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粘蛋白2(MUC2)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Mucin 2 (MUC2) ,etc. by FLIA (Flow Luminescence Immunoassay)

MLP; SMUC; Oligomeric Mucus/Gel-Forming; Mucin 2,Intestinal/Tracheal

（注：单次混测多因子不超过8个指标 ）

  • 粘蛋白2(MUC2)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 粘蛋白2(MUC2)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测粘蛋白2(MUC2)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
    37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将粘蛋白2(MUC2)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的粘蛋白2(MUC2)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的粘蛋白2(MUC2)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的粘蛋白2(MUC2)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPA705Mu02 粘蛋白2(MUC2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA705Mu03 粘蛋白2(MUC2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA705Mu01 粘蛋白2(MUC2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA705Mu02 粘蛋白2(MUC2)多克隆抗体 WB; IHC; ICC; IP.
PAA705Mu03 粘蛋白2(MUC2)多克隆抗体 WB; IHC; ICC; IP.
PAA705Mu01 粘蛋白2(MUC2)多克隆抗体 WB,IHC
LAA705Mu81 粘蛋白2(MUC2)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA705Mu71 粘蛋白2(MUC2)多克隆抗体(生物素标记) WB; IHC; ICC.
SEA705Mu 粘蛋白2(MUC2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA705Mu 粘蛋白2(MUC2)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA705Mu01 粘蛋白2(MUC2)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Annals of surgical oncology Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas. [PubMed: 20717734]
World J Gastroenterol. Sodium alginate ameliorates indomethacin-induced gastrointestinal mucosal injury via inhibiting translocation in rats. [Pubmed:24627600]
Front Microbiol Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis [PubMed: 26217323]
nutrition research and practice Butyrate modulates bacterial adherence on LS174T human colorectal cells by stimulating mucin secretion and MAPK signaling pathway [PubMed: 26244071]
Respirology Secreted mucins and airway bacterial colonization in non-CF bronchiectasis [PubMed: 26172851]
Ann Am Thorac Soc Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization [doi:10.1513]
Cellular Microbiology An adherent mucus layer attenuates the genotoxic effect of colibactin. [pubmed:29156489]
Bio-protocol Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA [10.21769:BioProtoc.2879]
Journal of Investigative Medicine The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease [Pubmed: 30573494]
Biomolecules Clinical Significance of Serum Membrane-Bound Mucin-2 Levels in Breast Cancer [Pubmed: 30682816]
Cell Stem Cell PRDM16 Maintains Homeostasis of the Intestinal Epithelium by Controlling Region-Specific Metabolism [Pubmed: 31564549]
DIGESTIVE DISEASES AND SCIENCES Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic Rats [Pubmed: 32144600]
Scientific Reports Mucus layer modeling of human colonoids during infection with enteroaggragative E. coli [Pubmed: 32601325]
Biorxiv Defective intestinal repair after short-term high fat diet due to loss of efferocytosis []
J Cell Mol Med Inhibition of cyclooxygenase©\2 enhanced intestinal epithelial homeostasis via suppressing ¦Â©\catenin signalling pathway in experimental liver fibrosis [34145945]
Cell Tissue Res Reduced acetylcholine and elevated muscarinic receptor 2 in duodenal mucosa contribute to the impairment of mucus secretion in 6-hydroxydopamine-induced … [34370080]
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