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YY肽(PYY)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Peptide YY (PYY) ,etc. by FLIA (Flow Luminescence Immunoassay)

PYY1; Peptide tyrosine tyrosine

(注:单次混测多因子不超过8个指标 )

  • YY肽(PYY)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • YY肽(PYY)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测YY肽(PYY)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的YY肽(PYY)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 98-105 102
EDTA plasma(n=5) 78-101 90
heparin plasma(n=5) 92-101 96
sodium citrate plasma(n=5) 96-104 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的YY肽(PYY)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中YY肽(PYY)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 80-99% 79-88% 83-91% 93-105%
EDTA plasma(n=5) 81-105% 78-94% 89-96% 99-105%
heparin plasma(n=5) 91-99% 83-94% 82-89% 85-96%
sodium citrate plasma(n=5) 92-105% 89-101% 95-102% 88-98%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠、检测溶液A)标准品或样本50μL及磁珠10μL,加检测溶液A50μL,
    37°C酶标板振荡器孵育60分钟;
3. 磁吸洗板3次;
4. 加检测溶液B100μL,37°C振动孵育30分钟;
5. 磁吸洗板3次;
6. 加鞘液100μL,旋涡震荡2分钟后读数。

实验原理

将YY肽(PYY)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠、标记VEGFA,标记的YY肽(PYY)等多因子检测试剂盒(流式荧光发光法)和未标记的YY肽(PYY)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合,然后将未结合物洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的YY肽(PYY)等多因子检测试剂盒(流式荧光发光法)呈负相关。

赠品

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPB067Mu02 YY肽(PYY)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB067Mu01 YY肽(PYY)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
SPB067Mu01 YY肽(PYY)合成多肽 Immunogen; Blocking Peptide.
CPB067Mu21 YY肽(PYY)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAB067Mu02 YY肽(PYY)多克隆抗体 WB; IHC; ICC; IP.
PAB067Mu01 YY肽(PYY)多克隆抗体 WB; IHC; ICC; IP.
CEB067Mu YY肽(PYY)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEB067Mu YY肽(PYY)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB067Mu YY肽(PYY)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB067Mu11 YY肽(PYY)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
International Journal of Sport Studies The Effects of Concurrent Resistance and Endurance Exercise on Hunger Feelings and PYY in Obese Men [Com:Source]
Annals of Applied Sport Science Effect of an Acute Incremental Exercise on Plasma Peptide YY, Neuropeptide Y and IGF-1 Concentrations in Young Athletes [Aassjournal:Source]
Biomedical Reports Calprotectin in serum and zonulin in serum and feces are elevated after introduction of a diet with lower carbohydrate content and higher fiber, fat and protein contents. [pubmed:28413639]
Acta Diabetologica Effect of lifestyle improvement program on the biomarkersof adiposity, inflammation and gut hormones in overweight/obese Asian Indians with prediabetes [pubmed:28620678]
Biomedical Reports Calprotectin in serum and zonulin in serum and feces are elevated after introduction of a diet with lower carbohydrate content and higher fiber, fat and protein contents [10.3892/br.2017.865]
Journal of Diabetes & Metabolism High Fiber Fat and Protein Contents Lead to Increased Satiety Reduced Sweet Cravings and Decreased Gastrointestinal Symptoms Independently of Anthropometric Hormonal and Metabolic Factors [10.4172/2155-6156.1000733 ]
Frontiers in Physiology Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 [Pubmed:29593555]
Food and Nutrition Research Alignments of endocrine, anthropometric, and metabolic parameters in type 2 diabetes after intervention with an Okinawa-based Nordic diet [Pubmed:29599686]
The Journal of Nutrition Grape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned Pigs [Pubmed: 31175811]
The Journal of Nutritional Biochemistry Bile acid receptor TGR5 is critically involved in preference for dietary lipids and obesity [Pubmed: 31812910]
Arch Toxicol Serotonin involvement in okadaic acid-induced diarrhoea in vivo [34148100]
foods Appetite and Satiety Effects of the Acute and Regular Consumption of Green Coffee Phenols and Green Coffee Phenol/Oat β-Glucan Nutraceuticals in Subjects with … [34828792]
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