太阳集团tyc5997_平台(china)官方网站

白介素2(IL2)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Interleukin 2 (IL2) ,etc. by FLIA (Flow Luminescence Immunoassay)

TCGF; Lymphokine; T-Cell Growth Factor; Aldesleukin

(注:单次混测多因子不超过8个指标 )

  • 白介素2(IL2)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 白介素2(IL2)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测白介素2(IL2)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的白介素2(IL2)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 83-94 90
EDTA plasma(n=5) 92-101 97
heparin plasma(n=5) 98-105 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的白介素2(IL2)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中白介素2(IL2)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 91-105% 84-97% 98-105% 83-98%
EDTA plasma(n=5) 80-101% 95-104% 83-94% 82-94%
heparin plasma(n=5) 87-99% 90-99% 87-101% 86-105%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠)标准品或样本100μL及磁珠10μL,
    37°C酶标板振荡器孵育90分钟;
3. 磁吸甩干,加检测溶液A100μL,37°C酶标板振荡器孵育60分钟;
4. 磁吸洗板3次;
5. 加检测溶液B100μL,37°C振动孵育30分钟;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋涡震荡2分钟后读数。

实验原理

将白介素2(IL2)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠,其中的白介素2(IL2)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合,然后加入生物素化的白介素2(IL2)等多因子检测试剂盒(流式荧光发光法)抗体,将未结合的生物素化抗体洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的白介素2(IL2)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种:Sus scrofa; Porcine (Pig,猪) 应用(仅供研究使用,不用于临床诊断!)
APA073Po01 白介素2(IL2)活性蛋白 Cell culture; Activity Assays.
APA073Po61 白介素2(IL2)活性蛋白 Cell culture; Activity Assays.
RPA073Po01 白介素2(IL2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA073Po01 白介素2(IL2)多克隆抗体 WB; IHC; ICC; IP.
MAA073Po21 白介素2(IL2)单克隆抗体 WB; IHC; ICC; IP.
MAA073Po22 白介素2(IL2)单克隆抗体 WB; IHC; ICC; IP.
MAA073Po23 白介素2(IL2)单克隆抗体 WB; IHC; ICC; IP.
MAA073Po24 白介素2(IL2)单克隆抗体 WB; IHC; ICC; IP.
SEA073Po 白介素2(IL2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA073Po 白介素2(IL2)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Journal of Medicinal Plants Research Evaluation of water plantain (Alisma canaliculatum A. Br. et Bouche) and mistletoe (Viscum album L.) effects on broiler growth performance, meat composition and serum biochemical parameters [AcadeMic: source]
Bone Synthesis and inflammatory response of a novel silk fibroin scaffold containing BMP7 adenovirus for bone regeneration [PubMed: 22796416]
Nanomedicine Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform [PubMed: 23394132]
Food and Chemical Toxicology Identification and functional characterization of a novel fungal immunomodulatory protein from Postia placenta [Pubmed:25662032]
Pharmaceutical Research Molecular Mechanisms of the Modulatory Effect of Vitamin E on Tacrolimus (FK506)-Induced Renal Injury in Rats [Openview: 6Ba34F298130121B36Fc3E227Ed416F4]
PLoS Neglected Tropical Diseases Efficacy of Albendazole-Chitosan Microsphere-based Treatment for Alveolar Echinococcosis in Mice [PubMed: 26352932]
Int J Clin Exp Med Berberine alleviates preeclampsia possibly by regulating the expression of interleukin-2/interleukin-10 and Bcl-2/Bax [PubMed: 26629148]
Clin Oral Investig Can apical periodontitis affect serum levels of CRP, IL-2, and IL-6 as well as induce pathological changes in remote organs? [PubMed: 26556576]
veterinary research forum Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells [Article_14936_6.Html: ]
Pharmaceutical Biology Molecular mechanisms of the protective role of wheat germ oil against cyclosporin A-induced hepatotoxicity in rats [PubMed: 25858514]
Immunologic Research Mesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-α and PGE10 and is crucial for liver transplant tolerance [PubMed: 25982596]
J Reprod Immunol The immunomodulating effect of seminal plasma on T cells [PubMed: 25799173]
удк Циток [Cgi-Bin: Irbis_Nbuv]
Biomark Med Clinical significance of IL-2 and IL-10 gene polymorphisms and serum levels in patients with basal-cell carcinoma [PubMed: 26609634]
Environmental Science and Pollution Research Ameliorative effect of flaxseed oil against thiacloprid-induced toxicity in rats: hematological, biochemical, and histopathological study [Pubmed:26957428]
Advances in Experimental Medicine and Biology  Antiinflammatory Effect of N-Acetylcysteine Combined with Exogenous Surfactant in Meconium-Induced Lung Injury [Pubmed:27283193]
Research in Veterinary Science Effects of feed supplementation with glycine chelate and iron sulfate on selected parameters of cell-mediated immune response in broiler chickens [Pubmed:27473977]
Biomedicine & Pharmacotherapy Investigation of the anti-glioma activity of Oviductus ranae protein hydrolysate [Pubmed:27261592]
Int J Pharm Bio Sci International Journal of Pharma and Bio Sciences ISSN [cms:4987]
Asian J Pharm Clin Res INFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATS [journals:3790]
United States Patent Application HERPES VIRUS VACCINES AND TREATMENTS [y2016:0067330.html]
BMC Veterinary Research Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis [pubmed:27553600]
Vaccine. Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J. [pubmed:28049588]
Taylor & Francis Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation [09540105.2017.1306494]
Physiological Research Lung inflammatory and oxidative alterations after exogenous surfactant therapy fortified with budesonide in rabbit model of meconium aspiration syndrome. [pubmed:28006947]
Journal of Virology Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys. [pubmed:]
 Research in Veterinary The effect of feed supplementation with zinc chelate and zinc sulphate on selected humoral and cell-mediated immune parameters and cytokine concentration in … [S0034528816303162]
Research in Veterinary Science Commercial gold nanocolloid inhibits synthesis of IL-2 and proliferation of porcine T lymphocytes [science:article]
Acta Vet Hung. Imbalance of intestinal immune function in piglets infected by porcine circovirus type 2 during the fetal period. [pubmed:28244338]
Домашня сторінка ВПЛИВ КРОВОВТРАТИ НА ДИНАМІКУ ВМІСТУ ЦИТОКІНІВ СИРОВАТКИ КРОВІ В ПЕРІОД ГОСТРОЇ РЕАКЦІЇ НА КРАНІОСКЕЛЕТНУ ТРАВМУ [83646]
Journal of Agricultural and Food Chemistry Effect of boron on thymic cytokine expression, hormone secretion, antioxidant functions, cell proliferation, and apoptosis potential via the ERK1/2 signaling pathway [pubmed:29032684]
Research in Veterinary Science The effect of feed supplementation with effective microorganisms(EM) on pro- and anti-inflammatory cytokine concentrations in pigs [pubmed:28549299]
poultry science The effect of feed supplementation with Zakarpacki zeolite (clinoptilolite) on percentages of T and B lymphocytes and cytokine concentrations in poultry [pubmed:28339915]
Journal of Animal Physiology and Animal Nutrition The effect of feed supplementation with a copper-glycine chelate and copper sulphate on selected humoral and cell-mediated immune parameters, plasma superoxide dismutase activity, ceruloplasmin and cytokine concentration in broiler chickens [doi:10.1111]
Molecular Medicine Reports Interleukin-17 antagonist attenuates lung inflammation through inhibition of the ERK1/2 and NF-κB pathway in LPS-induced acute lung injury [10.3892/mmr.2017.6837]
Veterinary Microbiology A bacterial ghost improves the immunological efficacy of a Newcastle disease virus inactivated vaccine [pubmed:28619143]
Canadian Journal of Gastroenterology and Hepatology EGCG Maintains Th1/Th2 Balance and Mitigates Ulcerative Colitis Induced by Dextran Sulfate Sodium through TLR4/MyD88/NF-ݜ傠Signaling Pathway in Rats [10.1155/2017/3057268]
Veterinary Immunology and Immunopathology Integrated effect of seasons and lactation stages on the plasma inflammatory cytokines, function and receptor expression of milk neutrophils in Sahiwal (Bos indicus) cows. [pubmed:28895861]
Biological Rhythm Research A study of altered cytokine rhythms associated with successful implantation in cows [10.1080/09291016.2017.1361159]
Journal of Pain Research Sex differences in complex regional pain syndrome type I (CRPS-I) in mice [pubmed:28831269]
Canadian Journal of Gastroenterology and Hepatology EGCG Maintains Th1/Th2 Balance and Mitigates Ulcerative Colitis Induced by Dextran Sulfate Sodium through TLR4/MyD88/NF-κB Signaling Pathway in Rats [10.1155/2017/3057268]
In Vivo Study on the Hindrance Activity of Cinnamon Extract Against Aspergillus niger in Mice [ISSN: 0974-4290]
Cancer Science Sonodynamic therapy‐assisted immunotherapy: A novel modality for cancer treatment [Pubmed:29575297]
PLoS One Cyclosporine a drug-delivery system for high-risk penetrating keratoplasty: Stabilizing the intraocular immune microenvironment [Pubmed:29734357]
Probiotics Antimicrob Proteins Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a … [Pubmed:29305686]
Microbial Pathogenesis Pathogen-dependent modulation of milk neutrophils competence, plasma inflammatory cytokines and milk quality during intramammary infection of Sahiwal (Bos … [Pubmed:29787791]
Theriogenology Effect of tropical thermal stress on peri-implantation immune responses in cows [Pubmed:29625402]
Food & Nutrition Research Immunomodulatory activity of Lactobacillus plantarum KLDS1. 0318 in cyclophosphamide-treated mice [Pubmed:30026678]
Journal of Animal Physiology and Animal Nutrition The effect of feed supplementation with a copper‐glycine chelate and copper sulphate on selected humoral and cell‐mediated immune parameters, plasma … [Pubmed:28603872]
Journal of International Medical Research Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease [Pubmed:29950127]
International Journal of Environmental Studies  Effectiveness of steamed and cooked broccoli to attenuate bone marrow injury and suppressed haemopoiesis in male rats exposed to petrol vapours [10.1080:00207233.2018.1502956]
Veterinary Immunology and Immunopathology Evaluation of T regulatory lymphocytes and serum concentration of selected cytokines in dogs with perianal tumors []
International Immunopharmacology Vitamin E inhibits cyclosporin A-induced CTGF and TIMP-1 expression by repressing ROS-mediated activation of TGF-β/Smad signaling pathway in rat liver [Pubmed: 30391882]
Journal of Veterinary Research Early cytokine response after vaccination with Coxiella burnetii phase I in an infected herd of dairy cattle []
Journal of Neuroinflammation Amlexanox attenuates experimental autoimmune encephalomyelitis by inhibiting dendritic cell maturation and reprogramming effector and regulatory T cell … [Pubmed: 30823934]
International Journal of Laboratory Hematology CD56 natural killer cells exhibit abnormal phenotype and function in severe aplastic anemia []
International Immunopharmacology Ginsenoside Rg3 improves cyclophosphamide-induced immunocompetence in Balb/c mice [Pubmed: 30974284]
中国免疫学杂志 TRAF-6 调控TGF-β1-Smad2/Smad3 信号通路在Graves 病免疫发病机制中的作用 []
Animals Anti-Inflammatory State in Arabian Horses Introduced to the Endurance Training [Pubmed: 31462005]
Journal of Medicinal Food Polysaccharide from the Seeds of Plantago asiatica L. Protect Against Lipopolysaccharide-Induced Liver Injury [Pubmed: 31560611]
international urology and nephrology The therapeutic effect of dendritic cells expressing indoleamine 2, 3-dioxygenase (IDO) on an IgA nephropathy mouse model [Pubmed: 31894556]
Transboundary and Emerging Diseases Pathogenicity of porcine reproductive and respiratory syndrome virus (ORF5 RFLP 1–7–4 viruses) in China [Pubmed: 32187856]
Nanomedicine The toxicity of alumina nanoparticles to immune system in mice [Pubmed: 32162999]
Immunopharmacol Immunotoxicol Cordycepin exhibits a suppressive effect on T cells through inhibiting TCR signaling cascade in CFA-induced inflammation mice model [Pubmed: 32105161]
Journal of Receptors and Signal Transduction Research Suppressor of ras val-2 promotes inflammation-mediated oxidative stress and cell apoptosis in cardiomyocytes through activating Mst1-mROS signaling pathway [Pubmed: 32065019]
Antibiotics Effects of Early Intervention with Antibiotics and Maternal Fecal Microbiota on Transcriptomic Profiling Ileal Mucusa in Neonatal Pigs [Pubmed: 31963653]
Bone Research Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis [Pubmed: 32047705]
JOURNAL OF CHEMOTHERAPY Active metronomic vinorelbine schedules decrease plasma interleukin-2 levels in mice with Lewis lung carcinoma [Pubmed: 32930084]
THERIOGENOLOGY Porcine soluble CD83 alleviates LPS-induced abortion in mice by promoting Th2 cytokine production, Treg cell generation and trophoblast invasion [Pubmed: 32810792]
Theriogenology A comparative study on various immunological parameters influencing embryo survivability in crossbred dairy cows [Pubmed: 32810791]
Life Sci Impact of diminazene aceturate on renin-angiotensin system, infectious myocarditis and skeletal myositis in mice: An in vitro and in vivo study [Pubmed: 32652140]
BIOLOGICAL TRACE ELEMENT RESEARCH Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle [Pubmed: 32583225]
IMMUNOSTIMULATORY EFFECT OF MORINGA OLEIFERA LEAVES EXTRACT ON CYCLOPHOSPHAMIDE-INDUCED IMMUNOSUPPRESSION IN MICE []
ACS Biomaterials Science & Engineering Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction []
Immunomodulatory effects of sCD83 via induction of TGF-β1 and IDO: an in vitro and rat orthotopic liver transplantation study []
Hormonal and Cytokine Blood Profile of Dairy Cows in the Early Gestation Period []
THE CHANGE OF INDICATORS OF PROTEIN, LIPID AND CARBOHYDRATE METABOLISM IN DAIRY COWS AFTER CALVING DURING THE FORMATION OF … []
Animal Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves [33610512]
Eur Rev Med Pharmacol Sci Salivary Irisin: potential inflammatory biomarker in recurrent apthous stomatitis patients [33755963]
Dig Dis Sci Orexin A Suppresses the Expression of Exosomal PD-L1 in Colon Cancer and Promotes T Cell Activity by Inhibiting JAK2/STAT3 Signaling Pathway [34097168]
How Does the Th1/Th2 Polarization in Milk Change According to the Types of Bacteria that Cause Subclinical Mastitis in Cows? []
World J Tradit Chin Med Experimental autoimmune encephalomyelitis inhibited by Huangqi Guizhi Wuwu Decoction via Th2 cytokine enhancement []
Journal of Herbs, Spices & Medicinal Plants Immunostimulatory Effect of Moringa oleifera Extracts on Cyclophosphamide-induced Immunosuppressed Mice []
Am J Transl Res Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma [34377237]
J Immunol Res Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus [34395633]
Experimental and Therapeutic Medicine Autonomic regulation of imbalance‑induced myocardial fibrosis and its mechanism in rats with cirrhosis [34373726]
Viruses Interleukin-10-Mediated Lymphopenia Caused by Acute Infection with Foot-and-Mouth Disease Virus in Mice [34960627]
Animal Nutrition Optimizing the growth and immune system of dairy calves by subdividing the pre-weaning period and providing different milk volumes for each stage [34786502]
Cancer Commun (Lond) Anti‐PD‐L1/TGF‐βR fusion protein (SHR‐1701) overcomes disrupted lymphocyte recovery‐induced resistance to PD‐1/PD‐L1 inhibitors in lung cancer [Pubmed:34981670]
Int J Mol Sci NLRP3 Inflammasome Activation Controls Vascular Smooth Muscle Cells Phenotypic Switch in Atherosclerosis [Pubmed:35008765]
Scientific Reports A randomized controlled trial evaluating the effect of two low-level laser irradiation protocols on the rate of canine retraction [Pubmed:35710926]
Experimental and Therapeutic Medicine Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis [Pubmed:35126715]
Toxicology Research Alleviation of chemotherapy-induced acute lung injury via NLRP3/ASC/Caspase-1 signaling pathway [Pubmed:35782645]
Vaccines Age-Dependent Dynamics of Maternally Derived Antibodies (MDAs) and Understanding MDA-Mediated Immune Tolerance in Foot-and-Mouth Disease-Vaccinated … [Pubmed:35632433]
Mljekarstvo Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows []
留言咨询
Baidu
sogou