YY肽(PYY)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Peptide YY (PYY) ,etc. by FLIA (Flow Luminescence Immunoassay)
PYY1; Peptide tyrosine tyrosine
(注:单次混测多因子不超过8个指标 )
- 编号LMB067Mu
- 物种Mus musculus (Mouse,小鼠) 相同的名称,不同的物种。
- 实验方法竞争抑制
- 反应时长1.5h
- 检测范围0.98-1000pg/mL
- 灵敏度最小可检测剂量小于等于0.327 pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 8指标数 7指标数 6指标数 5指标数 4指标数 3指标数 2指标数1指标数
- 价格 ¥ 2562 ¥ 2660 ¥ 2808 ¥ 3005 ¥ 3202 ¥ 3497 ¥ 3941 计算器 ¥ 4926 添加到价格计算器
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
本试剂盒用于检测YY肽(PYY)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的YY肽(PYY)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 98-105 | 102 |
EDTA plasma(n=5) | 78-101 | 90 |
heparin plasma(n=5) | 92-101 | 96 |
sodium citrate plasma(n=5) | 96-104 | 101 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
线性
在定值血清及血浆样本内加入适量的YY肽(PYY)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中YY肽(PYY)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 80-99% | 79-88% | 83-91% | 93-105% |
EDTA plasma(n=5) | 81-105% | 78-94% | 89-96% | 99-105% |
heparin plasma(n=5) | 91-99% | 83-94% | 82-89% | 85-96% |
sodium citrate plasma(n=5) | 92-105% | 89-101% | 95-102% | 88-98% |
稳定性
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠、检测溶液A)标准品或样本50μL及磁珠10μL,加检测溶液A50μL,
37°C酶标板振荡器孵育60分钟;
3. 磁吸洗板3次;
4. 加检测溶液B100μL,37°C振动孵育30分钟;
5. 磁吸洗板3次;
6. 加鞘液100μL,旋涡震荡2分钟后读数。
实验原理
将YY肽(PYY)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠、标记VEGFA,标记的YY肽(PYY)等多因子检测试剂盒(流式荧光发光法)和未标记的YY肽(PYY)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合,然后将未结合物洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的YY肽(PYY)等多因子检测试剂盒(流式荧光发光法)呈负相关。
赠品
增值服务
相关产品
编号 | 适用物种:Mus musculus (Mouse,小鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPB067Mu02 | YY肽(PYY)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB067Mu01 | YY肽(PYY)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
SPB067Mu01 | YY肽(PYY)合成多肽 | Immunogen; Blocking Peptide. |
CPB067Mu21 | YY肽(PYY)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
PAB067Mu02 | YY肽(PYY)多克隆抗体 | WB; IHC; ICC; IP. |
PAB067Mu01 | YY肽(PYY)多克隆抗体 | WB; IHC; ICC; IP. |
CEB067Mu | YY肽(PYY)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEB067Mu | YY肽(PYY)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB067Mu | YY肽(PYY)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSB067Mu11 | YY肽(PYY)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
International Journal of Sport Studies | The Effects of Concurrent Resistance and Endurance Exercise on Hunger Feelings and PYY in Obese Men [Com:Source] |
Annals of Applied Sport Science | Effect of an Acute Incremental Exercise on Plasma Peptide YY, Neuropeptide Y and IGF-1 Concentrations in Young Athletes [Aassjournal:Source] |
Biomedical Reports | Calprotectin in serum and zonulin in serum and feces are elevated after introduction of a diet with lower carbohydrate content and higher fiber, fat and protein contents. [pubmed:28413639] |
Acta Diabetologica | Effect of lifestyle improvement program on the biomarkersof adiposity, inflammation and gut hormones in overweight/obese Asian Indians with prediabetes [pubmed:28620678] |
Biomedical Reports | Calprotectin in serum and zonulin in serum and feces are elevated after introduction of a diet with lower carbohydrate content and higher fiber, fat and protein contents [10.3892/br.2017.865] |
Journal of Diabetes & Metabolism | High Fiber Fat and Protein Contents Lead to Increased Satiety Reduced Sweet Cravings and Decreased Gastrointestinal Symptoms Independently of Anthropometric Hormonal and Metabolic Factors [10.4172/2155-6156.1000733 ] |
Frontiers in Physiology | Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 [Pubmed:29593555] |
Food and Nutrition Research | Alignments of endocrine, anthropometric, and metabolic parameters in type 2 diabetes after intervention with an Okinawa-based Nordic diet [Pubmed:29599686] |
The Journal of Nutrition | Grape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned Pigs [Pubmed: 31175811] |
The Journal of Nutritional Biochemistry | Bile acid receptor TGR5 is critically involved in preference for dietary lipids and obesity [Pubmed: 31812910] |
Arch Toxicol | Serotonin involvement in okadaic acid-induced diarrhoea in vivo [34148100] |
foods | Appetite and Satiety Effects of the Acute and Regular Consumption of Green Coffee Phenols and Green Coffee Phenol/Oat β-Glucan Nutraceuticals in Subjects with … [34828792] |