胆囊收缩素(CCK)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Cholecystokinin (CCK) ,etc. by FLIA (Flow Luminescence Immunoassay)
CCK-PZ
(注:单次混测多因子不超过8个指标 )
- 编号LMA802Ra
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 实验方法竞争抑制
- 反应时长1.5h
- 检测范围0.78-800pg/mL
- 灵敏度最小可检测剂量小于等于0.26 pg/mL.
- 样本类型Serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 8指标数 7指标数 6指标数 5指标数 4指标数 3指标数 2指标数1指标数
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特异性
本试剂盒用于检测胆囊收缩素(CCK)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的胆囊收缩素(CCK)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 81-96 | 93 |
EDTA plasma(n=5) | 98-105 | 102 |
heparin plasma(n=5) | 97-105 | 101 |
sodium citrate plasma(n=5) | 92-101 | 98 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
线性
在定值血清及血浆样本内加入适量的胆囊收缩素(CCK)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中胆囊收缩素(CCK)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 83-97% | 82-103% | 93-101% | 99-105% |
EDTA plasma(n=5) | 83-101% | 91-102% | 79-101% | 80-96% |
heparin plasma(n=5) | 88-102% | 83-93% | 82-99% | 83-93% |
sodium citrate plasma(n=5) | 96-105% | 97-104% | 97-104% | 95-105% |
稳定性
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠、检测溶液A)标准品或样本50μL及磁珠10μL,加检测溶液A50μL,
37°C酶标板振荡器孵育60分钟;
3. 磁吸洗板3次;
4. 加检测溶液B100μL,37°C振动孵育30分钟;
5. 磁吸洗板3次;
6. 加鞘液100μL,旋涡震荡2分钟后读数。
实验原理
将胆囊收缩素(CCK)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠、标记VEGFA,标记的胆囊收缩素(CCK)等多因子检测试剂盒(流式荧光发光法)和未标记的胆囊收缩素(CCK)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合,然后将未结合物洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的胆囊收缩素(CCK)等多因子检测试剂盒(流式荧光发光法)呈负相关。
赠品
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPA802Ra01 | 胆囊收缩素(CCK)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA802Ra01 | 胆囊收缩素(CCK)多克隆抗体 | WB; IHC; ICC; IP. |
LAA802Ra71 | 胆囊收缩素(CCK)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAA802Ra29 | 胆囊收缩素(CCK)单克隆抗体 | WB; IHC; ICC; IP. |
MAA802Ra27 | 胆囊收缩素(CCK)单克隆抗体 | WB; IHC; ICC; IP. |
MAA802Ra25 | 胆囊收缩素(CCK)单克隆抗体 | WB; IHC; ICC; IP. |
MAA802Ra21 | 胆囊收缩素(CCK)单克隆抗体 | WB; IHC |
MAA802Ra23 | 胆囊收缩素(CCK)单克隆抗体 | WB; IHC; ICC; IP. |
MAA802Ra22 | 胆囊收缩素(CCK)单克隆抗体 | WB; IHC; ICC; IP. |
MAA802Ra24 | 胆囊收缩素(CCK)单克隆抗体 | WB; IHC; ICC; IP. |
MEA802Ra | 胆囊收缩素(CCK)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEA802Ra | 胆囊收缩素(CCK)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA802Ra | 胆囊收缩素(CCK)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
杂志 | 参考文献 |
Journal of Obstetrics and Gynaecology Research? | Disturbed release of cholecystokinin in pregnant women with hyperemesis gravidarum [Pubmed:25331205] |
World J Gastroenterol | Effects of sphincter of Oddi motility on the formation of cholesterol gallstones [Pubmed:27350732] |
Journal of Ethnopharmacology | Luhong formula inhibits myocardial fibrosis in a paracrine manner by activating thegp130/JAK2/STAT3 pathway in cardiomyocytes. [pubmed:28115285] |
Cellular physiology and biochemistry | Cholecystokinin Expression in the Development of Postinfarction Heart Failure. [pubmed:29130944] |
JOURNAL OF PHARMACEUTICAL SCIENCES | Assessment of the role of cholecystokinin in hyperemesis gravidarum and correlation with its severity [:] |
Journal of Dairy Science | Effects of dietary leucine and phenylalanine on pancreas development, enzyme activity, and relative gene expression in milk-fed Holstein dairy calves [Pubmed:29477524] |
Food and Nutrition Research | Alignments of endocrine, anthropometric, and metabolic parameters in type 2 diabetes after intervention with an Okinawa-based Nordic diet [Pubmed:29599686] |
International Heart Journal | The Relationship of Appetite-Regulating Hormones in the Development of Cardiac Cachexia [Pubmed: 30799377] |
Chinese Medicine | Hejie Zhitong prescription promotes sleep and inhibits nociceptive transmission-associated neurotransmitter activity in a rodent migraine model [Pubmed: 33014123] |
foods | Appetite and Satiety Effects of the Acute and Regular Consumption of Green Coffee Phenols and Green Coffee Phenol/Oat β-Glucan Nutraceuticals in Subjects with … [34828792] |
npj Biofilms and Microbiomes | The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal [Pubmed:35379849] |
Food Funct | Digestion characteristics of quinoa, barley and mungbean proteins and the effects of their simulated gastrointestinal digests on CCK secretion in enteroendocrine STC … [Pubmed:35587126] |