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Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Cross Linked C-Telopeptide Of Type I Collagen (CTXI) ,etc. by FLIA (Flow Luminescence Immunoassay)

CTX-I; CTX1;

（注：单次混测多因子不超过8个指标 ）

  • Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 93-103 99
EDTA plasma(n=5) 94-104 101
heparin plasma(n=5) 80-102 80
sodium citrate plasma(n=5) 80-93 85

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 78-89% 84-103% 95-102% 92-101%
EDTA plasma(n=5) 87-96% 95-105% 98-105% 79-104%
heparin plasma(n=5) 95-103% 87-96% 97-105% 90-103%
sodium citrate plasma(n=5) 83-90% 90-97% 79-92% 84-104%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
    37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法)和未标记的Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法)呈负相关。

赠品

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
CEA665Ra Ⅰ型胶原交联羧基端肽(CTXI)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA665Ra Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA665Ra11 Ⅰ型胶原交联羧基端肽(CTXI)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
journal of bone and mineral research Atp6v0d2 Is an Essential Component of the Osteoclast-Specific Proton Pump That Mediates Extracellular Acidification in Bone Resorption [PubMed: PMC2672205]
Bone Borage and fish oils lifelong supplementation decreases inflammation and improves bone health in a murine model of senile osteoporosis [PubMed: 21664309]
Trials Is there benefit in optimising heart failure treatment in over-80 year-old patients? (HF-80 study): study protocol for a randomized controlled trial. [Trials: 1745-6215]
Journal of Biological Chemistry TRAF family member-associated NF-κB activator (TANK) is a negative regulator of osteoclastogenesis and bone formation [PubMed: 22773835]
The Journal of Clinical Endocrinology & Metabolism A Mediterranean Diet Enriched with Olive Oil Is Associated with Higher Serum Total Osteocalcin Levels in Elderly Men at High Cardiovascular Risk [PubMed: PMC3462931]
Endocrinology Oxytocin Reverses Ovariectomy-Induced Osteopenia and Body Fat Gain [Endocrine: Source]
Am J Physiol Endocrinol Metab. DPP IV inhibitor treatment attenuates bone loss and improves mechanical bone strength in male diabetic rats [Pubmed:25053403]
The American Journal of Cardiology Relation of burden of myocardial fibrosis to malignant ventricular arrhythmias and outcomes in Fabry disease [Pubmed:25073565]
Rheumatology (Oxford). Bone resorption correlates with the frequency of CD5+ B cells in the blood of patients with rheumatoid arthritis [Pubmed:25193807]
XPO1/CRM1-Selective Inhibitors of Nuclear Export (SINE) reduce tumor spreading and improve overall survival in preclinical models of prostate cancer (PCa) [Pubmed:25284315]
Rheumatology International The presence of FCGR2B promoter or transmembrane region variant alleles leads to reduced serum IL-6 levels in rheumatoid arthritis [Pubmed:25630523]
The Journal of Biological Chemistry 5-azacytidine-induced protein 2 (AZI2) regulates bone mass by fine-tuning osteoclast survival [Pubmed:25691576]
Scandinavian Journal of Clinical and Laboratory Investigation Osteocartilaginous metabolic markers change over a 3-week stage race in pro-cyclists [PubMed: 26174975]
AIDS Effects of renal tubular dysfunction on bone in tenofovir-exposed HIV-positive patients [PubMed: 26372384]
the journal of rheumatology Biomarkers in Remission According to Different Criteria in Patients with Rheumatoid Arthritis [PubMed: 26472417]
Int J Mol Med Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway [PubMed: 26677102]
the journal of biological chemistry Mutation in Osteoactivin Promotes RANKL-Mediated Osteoclast Differentiation and Survival, but Inhibits Osteoclast Function [PubMed: 25837253]
frontiers in endocrinology Oxytocin reverses osteoporosis in a sex-dependent manner [PubMed: 26042090]
PLoS One.  The RIP1–RIP3 Complex Mediates Osteocyte Necroptosis after Ovariectomy in Rats [pmc:PMC4795547]
J Cell Physiol Lanthanum Chloride Attenuates Osteoclast Formation and Function Via the DownreCavia (Guinea pig )lation of Rankl-Induced Nf-κb and Nfatc1 Activities [Pubmed:26060084]
FASEB Journal N-Homocysteinylation impairs collagen cross-linking in cystathionine β-synthase-deficient mice: a novel mechanism of connective tissue abnormalities. [pubmed:27530978]
Metabolism. High FGF21 levels are associated with altered bone homeostasis in HIV-1-infected patients. [pubmed:28521869]
journal of science and medicine in sport The effect of calcium and vitamin D supplementation on bone health of male Jockeys. [pubmed:27568072]
Chemico-Biological Interactions Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway [pubmed:27387537]
Epilepsy Research The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats [pubmed:27838501]
Bone Reduced femoral bone mass in both diet-induced and genetic hyperlipidemia mice [pubmed:27669658]
Journal of Vascular Surgery A potential role for glycated cross-links in abdominal aortic aneurysm disease. [pubmed:27624703]
Institute of Pharmacology Effects of long-term administration of pantoprazole on bone mineral density in young male rats. [pubmed:27552060]
Biomedicine & Pharmacotherapy Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats [S0753332216322752]
European Journal of Pharmacology Cannabidiol administration reduces sublesional cancellous bone loss in rats with severe spinal cord injury [pubmed:28479140]
APPLIED SPECTROSCOPY Side-Effects of Convulsive Seizures and Anti-Seizure Therapy on Bone in a Rat Model of Epilepsy [pubmed:28905646]
Clinical Nutrition Obesity status influences the relationship among serum osteocalcin, iron stores and insulin sensitivity [pubmed:29050649]
Oncotarget A novel osteoporosis model with ascorbic acid deficiency in Akr1A1 gene knockout mice. [pubmed:28060768]
中国骨质疏松杂志 骨形成指标PINP 与骨吸收指标β-CTX 在2 型糖尿病合并骨质疏松中作用的研究. [10.3969/j.issn.1006-7108.2017.03.009]
BioMed Research International Optimization of the Time Window of Interest in Ovariectomized Imprinting Control Region Mice for Antiosteoporosis Research [pubmed:29119115]
Cell Death And Differentiation TSC1 regulates osteoclast podosome organization and bone resorption through mTORC1 and Rac1/Cdc42 [Pubmed:29358671]
Journal of Periodontology  The role of androgens on periodontal repair in female rats [Pubmed:29683499]
Life Sciences Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathway [Pubmed:29883721]
European Journal of Pharmacology The effect of levetiracetam on rat bone mineral density, bone structure and biochemical markers of bone metabolism [Pubmed:29428468]
Journal of Clinical Densitometry Geometric and mechanical bone response to a multidisciplinary weight loss intervention in adolescents with obesity: The ADIBOX study [Pubmed:30076009]
Pharmacology Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats [Pubmed:29898457]
Experimental and Therapeutic Medicine Liraglutide exerts a bone‑protective effect in ovariectomized rats with streptozotocin‑induced diabetes by inhibiting osteoclastogenesis [Pubmed:29805533]
Experimental and Therapeutic Medicine Combination therapy with BMP‑2 and psoralen enhances fracture healing in ovariectomized mice [10.3892:etm.2018.6353]
Tumor Biology Dual PI3 K/mTOR inhibition reduces prostate cancer bone engraftment altering tumor-induced bone remodeling [Pubmed:29687745]
Sensors Membrane-Based Microwave-Mediated Electrochemical Immunoassay for the In Vitro, Highly Sensitive Detection of Osteoporosis-Related Biomarkers [Pubmed: 30181433]
Tropical Journal of Pharmaceutical Research Mollugin attenuates glucocorticoid-induced osteoporosis in rats via Akt/P13K pathway []
Bone & Joint Research Transcriptome analysis of osteoblasts in an ovariectomized mouse model in response to physical exercise []
Toxicol Ind Health Influence of physical training on markers of bone turnover, mechanical properties, morphological alterations, density and mineral contents in the femur of rats exposed … [Pubmed: 30983556]
Journal of Geriatric Oncology Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors [Pubmed: 30952517]
Scientific Reports Significance of the Tks4 scaffold protein in bone tissue homeostasis []
E The Effects of Ficus Carica Fruit on Bone Markers and Oestrogen Level of Post-Menopausal Osteoporotic Rats []
Biology Open Osteocyte TSC1 promotes sclerostin secretion to restrain osteogenesis in mice [Pubmed: 31088250]
Journal of Periodontology Role of testosterone and androgen receptor in periodontal disease progression in female rats [Pubmed: 31389012]
Journal of clinical periodontology Biomarkers of oral inflammation in perinatally HIV‐infected and perinatally HIV exposed, uninfected youth [Pubmed: 31385616]
Bulletin of Experimental Biology and Medicine Laboratory Monitoring of Bone Tissue Remodeling after Augmentation of Impression Intraarticular Fracture with Different Types of Bone Graft [Pubmed: 31630302]
Molecular Therapy-Nucleic Acids AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activities [Pubmed: 31739209]
International Journal of Environmental Research and Public Health Alleviating Effect of α-Lipoic Acid and Magnesium on Cadmium-Induced Inflammatory Processes, Oxidative Stress and Bone Metabolism Disorders in Wistar Rats [Pubmed: 31739465]
International journal of molecular sciences Differences in Osteoimmunological Biomarkers Predictive of Psoriatic Arthritis among a Large Italian Cohort of Psoriatic Patients [Pubmed: 31717649]
Brazilian Journal of Medical and Biological Research Beneficial effects of Cuscuta chinensis extract on glucocorticoid-induced osteoporosis through modulation of RANKL/OPG signals [Pubmed: 31826180]
Journal of Bone and Mineral Research BMP9 reduces bone loss in ovariectomized mice by dual regulation of bone remodeling [Pubmed: 31914211]
Theranostics EDTA-Modified 17β-Estradiol-Laden Upconversion Nanocomposite for Bone-Targeted Hormone Replacement Therapy for Osteoporosis [Pubmed: 32194868]
Cells Article Dual CXCR4 and E-Selectin Inhibitor, GMI-1359, Shows Anti-Bone Metastatic Effects and Synergizes with Docetaxel in Prostate Cancer Cell Intraosseous … [Pubmed: 31877673]
Cell RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis [Pubmed: 32640190]
Advanced Science A Loop‐Based and AGO‐Incorporated Virtual Screening Model Targeting AGO‐Mediated miRNA–mRNA Interactions for Drug Discovery to Rescue Bone Phenotype … [Pubmed: 32670749]
International Medical Journal Malaysia The Effects of Ficus Carica Fruit on Bone Markers and Oestrogen Level of Post-Menopausal Osteoporotic Rats. []
Cells. Dual CXCR4 and E-Selectin Inhibitor, GMI-1359, Shows Anti-Bone Metastatic Effects and Synergizes with Docetaxel in Prostate Cancer Cell Intraosseous … []
Journal of Orthopaedic Translation Qu Feng Zhi Tong capsule increases mechanical properties of cortical bone in ovariectomized rats []
Research square 4-Hexylresorcinol Inhibits Osteoclastogenesis by Suppressing NF‐κB Signaling Pathway and Reverses Bone Loss in Ovariectomized Mice []
IIUM Medical Journal Malaysia The Effects of Ficus Carica Fruit on Bone Markers and Oestrogen Level of Post-Menopausal Osteoporotic Rats []
淫羊藿水提物及提取药渣的抗骨质疏松药效比较研究 []
Pamukkale Tıp Dergisi Postmenapozal Tip-2 diyabetes mellitus' lu hastalarda osteoporoza bağlı kırık riskinin ve kemik Turnoveri'nin değerlendirilmesi []
Science Advances A biomimetically hierarchical polyetherketoneketone scaffold for osteoporotic bone repair [33310848]
J Bone Miner Res Lgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/¦Â©\catenin pathway [33950533]
Br J Pharmacol Andrographolide prevents bone loss via targeting ERRalpha‐regulated metabolic adaption of osteoclastogenesis [34233019]
PHARMACEUTICAL BIOLOGY Effects of icariin on the fracture healing in young and old rats and its mechanism [34511043]
Evid Based Complement Alternat Med Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway [34512780]
Scandinavian Cardiovascular Journal Dual integrin αvβ3 and αvβ5 blockade attenuates cardiac dysfunction by reducing fibrosis in a rat model of doxorubicin-induced cardiomyopathy [34296634]
Chronobiol Int Night shift work and osteoporosis-bone turnover markers among female blue-collar workers in Poland [Pubmed:35139709]
Cell Metab Defects in a liver-bone axis contribute to hepatic osteodystrophy disease progression [Pubmed:35235775]
Cell Death Discov BMP9 reduces age-related bone loss in mice by inhibiting osteoblast senescence through Smad1-Stat1-P21 axis [Pubmed:35523787]
Experimental and Molecular Medicine Foxf2 represses bone formation via Wnt2b/β-catenin signaling [Pubmed:35668101]
Bioactive Materials Osteoporotic bone recovery by a bamboo-structured bioceramic with controlled release of hydroxyapatite nanoparticles [Pubmed:35386445]
Journal of Orthopaedic Translation Puerarin specifically disrupts osteoclast activation via blocking integrin-β3 Pyk2/Src/Cbl signaling pathway [Pubmed:35228997]
Journal of Orthopaedic Translation Exercised accelerated the production of muscle-derived kynurenic acid in skeletal muscle and alleviated the postmenopausal osteoporosis through the Gpr35 … []
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