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中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Neutrophil Elastase (NE) ,etc. by FLIA (Flow Luminescence Immunoassay)

ELANE; HLE; HNE; ELA2; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Leukocyte Elastase; PMN Elastase

(注:单次混测多因子不超过8个指标 )

  • 中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 91-101 95
EDTA plasma(n=5) 79-101 90
heparin plasma(n=5) 97-104 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 86-97% 79-94% 78-94% 95-104%
EDTA plasma(n=5) 80-101% 91-103% 96-104% 90-99%
heparin plasma(n=5) 87-102% 88-99% 86-95% 84-99%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠)标准品或样本100μL及磁珠10μL,
    37°C酶标板振荡器孵育90分钟;
3. 磁吸甩干,加检测溶液A100μL,37°C酶标板振荡器孵育60分钟;
4. 磁吸洗板3次;
5. 加检测溶液B100μL,37°C振动孵育30分钟;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋涡震荡2分钟后读数。

实验原理

将中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠,其中的中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合,然后加入生物素化的中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法)抗体,将未结合的生物素化抗体洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
APA181Mu61 中性粒细胞弹性蛋白酶(NE)活性蛋白 Cell culture; Activity Assays.
RPA181Mu01 中性粒细胞弹性蛋白酶(NE)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA181Mu02 中性粒细胞弹性蛋白酶(NE)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA181Mu61 中性粒细胞弹性蛋白酶(NE)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA181Mu01 中性粒细胞弹性蛋白酶(NE)多克隆抗体 WB; IHC; ICC; IP.
PAA181Mu02 中性粒细胞弹性蛋白酶(NE)多克隆抗体 WB; IHC; ICC; IP.
SEA181Mu 中性粒细胞弹性蛋白酶(NE)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA181Mu 中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

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