饥饿素(GHRL)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Ghrelin (GHRL) ,etc. by FLIA (Flow Luminescence Immunoassay)
MTLRP; Growth Hormone-Releasing Peptide
（注：单次混测多因子不超过8个指标 ）
- 编号LMA991Bo
- 物种Bos taurus; Bovine (Cattle，牛) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长1.5h
- 检测范围9.77-10000pg/mL
- 灵敏度最小可检测剂量小于等于3.257 pg/mL.
- 样本类型Serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 8指标数 7指标数 6指标数 5指标数 4指标数 3指标数 2指标数1指标数
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测饥饿素(GHRL)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的饥饿素(GHRL)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 96-104 | 99 |
| EDTA plasma(n=5) | 82-92 | 87 |
| heparin plasma(n=5) | 83-101 | 92 |
| sodium citrate plasma(n=5) | 90-97 | 94 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的饥饿素(GHRL)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中饥饿素(GHRL)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-102% | 80-104% | 96-105% | 78-94% |
| EDTA plasma(n=5) | 97-104% | 93-101% | 82-101% | 85-95% |
| heparin plasma(n=5) | 78-98% | 80-98% | 99-105% | 80-101% |
| sodium citrate plasma(n=5) | 82-98% | 81-98% | 94-101% | 78-96% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将饥饿素(GHRL)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的饥饿素(GHRL)等多因子检测试剂盒(流式荧光发光法)和未标记的饥饿素(GHRL)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的饥饿素(GHRL)等多因子检测试剂盒(流式荧光发光法)呈负相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Bos taurus; Bovine (Cattle，牛) | 应用(仅供研究使用，不用于临床诊断！) |
| CEA991Bo | 饥饿素(GHRL)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA991Bo | 饥饿素(GHRL)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Archives of Oral Biology | Expression of ghrelin in human salivary glands and its levels in saliva and serum in Chinese obese children and adolescents [PubMed: 21075359] |
| Department of Medical Biochemistry and Clinical Biochemistry | Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cows [ScienceDirect: S0196978113000533] |
| Journal of Physiology and Pharmacology | Postnatal administration of 2-oxoglutaric acid improves articular and growth plate cartilages and bone tissue morphology in pigs prenatally treated with dexamethasone [PubMed: 23211309] |
| Gene | The modifying effects of fish oil on fasting ghrelin mRNA expression in weaned rats [PubMed: 22842192] |
| Hormone Research in Paediatrics | Changes in Ghrelin and Obestatin Levels before and after a Meal in Children with Simple Obesity and Anorexia [Pubmed: 23774845] |
| Surgical Innovation | Gastric supply manipulation to modulate ghrelin production and enhance vascularization to the cardia: proof of the concept in a porcine model [Pubmed:25294792] |
| Obesity Surgery | Embolization of Arterial Gastric Supply in Obesity (EMBARGO): an Endovascular Approach in the Management of Morbid Obesity. Proof of the Concept in the Porcine Model [Pubmed:25511751] |
| Obes Surg | Effect of Sleeve Gastrectomy Plus Side-to-Side Jejunoileal Anastomosis for Type 2 Diabetes Control in an Obese Rat Model [PubMed: 26202420] |
| Surg Innov | Gastric Supply Manipulation to Modulate Ghrelin Production and Enhance Vascularization to the Cardia Proof of the Concept in a Porcine Model [PubMed: 25294792] |
| Nutrients. | Elevation of Fasting Ghrelin in Healthy Human Subjects Consuming a High-Salt Diet: A Novel Mechanism of Obesity? [Pubmed:27240398] |
| World Journal of Gastroenterology | Effects of sleeve gastrectomy with jejuno-jejunal or jejuno-ileal loop onglycolipid metabolism in diabetic rats. [pubmed:27621579] |
| Ginekologia Polska | Increased serum ghrelin in preeclampsia: is ghrelin a friend or a foe? [pubmed:27321099] |
| Gynecological Endocrinology | The association between serum ghrelin levels and large-for-gestational-age fetuses in patients with gestational diabetes mellitus [pubmed:27791436] |
| Acta Diabetologica | Effect of lifestyle improvement program on the biomarkersof adiposity, inflammation and gut hormones in overweight/obese Asian Indians with prediabetes [pubmed:28620678] |
| Scandinavian Journal of Clinical and Laboratory Investigation | Role of serotonin hormone in weight regain after sleeve gastrectomy [pubmed:29228802] |
| Slovenian Veterinary Research | EFFECT OF Garcinia cambogia EXTRACT ON SERUM LEPTIN, GHRELIN, ADIPONECTIN AND INSULIN LEVELS AND BODY WEIGHTS IN RATS FED WITH HIGH … [Slovetres:Source] |
| The American Journal of the Medical Sciences | The Relations Among Serum Ghrelin, Motilin and Gastric Emptying and Autonomic Function in Autoimmune Gastritis [Pubmed:29753372] |
| American Journal of Perinatology | The Role of Umbilical Cord Blood Concentration of IGF-I, IGF-II, Leptin, Adiponectin, Ghrelin, Resistin, and Visfatin in Fetal Growth [Pubmed: 30282106] |
| Stem Cells and Development | The reparative effects of human adipose-derived mesenchymal stem cells in the chemotherapy damaged thymus [Pubmed: 30511904] |
| The Journal of Nutrition | Grape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned Pigs [Pubmed: 31175811] |
| Arch Physiol Biochem | Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise training [Pubmed: 31290696] |
| Mol Med Rep | Ghrelin system is involved in improvements in glucose metabolism mediated by hyperbaric oxygen treatment in a streptozotocin‑induced type 1 diabetes mouse … [Pubmed: 32901885] |
| endocrine | Omega-3 offers better hypothalamus protection by decreasing POMC expression and elevating ghrelin hormone: a prospective trial to overcome methotrexate … [Pubmed: 32424681] |
| plos one | Serum leptin level is associated with phase angle in CKD5 patients not undergoing dialysis [Pubmed: 30089154] |
| Physiological and Histopathological Effects of Electroporation Pulse on Stomach of Rats [] | |
| International Journal of Livestock Research | Effect of Nutritional Supplementation on Treatment of Anoestrus Swamp Buffalo Cows and Heifers [] |
| iraqi journal of science | Relationship Between Orexin-A and Insulin Resistance in Patients with Type 2 Diabetes Mellitus [] |
| Indian Journal of Forensic Medicine & Toxicology | Altered Serum Marker of Adipokines Profile in Breast Cancer Women [] |
| Br J Nutr | The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study [34348809] |
| Nutrients | Nutritional Status, Selected Nutrients Intake and Their Relationship with the Concentration of Ghrelin and Adiponectin in Patients with Diabetic Nephropathy [34959967] |
| scientific reports | Effect of Sleeve Gastrectomy, Roux-En-Y Gastric Bypass, and Ileal Transposition on Myocardial Ischaemia–Reperfusion Injury in Non-Obese Non-Diabetic Rats [34903800] |
| npj Biofilms and Microbiomes | The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal [Pubmed:35379849] |
| Food Hydrocolloids | Creating texturally tuneable, low calorie and palatable noodle-like food assemblies via microfluidics [] |