太阳集团tyc5997_平台(china)官方网站

铁蛋白(FE)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Ferritin (FE) ,etc. by FLIA (Flow Luminescence Immunoassay)

（注：单次混测多因子不超过8个指标 ）

  • 铁蛋白(FE)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 铁蛋白(FE)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测铁蛋白(FE)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的铁蛋白(FE)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 94-101 97
EDTA plasma(n=5) 97-105 102
heparin plasma(n=5) 78-101 89

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的铁蛋白(FE)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中铁蛋白(FE)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 80-102% 87-105% 88-98%
EDTA plasma(n=5) 89-98% 95-104% 78-105% 96-103%
heparin plasma(n=5) 93-102% 91-98% 87-97% 84-92%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
    37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将铁蛋白(FE)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的铁蛋白(FE)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的铁蛋白(FE)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的铁蛋白(FE)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
PAA518Ra01 铁蛋白(FE)多克隆抗体 WB; IHC; ICC; IP.
WEA518Ra 铁蛋白(FE)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA518Ra 铁蛋白(FE)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA518Ra 铁蛋白(FE)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Molecular Endocrinology PGC-1α regulates hepatic hepcidin expression and iron homeostasis in response to inflammation. [Pubmed: 23438894]
experimental oncology Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma. [PubMed: 25804229]
J Cancer Sci Ther Host H68D Genotype Affects Tumor Growth in Mouse Melanoma [Open-Access: 1948-5956-1000353]
Food Funct The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice [Pubmed:26757699]
Physiological research The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Disease [pubmed:27782743]
Expert Review of Hematology 4 (th) Mediterranean Multidisciplinary Course on Iron Anemia April 29 (th)-30 (th) 2016, Madrid, Spain. [pubmed:27615544]
Oxidative Medicine and Cellular Longevity Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation [pubmed:29147463]
European Journal of Pharmaceutical Sciences sucrosomial® iron absorption studied by in vitro and ex-vivo models [pubmed:29055735]
Journal of Food Science and Technology In vitro assessment of bio-augmented minerals from peanut oil cakes fermented by Aspergillus oryzae through Caco-2 cells. [pubmed:29051659]
International Journal of Cuban Health & Medicine Anemia and Iron Deficiency Related to Inflammation, Helicobacter pylori Infection and Adiposity in Reproductive-age Cuban Women [10.1590/MEDICC.2017.1902030004 ]
Oman Medical Journal The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus. [pubmed:28584599]
Food Bioscience Preparation of lactose-iron complex and its cyto-toxicity, in-vitro digestion and bioaccessibility in Caco-2 cell model system [10.1016/j.fbio.2017.10.001]
Nutrients. Long-Term Dexamethasone Exposure Down-Regulates Hepatic TFR1 and Reduces Liver Iron Concentration in Rats [pubmed:28629118]
Food Chemistry Physicochemical characterization of mineral (iron/zinc) bound caseinate and their mineral uptake in Caco-2 cells [Pubmed:29622185]
Animal Feed Science and Technology Super-dosing phytase improves the growth performance of weaner pigs fed a low iron diet [10.1016:j.anifeedsci.2018.01.012]
American Journal of Hematology Iron homeostasis in pregnancy and spontaneous abortion [Pubmed: 30394565]
The Nigerian postgraduate medical journal the official publication of the National Postgraduate Medical College of Nigeria Assessment of iron deficiency anaemia and its risk factors among adults with chronic kidney disease in a tertiary hospital in Nigeria []
Haematologica New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia [Pubmed: 30792208]
博士论文 PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE []
Synthesis, characterization and cellular mineral absorption of nanoemulsions of Rhododendron arboreum flower extracts stabilized with gum arabic []
Cell Research Transferrin plays a central role in coagulation balance by interacting with clotting factors [Pubmed: 31811276]
international journal of biological macromolecules Gum arabic capped copper nanoparticles: Synthesis, characterization, and applications [Pubmed: 31904465]
journal of the american college of nutrition Improvement of Mineral Absorption and Nutritional Properties of Citrullus vulgaris Seeds Using Solid-State Fermentation [Pubmed: 32255407]
LWT-FOOD SCIENCE AND TECHNOLOGY Whey protein-iron or zinc complexation decreases pro-oxidant activity of iron and increases iron and zinc bioavailability []
J Mol Cell Cardiol Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure [34139233]
European Journal of Pharmacology The mechanism of the imbalance between proliferation and ferroptosis in pulmonary artery smooth muscle cells based on the activation of SLC7A11 [Pubmed:35700835]
留言咨询
Baidu
sogou