太阳集团tyc5997_平台(china)官方网站

白介素17(IL17)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Interleukin 17 (IL17) ,etc. by FLIA (Flow Luminescence Immunoassay)

IL17A; CTLA8; IL-17A; Cytotoxic T-Lymphocyte-Associated Protein 8

（注：单次混测多因子不超过8个指标 ）

  • 白介素17(IL17)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 白介素17(IL17)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测白介素17(IL17)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的白介素17(IL17)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 89-98 94
EDTA plasma(n=5) 78-99 89
heparin plasma(n=5) 96-105 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的白介素17(IL17)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中白介素17(IL17)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 88-104% 97-105% 79-90% 79-88%
EDTA plasma(n=5) 87-97% 93-101% 80-90% 84-101%
heparin plasma(n=5) 94-101% 97-105% 96-104% 89-99%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
    37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将白介素17(IL17)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的白介素17(IL17)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的白介素17(IL17)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的白介素17(IL17)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
APA063Mu61 白介素17(IL17)活性蛋白 Cell culture; Activity Assays.
RPA063Mu02 白介素17(IL17)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA063Mu01 白介素17(IL17)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA063Mu01 白介素17(IL17)多克隆抗体 WB; IHC; ICC; IP.
PAA063Mu02 白介素17(IL17)多克隆抗体 WB; IHC; ICC; IP.
MAA063Mu22 白介素17(IL17)单克隆抗体 WB; IHC; ICC; IP.
SEA063Mu 白介素17(IL17)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA063Mu 白介素17(IL17)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA063Mu 白介素17(IL17)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA063Mu 白介素17(IL17)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA063Mu 白介素17(IL17)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
PLOS ONE Impaired Autoimmune T Helper 17 Cell Responses Following DNA Vaccination against Rat Experimental Autoimmune Encephalomyelitis [PubMed: PMC2577303]
Cellular Immunology Atorvastatin ameliorates experimental autoimmune neuritis by decreased Th1/Th17 cytokines and up-regulated T regulatory cells [ScienceDirect: S0008874911002164]
The Journal of Urology Improvement by Phytotherapeutic Agent of Detrusor Overactivity, Down-Regulation of Pharmacological Receptors and Urinary Cytokines in Rats with Cyclophosphamide Induced Cystitis [PubMed: S0022534712049087]
Critical Care Medicine Systemic release of cytokines and heat shock proteins in porcine models of polytrauma and hemorrhage [PubMed: PMC3383056]
Lower Urinary Tract Symptoms Effects of Saw Palmetto Extract on Urodynamic Parameters, Bladder Muscarinic and Purinergic Receptors and Urinary Cytokines in Rats with Cyclophosphamide-Induced Cystitis [Onlinelibrary: luts.12015]
Shock Expression of Hemopexin in Acute Rejection of Rat Liver Allograft Identified by Serum Proteomic Analysis. [Lww: Source]
J Vet Med Sci. CD4+ T Cell Cytokine Gene and Protein Expression in Duodenal Mucosa of Dogs with Inflammatory Bowel Disease [Pubmed:24270804]
Journal of Dairy Science Increased blood-circulating interferon-γ, interleukin-17, and osteopontin levels in bovine paratuberculosis [Pubmed:24679935]
Inflammation.? Pemetrexed Ameliorates Experimental Arthritis in Rats [Pubmed:25129058]
Inflammation Lapatinib Ameliorates Experimental Arthritis in Rats [Pubmed:25283886]
Molecular and Cellular Biochemistry Beta-arrestin-2 negatively modulates inflammation response in mouse chondrocytes induced by 4-mer hyaluronan oligosaccharide [Pubmed:25318610]
Molecular Medicine Reports Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosis [Pubmed:25323532]
Genet Mol Res Protective effect and mechanism of probucol in the treatment of spinal cord injury [PubMed: 26214485]
veterinary research forum Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells [Article_14936_6.Html: ]
Tumor Biology Hypoxia-induced secretion of IL-20 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151]
Clinical & Experimental Immunology MicroRNA‐155 may be involved in the pathogenesis of atopic dermatitis by modulating the differentiation and function of T helper type 17 (Th18) cells [PubMed: 25761610]
J Reprod Immunol The immunomodulating effect of seminal plasma on T cells [PubMed: 25799173]
Biomarkers Interleukin-17 levels correlate with poor prognosis and vascular endothelial growth factor concentration in the serum of patients with non-small cell lung cancer [PubMed: 26301881]
Molecular Medicine Reports Tanshinone IIA attenuates experimental autoimmune encephalomyelitis in rats [Pubmed:27357729]
SpringerPlus Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression ofHMGB1/RAGE/NF-ΚB pathway. [Pubmed:27026909]
International Immunopharmacology Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery [Pubmed:26802602]
Journal of Agricultural Biotechnology Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells [10610]
Veterinary Research Communications Immunological and pathological investigations in equine experimental uveitis [pubmed:27344152]
Journal of Neuroinflammation Th17-skewed immune response and cluster of differentiation 40 ligand expression in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis. [pubmed:28114998]
American Journal of Translational Research Anti-inflammatory effects of Boletus edulis polysaccharide on asthma pathology [pubmed:27830033]
Springerplus Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression of HMGB1/RAGE/NF-ΚB pathway [pubmed:27026909]
Journal of Virology Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys. [pubmed:]
J Nephropathol Relationship between IL-17 serum level and ambulatory blood pressure in women with polycystic ovary syndrome [pubmed:28042549]
Journal of Nephropathology Relationship between IL-17 and ambulatory blood pressure in polycystic ovary syndrome [8b168a09f5832df1b5c2f48631612458b2d8.pdf]
Basic Clin Pharmacol Toxicol. Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling. [pubmed:28032440]
Gut Pathog. Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice [pubmed:28250847]
Procalcitonin und proinflammatorische Zytokine in der bronchoalveolären Lavage beim lungenkranken Pferd unter inhalativer Glukokortikoidtherapie [FUDISS_thesis_000000103830]
Scientific Report Anti-asthmatic efect of pitavastatin through aerosol inhalation is associated with CD4+ CD25+ Foxp3+ T cells in an asthma mouse model [pubmed:28729731]
Basic & Clinical Pharmacology & Toxicology Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling [doi:10.1111]
Academic Journal Antiinflammatory and antioxidant effects of gemcitabine in collagen-induced arthritis model [pubmed:28618762]
International Journal of Clinical and Experimental Pathology Low-density neutrophils in severe fever with thrombocytopenia syndrome (SFTS) display decreased function to phagocytose SFTS virus and enhanced capacity to synthesize cytokines [ISSN:1936-2625/IJCEP0045334]
Asian Pacific Society of Respirology Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice [pubmed:28122397]
MYOPAIN Increased Levels of IL-17, IL-23, MIP-1α, MCP-1 and Global Leukocytes in Fibromyalgia Patients [10.1080/24708593.2017.1357664]
Molecular Medicine Reports Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia. [pubmed:29257310]
Molecular Medicine Reports Interleukin-17 antagonist attenuates lung inflammation through inhibition of the ERK1/2 and NF-κB pathway in LPS-induced acute lung injury [10.3892/mmr.2017.6837]
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY  Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosis [pubmed:28608724]
International Immunopharmacology Therapeutic effect of bone marrow mesenchymal stem cells pretreated with acetylsalicylic acid on experimental periodontitis in rats [pubmed:29195233]
Biomedical Research Associations among interleukin-17, interferon-gamma, and acute coronary syndrome [ISSN 0970-938X]
Cellular Physiology and Biochemistry Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory … [Pubmed:29672286]
Frontiers in Pharmacology Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal … [Pubmed:29375374]
Bioscience Reports Decreased miR-128 expression alleviated the progression of Rheumatoid arthritis by up-regulating the expression of TNFAIP3 [Pubmed:29853534]
Medical Science Monitor Involvement of T-Helper 9 Activation in a Mouse Model of Allergic Rhinitis [Pubmed:29982264]
Journal of International Medical Research Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease [Pubmed:29950127]
Canadian Journal of Physiology and Pharmacology Inhibition of microRNA-155 attenuates concanavalin A-induced autoimmune hepatitis by regulating Treg/Th17 cell differentiation [Pubmed: 30290123]
BMC Microbiology Nystatin enhances the immune response against Candida albicans and protects the ultrastructure of the vaginal epithelium in a rat model of vulvovaginal … [Pubmed: 30359236]
Biology of Reproduction Recombinant adiponectin alleviates abortion in mice by regulating Th17/Treg imbalance via p38MAPK-STAT5 pathway [Pubmed: 30496353]
BMC MICROBIOLOGY Enhanced antifungal activity of bovine lactoferrin-producing probiotic Lactobacillus casei in the murine model of vulvovaginal candidiasis [Pubmed: 30621597]
CNS Neuroscience & Therapeutics Negative regulation of glial Tim‐3 inhibits the secretion of inflammatory factors and modulates microglia to antiinflammatory phenotype after experimental intracerebral?… [Pubmed: 30677253]
OPEN ACCESS MACEDONIAN JOURNAL OF MEDICAL SCIENCES Seluang Fish (Rasbora Spp.) Oil Decreases Inflammatory Cytokines Via Increasing Vitamin D Level in Systemic Lupus Erythematosus []
Frontiers in Microbiology Local probiotic Lactobacillus crispatus and Lactobacillus delbrueckii exhibit strong antifungal effects against vulvovaginal candidiasis in a rat model []
中国免疫学杂志 TRAF-6 调控TGF-β1-Smad2/Smad3 信号通路在Graves 病免疫发病机制中的作用 []
Microbial Pathogenesis Therapeutic effectiveness of type I interferon in vulvovaginal candidiasis [Pubmed: 31158491]
Inflammation Protein Kinase C Theta Inhibition Attenuates Lipopolysaccharide-Induced Acute Lung Injury through Notch Signaling Pathway via Suppressing Th17 Cell Response … [Pubmed: 31297750]
international immunopharmacology Adenovirus-mediated down-regulation of miR-21-5p alleviates experimental autoimmune uveoretinitis in mice [Pubmed: 31228813]
Untersuchung einer Th17-vermittelten Immunantwort bei der kaninen idiopathischen Epilepsie []
Free Radical Biology and Medicine Vitamin D protects against oxidative stress, inflammation and hepatorenal damage induced by acute paracetamol toxicity in rat [Pubmed: 31255668]
BioMed Research International Indoleamine 2, 3-Dioxygenase (IDO) Regulates Th17/Treg Immunity in Experimental IgA Nephropathy []
Animals Anti-Inflammatory State in Arabian Horses Introduced to the Endurance Training [Pubmed: 31462005]
World Journal of Pediatrics Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis [Pubmed: 31641939]
Biotechnology and Applied Biochemistry Epigallocatechin 3‐gallate attenuates arthritis by regulating Nrf‐2, HO‐1 and cytokine levels in an experimental arthritis model [Pubmed: 31746064]
Microbial Pathogenesis Tangeretin attenuates lipopolysaccharide-induced acute lung injury through Notch signaling pathway via suppressing Th17 cell response in mice [Pubmed: 31676364]
International Journal of Rheumatic Diseases Effects of thalidomide on Th17, Treg cells and TGF‐β1/Smad3 pathway in a mouse model of systemic sclerosis [Pubmed: 31840939]
Cytokine Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis [Pubmed: 31927460]
international urology and nephrology The therapeutic effect of dendritic cells expressing indoleamine 2, 3-dioxygenase (IDO) on an IgA nephropathy mouse model [Pubmed: 31894556]
JOURNAL OF PERIODONTAL RESEARCH Salivary Del‐1, IL‐17, and LFA‐1 levels in periodontal health and disease [Pubmed: 32153040]
journal of molecular endocrinology LncRNA Profile in Hashimoto's Thyroiditis and Potential Function of NONHSAT079547. 2 [Pubmed: 32229697]
Toxicol Appl Pharmacol Daphnetin ameliorates glucocorticoid-induced osteoporosis via activation of Wnt/GSK-3β/β-catenin signaling [Pubmed: 33171191]
CARDIOVASCULAR DRUGS AND THERAPY Rhodiola crenulata reduces ventricular arrhythmia through mitigating the activation of IL-17 and inhibiting the MAPK signaling pathway [Pubmed: 32946036]
Biomed Research International Effects of 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis [Pubmed: 32908870]
J Neurosurg Covering the proximal nerve stump with chondroitin sulfate proteoglycans prevents traumatic painful neuroma formation by blocking axon regeneration after … [Pubmed: 32470939]
JOURNAL OF MICROBIOLOGY Melatonin attenuates microbiota dysbiosis of jejunum in short-term sleep deprived mice [Pubmed: 32424577]
bioscience reports Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat [Pubmed: 32395767]
Journal of Biochemical and Molecular Toxicology Vitamin D3 and calcium cosupplementation alleviates cadmium hepatotoxicity in the rat: Enhanced antioxidative and anti‐inflammatory actions by remodeling cellular … []
Revista da Associacao Medica Brasileira A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic … []
biosciences biotechnology research asia Dietary Malted Barley Grain Improved Structure and Function of Spinal Cord of Mother Rats fed on a high cholesterol diet. []
ACS Biomaterials Science & Engineering Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction []
Effects of minocycline on neuroinflammation, gut microbiome and hippocampal neurogenesis in rats with Gulf War Illness []
Minocycline Alleviate A Rat Model Of Gulf War Illness Via Altering Gut Microbiome, Attenuating Neuroinflammation And Enhancing Hippocampal Neurogenesis []
中国应用生理学杂志 丹参对非酒精性脂肪肝Th17细胞及相关细胞因子的影响* []
Rev Assoc Med Bras A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic?¡­ [33295414]
BEHAVIOURAL BRAIN RESEARCH Minocycline alleviates Gulf War Illness rats via altering gut microbiome, attenuating neuroinflammation and enhancing hippocampal neurogenesis [34000339]
Int J Clin Pract Serum Galectin©\3 Levels in Patients with Psoriasis [34137138]
cells The Effect of the Clenbuterol¡ª¦Â2-Adrenergic Receptor Agonist on the Peripheral Blood Mononuclear Cells Proliferation, Phenotype, Functions, and Reactive Oxygen?¡­ [33920705]
Mediators of Inflammation Sparstolonin B Exerts Therapeutic Effects on Collagen-Induced Arthritis by Inhibiting the NLRP3 Inflammasome and Reducing the Activity of ¦Á1, 3 ¡­ []
CNS Neurosci Ther Alterations in the gut microbiome with hemorrhagic transformation in experimental stroke [34591349]
PLoS One Evaluation of the involvement of Th17-cells in the pathogenesis of canine spinal cord injury [34591917]
J Periodontol Cytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case‐control study [34730850]
Transl Psychiatry Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive … [34802039]
Viruses Interleukin-10-Mediated Lymphopenia Caused by Acute Infection with Foot-and-Mouth Disease Virus in Mice [34960627]
PLoS One Th17 cell-mediated immune response in a subpopulation of dogs with idiopathic epilepsy [Pubmed:35025939]
Pathogens Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus Patients [Pubmed:35056005]
Biomed Pharmacother Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axis [Pubmed:35453007]
npj Biofilms and Microbiomes The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal [Pubmed:35379849]
Expert Opinion on Therapeutic Targets Dapagliflozin mitigates ovalbumin-prompted airway inflammatory-oxidative successions and associated bronchospasm in a rat model of allergic asthma [Pubmed:35549595]
留言咨询
Baidu
sogou