谷胱甘肽过氧化酶1(GPX1)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Glutathione Peroxidase 1 (GPX1) ,etc. by FLIA (Flow Luminescence Immunoassay)
GSHPX1; Cellular glutathione peroxidase
（注：单次混测多因子不超过8个指标 ）
- 编号LMA295Po
- 物种Sus scrofa; Porcine (Pig，猪) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围0.1-100ng/mL
- 灵敏度最小可检测剂量小于等于0.033 ng/mL.
- 样本类型Serum, plasma, tissue homogenates and other biological fluids
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测谷胱甘肽过氧化酶1(GPX1)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的谷胱甘肽过氧化酶1(GPX1)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 81-98 | 85 |
| EDTA plasma(n=5) | 81-96 | 92 |
| heparin plasma(n=5) | 78-101 | 84 |
| sodium citrate plasma(n=5) | 98-105 | 102 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的谷胱甘肽过氧化酶1(GPX1)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中谷胱甘肽过氧化酶1(GPX1)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-94% | 98-105% | 95-102% | 78-93% |
| EDTA plasma(n=5) | 78-91% | 83-101% | 87-101% | 85-102% |
| heparin plasma(n=5) | 91-99% | 78-92% | 97-104% | 83-101% |
| sodium citrate plasma(n=5) | 80-91% | 96-104% | 84-95% | 99-105% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将谷胱甘肽过氧化酶1(GPX1)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的谷胱甘肽过氧化酶1(GPX1)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的谷胱甘肽过氧化酶1(GPX1)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的谷胱甘肽过氧化酶1(GPX1)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Sus scrofa; Porcine (Pig，猪) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA295Po01 | 谷胱甘肽过氧化酶1(GPX1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA295Po01 | 谷胱甘肽过氧化酶1(GPX1)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA295Po21 | 谷胱甘肽过氧化酶1(GPX1)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA295Po | 谷胱甘肽过氧化酶1(GPX1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA295Po | 谷胱甘肽过氧化酶1(GPX1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| PLoS One. | P2×7?Receptor in the Kidneys of Diabetic Rats Submitted to Aerobic Training or to N-Acetylcysteine Supplementation [Plos:Source] |
| AGE | Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closure [Pubmed:24443098] |
| Anticancer Research | Determination of Gene Expression and Serum Levels of MnSOD and GPX1 in Colorectal Cancer [Pubmed:25550558] |
| Adv Med Sci | Genetic polymorphisms (Pro197Leu of Gpx1, +35A/C of SOD1, [PubMed: 26674569] |
| Applied Biological Research | Seasonal Variation in Glutathione Peroxidase in Seminal Plasma of Karan Fries (Tharparkar X Holstein Friesian) Bulls Under Tropical Climatic Conditions [publication:301246301] |
| Cell Biol Int | Influence of season on seminal antioxidant enzymes in Karan Fries bulls under tropical climatic conditions [fileID:697741] |
| Toxicology Mechanisms and Methods | Evaluation of hepatorenal impairments in Wistar rats coexposed to low-dose lead, cadmium and manganese: insights into oxidative stress mechanism [pubmed:27599793] |
| Journal of Environmental Research and Public Health | Reduced Dietary Selenium Impairs Vascular Function by Increasing Oxidative Stress in Sprague-Dawley Rat Aortas. [pubmed:28574428] |
| Pharmacological Reports | Assessing the serum concentration levels of NT-4/5, GPX-1, TNF-α, and l-arginine as biomediators of depression severity in first depressive episode patients with and without posttraumatic stress disorder. [pubmed:28958613] |
| CNS Spectrums | Peripheral levels of superoxide dismutase and glutathione peroxidase in youths in ultra-high risk for psychosis: a pilot study [pubmed:29248027] |
| Environmental toxicology and pharmacology | Dyslipdemia induced by chronic low dose co-exposure to lead, cadmium and manganese in rats: the role of oxidative stress [pubmed:28654832] |
| REVISTA DE CHIMIE | Selenium- Essential Antioxidant Elemen the example of autoimune thyroiditis [pdf:44%20PREDA%20C%207%2017.pdf] |
| Biological Rhythm Research | Seasonal variation of mitochondria activity related and heat shock protein genes in spermatozoa of Karan Fries bulls in tropical climate [10.1080:09291016.2017.1361584] |
| Investigation of GPX1 Gene Expression, Serum GPX1 and Selenium Levels on Colorectal Cancer [] | |
| Kolon Kanserinde Gpx1 Gen Anlatımı, Serum Gpx1 ve Selenyum Düzeylerinin İncelenmesi [] | |
| Alcohol. | Influence of deficient nutrition on trace element status and antioxidant defense system [Pubmed: 1781925] |
| BMC Infectious Diseases | Glutathione, glutathione peroxidase and some hematological parameters of HIV-seropositive subjects attending clinic in University of Calabar teaching … [Pubmed: 31703562] |
| LIFE SCIENCES | Rapamycin improves renal injury induced by Iodixanol in diabetic rats by deactivating the mTOR/p70S6K signaling pathway [Pubmed: 32798557] |
| AMELIORATIVE EFFECT OF AQUEOUS EXTRACT OF HIBISCUS SABDARIFFA (ROSELLE) ON SALT-INDUCED HYPERTENSION IN WISTAR RATS [] | |
| The Journal of Physiology | In Vitro Cell Culture Study of Anticholesterol and Antioxidant Activities of Myrmecodia platytyrae (MyP) Extract in WRL-68 Cells [] |
| World Neurosurg | Effects of Ganoderma lucidum polysaccharides on different pathways involved in the development of spinal cord ischemia reperfusion injury: biochemical ¡ [33689849] |
| J Pers Med | Associations of Antioxidant Enzymes with the Concentration of Fatty Acids in the Blood of Men with Coronary Artery Atherosclerosis [34945751] |
| J Dairy Sci | Intake of Bifidobacterium lactis Probio-M8 fermented milk protects against alcoholic liver disease [Pubmed:35086715] |
| Biomed Res Int | A Famous Chinese Medicine Formula: Yinhuo Decoction Antagonizes the Damage of Corticosterone to PC12 Cells and Improves Depression by Regulating … [Pubmed:35281603] |