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半乳糖凝集素3(GAL3)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Galectin 3 (GAL3) ,etc. by FLIA (Flow Luminescence Immunoassay)

LGALS3; CBP35; GALBP; GALIG; MAC2; 35 kDa lectin; Carbohydrate-binding protein 35; Galactose-specific lectin 3; Galactoside-binding protein; Laminin-binding protein; Lectin L-29

（注：单次混测多因子不超过8个指标 ）

  • 半乳糖凝集素3(GAL3)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 半乳糖凝集素3(GAL3)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测半乳糖凝集素3(GAL3)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的半乳糖凝集素3(GAL3)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 78-104 94
EDTA plasma(n=5) 96-103 99
heparin plasma(n=5) 84-95 90

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的半乳糖凝集素3(GAL3)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中半乳糖凝集素3(GAL3)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 82-104% 89-97% 85-99% 82-97%
EDTA plasma(n=5) 88-96% 90-105% 86-101% 81-90%
heparin plasma(n=5) 99-105% 94-101% 88-97% 95-105%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
    37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将半乳糖凝集素3(GAL3)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的半乳糖凝集素3(GAL3)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的半乳糖凝集素3(GAL3)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的半乳糖凝集素3(GAL3)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
APA303Ra01 半乳糖凝集素3(GAL3)活性蛋白 Cell culture; Activity Assays.
RPA303Ra01 半乳糖凝集素3(GAL3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA303Ra01 半乳糖凝集素3(GAL3)多克隆抗体 WB; IHC; ICC; IP.
LAA303Ra81 半乳糖凝集素3(GAL3)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA303Ra71 半乳糖凝集素3(GAL3)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA303Ra24 半乳糖凝集素3(GAL3)单克隆抗体 WB; IHC; ICC; IP.
MAA303Ra23 半乳糖凝集素3(GAL3)单克隆抗体 WB; IHC; ICC; IP.
MAA303Ra21 半乳糖凝集素3(GAL3)单克隆抗体 WB; IHC; ICC; IP.
MAA303Ra22 半乳糖凝集素3(GAL3)单克隆抗体 WB; IHC; ICC; IP.
SEA303Ra 半乳糖凝集素3(GAL3)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA303Ra 半乳糖凝集素3(GAL3)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
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Scientifi Reportcs Galectin-3 level and the severity of cardiac diastolic dysfunction using cellular and animal models and clinical indices [PubMed: 26582585]
Clin Chim Acta Galectin-1 and galectin-4: Plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population [PubMed: 25578396]
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Cardiology Galectin-3 Induces Atrial Fibrosis by Activating the TGF-β1/Smad Pathway in Patients with Atrial Fibrillation [Pubmed: 32516780]
AUSTRALIAN DENTAL JOURNAL The effect of initial periodontal treatment on gingival crevicular fluid galectin©\3 levels in participants with periodontal disease [33378559]
Cell Death & Disease Trajectory modeling of endothelial-to-mesenchymal transition reveals galectin-3 as a mediator in pulmonary fibrosis [33771973]
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Phytomedicine Zerumbone, a humulane sesquiterpene from Syringa pinnatifolia, attenuates cardiac fibrosis by inhibiting of the TGF-β1/Smad signaling pathway after myocardial … [Pubmed:35405613]
Medicine Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive … [Pubmed:35687771]
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