP)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 脑钠素(BNP)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测脑钠素(BNP)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的脑钠素(BNP)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 96-105 101
EDTA plasma(n=5) 78-94 85
heparin plasma(n=5) 81-91 87

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的脑钠素(BNP)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中脑钠素(BNP)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 78-97% 83-91% 85-101% 81-103%
EDTA plasma(n=5) 88-95% 94-102% 92-101% 79-101%
heparin plasma(n=5) 94-101% 88-101% 99-105% 93-101%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
    37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将脑钠素(BNP)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的脑钠素(BNP)等多因子检测试剂盒(流式荧光发光法)和未标记的脑钠素(BNP)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的脑钠素(BNP)等多因子检测试剂盒(流式荧光发光法)呈负相关。

赠品

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
RPA541Ra01 脑钠素(BNP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA541Ra01 脑钠素(BNP)多克隆抗体 WB; IHC; ICC; IP.
MAA541Ra21 脑钠素(BNP)单克隆抗体 WB; IHC; ICC; IP.
MAA541Ra22 脑钠素(BNP)单克隆抗体 WB; IHC; ICC; IP.
SEA541Ra 脑钠素(BNP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
WEA541Ra 脑钠素(BNP)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA541Ra 脑钠素(BNP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA541Ra 脑钠素(BNP)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA541Ra11 脑钠素(BNP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Heart and Circulatory Physiology B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sickness [APS: source]
PLoS One Renalase deficiency in heart failure model of rats_a potential mechanism underlying circulating norepinephrine accumulation. [PubMed: 21297953]
International Journal of Toxicology Cardiac Troponin T and NT-proBNP as Biomarkers of Early Myocardial Damage in Amitriptyline-Induced Cardiovascular Toxicity in Rats [Pubmed: 24055824]
JACC ELEVATED ST2 SERUM LEVELS A BIOMARKER FOR CARDIOMYOPATHY IN DUCHENNE MUSCULAR DYSTROPHY [Onlinejacc:Source]
J Urol.? Why does Adenotonsillectomy Not Correct Enuresis in All Children with Sleep Disordered Breathing? [Pubmed:24679871]
Life Sci Cardioprotective effects of baicalein on heart failure via modulation of Ca2 [PubMed: 26706290]
Vlaams Diergeneeskundig Tijdschrift Equine BNP measurement using a porcine BNP enzyme-linked immunoassay: a pilot study [Publication: 5950761]
Physiological Research Renal Sympathetic Denervation Improves Cardiac Dysfunction [PubMed: 25804096]
Life Sci Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitro [Pubmed:26706290]
J Ethnopharmacol Danhong injection attenuates isoproterenol-induced cardiac hypertrophy by reCavia (Guinea pig )lating p38 and NF-κb pathway [Pubmed:26970569]
Echocardiography. Improvement in the prediction of exercise-induced elevation of left ventricular filling pressure in patients with normal left ventricular ejection fraction. [pubmed:27779336]
SpringerPlus The effect of long-term amiodarone administration on myocardial fibrosis and evolution of left ventricular remodeling in a porcine model of ischemic … [pubmed:27652141]
Heart Lung Circ. Efficacy and Safety of Renal Sympathetic Denervation on Dogs with Pressure Overload-Induced Heart Failure. [pubmed:27555054]
Clinica Chimica Acta  Plasma concentration of fibronectin is decreased in patients with hypertrophic cardiomyopathy [pubmed:27693530]
Scientific Reports Paracrine cross-talk between skeletal muscle and macrophages in exercise by PGC-1α-controlled BNP. [pubmed:28091624]
Molecular Medicine Report Intermedin improves cardiac function and sympathetic neural remodeling in a rat model of post myocardial infarction heart failure [pubmed:28627670]
PLoS One Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy [pubmed:29240788]
Acta Physiologica Beneficial Effects of Renal Denervation on Cardiac Angiogenesis in Rats with Prolonged Pressure Overload [pubmed:27575955]
International Immunopharmacology Metformin attenuated endotoxin-induced acute myocarditis via activating AMPK [pubmed:28410530]
Drug Delivery Ginsenoside Rg3 micelles mitigate doxorubicin-induced cardiotoxicity and enhance its anticancer efficacy. [pubmed:29063791]
Science of the Total Environment Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity and [Pubmed:29727991]
Scientific Reports An essential role for Wnt/β-catenin signaling in mediating hypertensive heart disease [Pubmed:29895976]
International Journal of Cardiology Cardioprotective effect of substance P in a porcine model of acute myocardial infarction [Pubmed:29884290]
International Journal of Cardiology High-intensity interval training enhances mitochondrial bioenergetics of platelets in patients with heart failure [Pubmed:30072155]
Evidence-based Complementary and Alternative Medicine  Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects [Pubmed:29977313]
Molecular Medicine Reports  Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats [Pubmed:29956781]
Pediatric Cardiology Determinants of Increased Aortic Diameters in Young Normotensive Patients With Turner Syndrome Without Structural Heart Disease [Pubmed:29392348]
BMC Pharmacology and Toxicology Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis [Pubmed:29321061]
中国病理生理杂志 和肽素水平对大鼠心肾综合征的早期预测价值 [filename:ZBLS201802013]
FASEB Journal Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure [Pubmed: 30230922]
Toxicology Mechanisms and Methods Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats [Pubmed: 30426860]
Van Veterinary Journal Van Veterinary Journal []
Kidney International Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndrome [Pubmed: 30770217]
Journal of Cellular Physiology Inflammatory cytokines associated with cancer growth induce mitochondria and cytoskeleton alterations in cardiomyocytes [Pubmed: 30982981]
Cardiology Circulating Connective Tissue Growth Factor Is Associated with Diastolic Dysfunction in Patients with Diastolic Heart Failure [Pubmed: 31466059]
BIOMEDICINE & PHARMACOTHERAPY Pharmacodynamic and pharmacokinetic effects of S086, a novel angiotensin receptor neprilysin inhibitor [Pubmed: 32570118]
biochimica et biophysica acta-molecular basis of disease Empagliflozin improves left ventricular diastolic function of db/db mice [Pubmed: 32353614]
Dapagliflozin: a Sodium-glucose Cotransporter 2 Inhibitor, Attenuates Angiotensin II-induced Cardiac Fibrotic Remodeling by Regulating TGF¦Â1/Smad ¡­ [34116674]
Radiation-induced heart energy metabolism dysfunction leads to heart fibrosis []
Oxid Med Cell Longev κ Opioid Receptor Agonist Inhibits Myocardial Injury in Heart Failure Rats through Activating Nrf2/HO-1 Pathway and Regulating Ca2+-SERCA2a [34373768]
Mol Med Rep Radiation‑induced dysfunction of energy metabolism in the heart results in the fibrosis of cardiac tissues [34633055]
viruses Coxsackievirus A2 Leads to Heart Injury in a Neonatal Mouse Model [34452454]
Peptides Osteocrin alleviates cardiac hypertrophy via attenuating oxidative stress [Pubmed:35218795]
留言咨询
Baidu
sogou