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诱导型一氧化氮合酶(NOS2)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Nitric Oxide Synthase 2, Inducible (NOS2) ,etc. by FLIA (Flow Luminescence Immunoassay)

NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2

（注：单次混测多因子不超过8个指标 ）

  • 诱导型一氧化氮合酶(NOS2)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 诱导型一氧化氮合酶(NOS2)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测诱导型一氧化氮合酶(NOS2)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的诱导型一氧化氮合酶(NOS2)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 79-92 86
EDTA plasma(n=5) 82-92 87
heparin plasma(n=5) 88-102 96

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的诱导型一氧化氮合酶(NOS2)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中诱导型一氧化氮合酶(NOS2)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 95-103% 83-96% 79-97% 79-101%
EDTA plasma(n=5) 94-103% 91-103% 91-98% 99-105%
heparin plasma(n=5) 88-96% 98-105% 85-95% 92-101%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
    37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将诱导型一氧化氮合酶(NOS2)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的诱导型一氧化氮合酶(NOS2)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的诱导型一氧化氮合酶(NOS2)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的诱导型一氧化氮合酶(NOS2)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种：Oryctolagus cuniculus (Rabbit，兔) 应用(仅供研究使用，不用于临床诊断！)
RPA837Rb01 诱导型一氧化氮合酶(NOS2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA837Rb51 诱导型一氧化氮合酶(NOS2)多克隆抗体 WB; IHC; ICC; IP.
MAA837Rb21 诱导型一氧化氮合酶(NOS2)单克隆抗体 WB; IHC; ICC; IP.
SEA837Rb 诱导型一氧化氮合酶(NOS2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA837Rb 诱导型一氧化氮合酶(NOS2)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease High-molecular weight hyaluronan reduced renal PKC activation in genetically diabetic mice [PubMed: 20713153]
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease Hyaluronan reduces inflammation in experimental arthritis by modulating TLR-2 and TLR-4 cartilage expression [ScienceDirect: S0925443911001323]
FEBS Journal Adenosine A2a Receptor Activation and Hyaluronan Fragment Inhibition Reduce Inflammation in Mouse Articular Chondrocytes Stimulated with Il-1beta [Wiley: source]
Gene Hyaluronan in part mediates IL-1beta-induced inflammation in mouse chondrocytes by up-regulating CD44 receptors [ScienceDirect: S0378111911007347]
PLoS One A Novel Mechanism of Formaldehyde Neurotoxicity: Inhibition of Hydrogen Sulfide Generation by Promoting Overproduction of Nitric Oxide [PubMed: 23359814]
PLoS ONE Regulation of Microglia Activity by Glaucocalyxin-A: Attenuation of Lipopolysaccharide-Stimulated Neuroinflammation through NF-κB and p38 MAPK Signaling Pathways [PlosOne: Source]
Journal of Cellular Biochemistry The inhibition of hyaluronan degradation reduced pro-inflammatory cytokines in mouse synovial fibroblasts subjected to collagen-induced arthritis. [PubMed: 22234777]
International Immunopharmacology Amentoflavone inhibits iNOS, COX-2 expression and modulates cytokine profile, NF-κB signal transduction pathways in rats with ulcerative colitis [Pubmed: 24126114]
Integrative Cancer Therapies Decalepis hamiltonii inhibits tumor progression and metastasis by regulating the inflammatory mediators and nuclear factor κB subunits [Pubmed: 24013642]
Food and Chemical Toxicology Inhibition of UVB-induced skin phototoxicity by a grape seed extract as modulator of nitrosative stress, ERK/NF-kB signaling pathway and apoptosis, in SKH-1 mice [Pubmed: 23567245]
Experimental Gerontology Age-related changes in ADMA–DDAH–NO pathway in rat liver subjected to partial ischemia followed by global reperfusion [ScienceDirect: S0531556513003331]
International Immunopharmacology Protective effect of marine mangrove Rhizophora apiculata on acetic acid induced experimental colitis by regulating anti-oxidant enzymes, inflammatory mediators and nuclear factor-kappa B subunits [ScienceDirect: S1567576913004153]
INTERNATIONAL JOURNAL OF MEDICINE AND MEDICAL RESEARCH L-ARGININE, BUT NOT L-NAME PROTECTS AGAINST LIVER INJURY INDUCED BY EXPERIMENTAL ISCHEMIA-REPERFUSION [Tdmu:Source]
Molecular Vision Effects of topically applied tocotrienol on cataractogenesis and lens redox status in galactosemic rats [Pubmed:24940038]
Journal of Environmental Pathology, Toxicology and Oncology Protective Effect of?Acacia ferruginea?against Ulcerative Colitis via Modulating Inflammatory Mediators, Cytokine Profile and NF-κB Signal Transduction Pathways [Pubmed:24941292]
Eur J Cancer Prev. Utility of Apc-mutant rats with a colitis-associated colon carcinogenesis model for chemoprevention studies [Pubmed:25222193]
Cell and Tissue Research Beta-arrestin 1 is involved in the catabolic response stimulated by hyaluronan degradation in mouse chondrocytes [Pubmed:25673209]
Ann N Y Acad Sci.? Modulating effect of Biophytum sensitivum extract on rats with acetic acid-induced ulcerative colitis [Pubmed:5243883]
Molecular Medicine Reports Resveratrol ameliorates oxidative stress and inhibits aquaporin 4 expression following rat cerebral ischemia-reperfusion injury [PubMed: 26458999]
Chem Biol Interact Apocynin protects against ethanol-induced gastric ulcer in rats by attenuating the upregulation of NADPH oxidases 1 and 4 [PubMed: 26522475]
Journal of Environmental Pathology, Toxicology and Oncology Protective Effect of [PubMed: 26349607]
Journal of Immunotoxicology Acacia ferruginea inhibits inflammation by regulating inflammatory iNOS and COX-2 [PubMed: 25738525]
Cancer Medicine Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host disease [Pubmed:27109254]
Journal of Natural Medicines Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark of Lycium chinense (Cortex Lycii) against gastric ulcer in mice [Pubmed:26972100]
Cell Mol Immunol  Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica [Pubmed:27157496]
Медицина и здравоохранение Роль индуцибельной NO-синтазы в механизмах регуляции функциональной активности кальций-активируемых калиевых каналов коронарных сосудов у крыс, адаптированных к стрессу [article:n]
Graefe's Archive for Clinical and Experimental Ophthalmology Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome [pubmed:27538909]
Taylor & Francis Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation [09540105.2017.1306494]
Molecular Vision Mechanism of the anticataract effect of liposomal MgT in galactose-fed rats. [pubmed:27440992]
Journal of Natural Medicines Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark ofLycium chinense (Cortex Lycii) against gastric ulcer in mice. [pubmed:26972100]
Applied Biochemistry and Biotechnology Protective Effect of Zingiber officinale Against Dalton's Lymphoma Ascites Tumour byRegulating Inflammatory Mediator and Cytokines. [pubmed:27435276]
Cell Mol Neurobiol Posttraumatic Stress Disorder Disturbs Coronary Tone and Its Regulatory Mechanisms [pubmed:28676988]
Microbial Immunology Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiota [pubmed:28713364]
Biomedicine & Pharmacotherapy Protective effect of Averrhoa bilimbi L. fruit extract on ulcerative colitis in wistar rats via regulation of inflammatory mediators and cytokines [pubmed:28531922]
The Royal Society of Chemistry Anti-ulcerogenic effect of KFP-H008 against ethanol-induced gastric ulcer via p38 MAPK/NF-κB pathway [10.1039/C7RA08879E]
Acta Pharmacologica Sinica Cis-stilbene glucoside in Polygonum multiflorum induces immunological idiosyncratic hepatotoxicity in LPS-treated rats by suppressing PPAR-γ. [pubmed:28649126]
Cytokine Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects [pubmed:29102164]
Molecules Melatonin balance the autophagy and apoptosis by regulating UCP2 in the LPS-induced cardiomyopathy [Pubmed:29547569]
European Journal of Pharmacology  Naringenin ameliorates learning and memory impairment following systemic lipopolysaccharide challenge in the rat [Pubmed:29518393]
Regulatory Toxicology and Pharmacology Targeted inhibition of tumor survival, metastasis and angiogenesis by Acacia ferruginea mediated regulation of VEGF, inflammatory mediators, cytokine profile and … [Pubmed:29678767]
Pharmacological Reports The Association between Inflammatory Markers (iNOS, HO-1, IL-33, MIP-1β) and Depression with and without Posttraumatic Stress Disorder [10.1016:j.pharep.2018.06.001]
Clinical And Experimental Pharmacology And Physiology  Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway [Pubmed:29266336]
Stężenia śródbłonkowej i indukowalnej formy syntazy tlenku azotu w surowicy krwi kobiet ciężarnych palących tytoń–badania wstępne []
Journal of Allergy and Clinical Immunology Exosome swarms eliminate airway pathogens and provide passive epithelial immunoprotection through nitric oxide [Pubmed: 30442371]
Metabolic Brain Disease Trigonelline protects hippocampus against intracerebral Aβ (1–40) as a model of Alzheimer's disease in the rat: insights into underlying mechanisms [Pubmed: 30421246]
PLoS One Intraphagolysosomal conditions predispose to Staphylococcus epidermidis small colony variants persistence in macrophages [Pubmed: 30412620]
The Eurasian Journal of Medicine Tadalafil Preserves Penile Nitric Oxide Synthase from Detrimental Effect of Paroxetine in Rats [Doi: 10.5152/eurasianjmed.2018.18160]
PLoS One Macrophage expression of E3 ubiquitin ligase Grail protects mice from lipopolysaccharide-induced hyperinflammation and organ injury [Pubmed: 30571701]
International Journal of Environmental Research and Public Health Influence of Active Exposure to Tobacco Smoke on Nitric Oxide Status of Pregnant Women [Pubmed: 30513890]
Journal of Functional Foods Complex interaction of dietary fat and Alaskan bog blueberry supplementation influences manganese mediated neurotoxicity and behavioral impairments []
Particle and Fibre Toxicology Silica nanoparticles trigger the vascular endothelial dysfunction and prethrombotic state via miR-451 directly regulating the IL6R signaling pathway [Pubmed: 30975181]
ВЛИЯНИЕ ИНДУЦИБЕЛЬНОЙ NO-СИНТАЗЫ НА ТОНУС КОРОНАРНЫХ СОСУДОВ, СОКРАТИТЕЛЬНУЮ ФУНКЦИЮ МИОКАРДА И МЕХАНИЗМЫ … []
Journal of Cosmetic Dermatology Whitening effects of cosmetic formulation in the vascular component of skin pigmentation [Pubmed: 31074159]
regional hemodynamics and microcirculation Benzimidazole Derivative, 2-ETH Prevents Poststressor Disorders of Coronary Vascular Tone and Myocardial Contractility []
Life Sci Cilostazol mitigates mesenteric ischemia/reperfusion-induced lung lesion: Contribution of PPAR-¦Ã, NF-¦ÊB, and STAT3 crosstalk [33310046]
PLoS One Correlation analysis of the proportion of monocytic myeloid-derived suppressor cells in colorectal cancer patients [33370317]
Biotechnol Rep Targeting NF-¦ÊB mediated cell signaling pathway and inflammatory mediators by 1, 2-diazole in A549 cells in vitro [33598414]
Basic and Clinical Neuroscience Paeonol Protects Against Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson's Disease [33995926]
Fermented Foods and Microbes Related to Health Cordyceps cicadae NTTU 868 Mycelium with The Addition of Bioavailable Forms of Magnesium from Deep Ocean Water Prevents the A¦Â40 and Streptozotocin?¡­ []
Ya?lanma ve Hipertansiyonda Yeni bir Endoltelyal Disfonksiyon Belirteci: ??z¨¹nebilir Lektin Benzeri Okside D¨¹?¨¹k Dansiteli Lipoprotein-1 []
Fermentation Cordyceps cicadae NTTU 868 Mycelium with The Addition of Bioavailable Forms of Magnesium from Deep Ocean Water Prevents the A¦Â40 and Streptozotocin-Induced Memory Deficit via Suppressing Alzheimer¡¯s Disease Risk Factors and Increasing Magnesium Uptake of Brain []
Drug Chem Toxicol Study of cardioprotective activity of the methanolic extract of the aerial parts of Bauhinia madagascariensis compared to Bauhinia purpurea against adrenaline … [34167392]
Frontiers in Cardiovascular Medicine The Effects of Intermittent Hypoxic–Hyperoxic Exposures on Lipid Profile and Inflammation in Patients With Metabolic Syndrome [34513946]
Nutrients Erinacine A-Enriched Hericium erinaceus Mycelium Delays Progression of Age-Related Cognitive Decline in Senescence Accelerated Mouse Prone 8 (SAMP8) Mice [34684662]
biomolecules Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men [34356664]
Hum Exp Toxicol Carvacrol hinders the progression of hepatic fibrosis via targeting autotaxin and thioredoxin in thioacetamide-induced liver fibrosis in rat [34155936]
J Ethnopharmacol Post-stroke treatment of storax improves long-term outcomes of stroke in rats [34343648]
Phytomedicine A multidimensional strategy for uncovering comprehensive quality markers of Schisandra chinensis (Turcz.) Baill based on pharmacodynamics and chemical … []
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