基质金属蛋白酶7(MMP7)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Matrix Metalloproteinase 7 (MMP7) ,etc. by FLIA (Flow Luminescence Immunoassay)
MPSL1; MAT; PUMP-1; Matrilysin Uterine; Matrilysin; Matrin; Pump-1 Protease; Uterine Metalloproteinase
(注:单次混测多因子不超过8个指标 )
- 编号LMA102Ra
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围4.88-5000pg/mL
- 灵敏度最小可检测剂量小于等于1.627 pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 8指标数 7指标数 6指标数 5指标数 4指标数 3指标数 2指标数1指标数
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特异性
本试剂盒用于检测基质金属蛋白酶7(MMP7)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的基质金属蛋白酶7(MMP7)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 92-99 | 95 |
EDTA plasma(n=5) | 82-93 | 86 |
heparin plasma(n=5) | 85-103 | 95 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
线性
在定值血清及血浆样本内加入适量的基质金属蛋白酶7(MMP7)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中基质金属蛋白酶7(MMP7)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 91-101% | 90-98% | 98-105% | 80-91% |
EDTA plasma(n=5) | 99-105% | 88-102% | 80-104% | 81-94% |
heparin plasma(n=5) | 90-97% | 97-104% | 93-101% | 93-101% |
稳定性
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠)标准品或样本100μL及磁珠10μL,
37°C酶标板振荡器孵育90分钟;
3. 磁吸甩干,加检测溶液A100μL,37°C酶标板振荡器孵育60分钟;
4. 磁吸洗板3次;
5. 加检测溶液B100μL,37°C振动孵育30分钟;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋涡震荡2分钟后读数。
实验原理
将基质金属蛋白酶7(MMP7)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠,其中的基质金属蛋白酶7(MMP7)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合,然后加入生物素化的基质金属蛋白酶7(MMP7)等多因子检测试剂盒(流式荧光发光法)抗体,将未结合的生物素化抗体洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的基质金属蛋白酶7(MMP7)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPA102Ra01 | 基质金属蛋白酶7(MMP7)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA102Ra01 | 基质金属蛋白酶7(MMP7)多克隆抗体 | WB; IHC; ICC; IP. |
MAA102Ra21 | 基质金属蛋白酶7(MMP7)单克隆抗体 | WB; IHC; ICC; IP. |
SEA102Ra | 基质金属蛋白酶7(MMP7)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA102Ra | 基质金属蛋白酶7(MMP7)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA102Ra | 基质金属蛋白酶7(MMP7)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
杂志 | 参考文献 |
British Journal of Pharmacology | A novel, orally active LPA1 receptor antagonist inhibits lung fibrosis in the mouse bleomycin model [PubMed: 20649573] |
Neuroimmunomodulation | Upregulated Expression of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinases in BALB/c Mouse Brain Challenged with Japanese Encephalitis Virus [Pubmed: source] |
PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655] |
Histol Histopathol | Estrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cells [PubMed: 26254457] |
VirusDisease | Circulating levels of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases during Japanese encephalitis virus infection [Pubmed:26925446] |
Am J Physiol Lung Cell Mol Physiol | Systematic phenotyping and correlation of biomarkers with lung function and histology in lung fibrosis [Pubmed:26993522] |
Cellular Physiology and Biochemistry | The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cells [pubmed:29224019] |
s-space | Thyroid disrupting effects and associated mechanisms of TCPP in GH3 cell line and zebrafish (Danio rerio) larva and adult [10371/137708] |
Science Translational Medicine | Large-scale proteomics identifies MMP-7 as a sentinel of epithelial injury and of biliary atresia. [pubmed:29167395] |
Hepatology | Diagnostic Accuracy of Serum Matrix Metalloproteinase‐7 for Biliary Atresia [Pubmed: 30153340] |
Journal of Pediatrics | Quantification of Serum Matrix Metallopeptide 7 Levels May Assist in the Diagnosis and Predict the Outcome for Patients with Biliary Atresia [Pubmed: 30853207] |
Pediatrics | Serum MMP-7 in the Diagnosis of Biliary Atresia [Pubmed: 31604829] |
Biomed Pharmacother | Human umbilical cord blood mononuclear cells protect against renal tubulointerstitial fibrosis in cisplatin-treated rats [Pubmed: 31710895] |
LABORATORY INVESTIGATION | The synthetic toxin biliatresone causes biliary atresia in mice [Pubmed: 32681026] |
diagnostics | Interstitial Score and Concentrations of IL-4R¦Á, PAR-2, and MMP-7 in Bronchoalveolar Lavage Fluid Could Be Useful Markers for Distinguishing Idiopathic Interstitial?¡ [33924683] |
Cancer Cell Int | A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer [34215253] |