核因子κB受体激活因子配体(RANkL)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) ,etc. by FLIA (Flow Luminescence Immunoassay)
CD254; TNFSF11; ODF; OPGL; TRANCE; HRANKL2; SOdf; Tumor Necrosis Factor(ligand)superfamily Member 11; TNF-related activation-induced cytokine
（注：单次混测多因子不超过8个指标 ）
- 编号LMA855Mu
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围0.98-1000pg/mL
- 灵敏度最小可检测剂量小于等于0.327 pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测核因子κB受体激活因子配体(RANkL)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的核因子κB受体激活因子配体(RANkL)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 81-102 | 86 |
| EDTA plasma(n=5) | 78-92 | 85 |
| heparin plasma(n=5) | 79-104 | 85 |
| sodium citrate plasma(n=5) | 88-99 | 92 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的核因子κB受体激活因子配体(RANkL)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中核因子κB受体激活因子配体(RANkL)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 78-88% | 89-103% | 79-90% |
| EDTA plasma(n=5) | 83-102% | 92-105% | 85-96% | 95-103% |
| heparin plasma(n=5) | 81-94% | 85-97% | 80-104% | 80-88% |
| sodium citrate plasma(n=5) | 80-89% | 95-104% | 96-103% | 84-101% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将核因子κB受体激活因子配体(RANkL)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的核因子κB受体激活因子配体(RANkL)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的核因子κB受体激活因子配体(RANkL)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的核因子κB受体激活因子配体(RANkL)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
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相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| EPA855Mu62 | 核因子κB受体激活因子配体(RANkL)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA855Mu61 | 核因子κB受体激活因子配体(RANkL)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA855Mu01 | 核因子κB受体激活因子配体(RANkL)活性蛋白 | Cell culture; Activity Assays. |
| RPA855Mu01 | 核因子κB受体激活因子配体(RANkL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA855Mu61 | 核因子κB受体激活因子配体(RANkL)活性蛋白 | Cell culture; Activity Assays. |
| RPA855Mu02 | 核因子κB受体激活因子配体(RANkL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA855Mu01 | 核因子κB受体激活因子配体(RANkL)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA855Mu02 | 核因子κB受体激活因子配体(RANkL)多克隆抗体 | WB; IHC; ICC; IP. |
| SEA855Mu | 核因子κB受体激活因子配体(RANkL)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA855Mu | 核因子κB受体激活因子配体(RANkL)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Archives of Oral Biology | Compressive stress induced the up-regulation of M-CSF, RANKL, TNF-α expression and the down-regulation of OPG expression in PDL cells via the integrin-FAK pathway [ScienceDirect: S0003996912003998] |
| PLOS One | Adipokines in Psoriatic Arthritis Patients: The Correlations with Osteoclast Precursors and Bone Erosions [PubMed: PMC3483160] |
| Arthritis Research&Therapy | Regulatory effect of calcineurin inhibitor, tacrolimus, on IL-6/sIL-6R-mediated RANKL expression through JAK2-STAT3-SOCS3 signaling pathway in fibroblast-like synoviocytes [PubMed: PMC3672788] |
| International Journal of Oral and Maxillofacial Surgery | The effects of chronic zoledronate usage on the jaw and long bones evaluated using RANKL and osteoprotegerin levels in an animal model [Pubmed: 23522850] |
| AGE | Estrogen deficiency does not decrease the in vitro osteogenic potential of rat adipose-derived mesenchymal stem cells [Pubmed: 24687841] |
| Food Science & Nutrition | Effects and mechanisms of 8-prenylnaringenin on osteoblast MC3T3-E1 and osteoclast-like cells RAW264.7 [Onlinelibrary: fsn3.109] |
| Food Sci Nutr. | Effects and mechanisms of 8‐prenylnaringenin on osteoblast MC3T3‐E1 and osteoclast‐like cells RAW264. 7 [Pubmed:Pmc4221832] |
| Journal of Investigative Surgery | Effect of Nicotine on RANKL and OPG and Bone Mineral Density [Pubmed:24830656] |
| Int J Mol Med. | Effects of icariin on the regulation of the OPG?RANKL?RANK system are mediated through the MAPK pathways in IL?1β?stimulated human SW1353 chondrosarcoma … [Pubmed:25270538] |
| Clinical and Experimental Pharmacology and Physiology | Carvedilol promotes neurological function, reduces bone loss and attenuates cell damage after acute spinal cord injury in rats [Pubmed:25424914] |
| Int J Clin Exp Pathol | A novel model of bisphosphonate-related osteonecrosis of the jaw in rats [PubMed: 26191212] |
| oncotarget | MicroRNA-106b inhibits osteoclastogenesis and osteolysis by targeting RANKL in giant cell tumor of bone [PubMed: 26053181] |
| European Journal of Clinical Investigation | Serum osteopontin levels are upregulated and predict disability after an ischaemic stroke [PubMed: 25845543] |
| Scienze Biomediche | Effetti del laser a bassa dose su cellule osteoblastiche [INCERTIPARENTI_SERENA_TESI.pdf] |
| Nutrition. | Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats. [pubmed:27908549] |
| PLoS One. | Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats. [pubmed:28158228] |
| Epilepsy Research | The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats [pubmed:27838501] |
| Int Endod J. | Odontoclastogenesis of mouse papilla-derived MDPC-23 cells induced by lipopolysaccharide. [pubmed:28333374] |
| Sci Rep. | Sprint Interval Training Induces A Sexual Dimorphism but does not Improve Peak Bone Mass in Young and Healthy Mice. [pubmed:28303909] |
| Sci Rep. | Skeletal Site-specific Effects of Zoledronate on in vivo Bone Remodeling and in vitro BMSCs Osteogenic Activity. [pubmed:28139685] |
| Biomedicine & Pharmacotherapy | Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats [S0753332216322752] |
| BMJ Journals | SAT0003 The Role of 12/15-Lipoxygenase in The Pathogenesis of Osteoporosis in Mice [content:75] |
| Japanese Society for Bone and Mineral Research | Allogeneic yet major histocompatibility complex‑matched bone marrow transplantation in mice results in an impairment of osteoblasts and a significantly reduced trabecular bone [article:10.1007] |
| ONCOLOGY LETTERS | Assessment of bone metabolism and biomechanical properties of the femur, following treatment with anastrozole and letrozole in an experimental model of menopause [ol.2017.6596] |
| Journal of Clinical Periodontology | Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats [Pubmed:29574908] |
| Journal of Functional Foods | Effect of long-term administration of mangiferin from on bone metabolism in ovariectomized rats [10.1016:j.jff.2018.04.048] |
| International Immunopharmacology | Switched memory B cells promote alveolar bone damage during periodontitis: An adoptive transfer experiment [Pubmed:30015235] |
| European Journal of Pharmacology | The effect of levetiracetam on rat bone mineral density, bone structure and biochemical markers of bone metabolism [Pubmed:29428468] |
| Journal of Bone and Mineral Metabolism | Allogeneic yet major histocompatibility complex-matched bone marrow transplantation in mice results in an impairment of osteoblasts and a significantly reduced … [Pubmed:28766138] |
| Experimental and Therapeutic Medicine | Liraglutide exerts a bone‑protective effect in ovariectomized rats with streptozotocin‑induced diabetes by inhibiting osteoclastogenesis [Pubmed:29805533] |
| Bone | The sole and combined effect of simvastatin and platelet rich fibrin as a filling material in induced bone defect in tibia of albino rats [Pubmed: 30208342] |
| Journal of Periodontal Research | Improved RANKL expression and osteoclastogenesis induction of CD27+ CD38− memory B cells: A link between B cells and alveolar bone damage in periodontitis [Pubmed: 30346027] |
| Bone Research | NUMB maintains bone mass by promoting degradation of PTEN and GLI1 via ubiquitination in osteoblasts [Pubmed: 30455992] |
| Journal of International Dental and Medical Research | Alendronate Effect in RANKL Concentration at Ovariectomized Sprague Dawley Rat. [] |
| Bone | Development and characterization of supramolecular calcitonin assembly and assessment of its interactions with the bone remodelling process [Pubmed: 30797058] |
| Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology | Evaluation of therapeutic effects of teriparatide in a rat model of zoledronic acid-induced bisphosphonate-related osteonecrosis [] |
| Rheumatology International | Comparison of RANKL expression, inflammatory markers, and cardiovascular risk in patients with acute coronary syndrome with and without rheumatoid arthritis [Pubmed: 31297563] |
| World Journal of Pediatrics | Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis [Pubmed: 31641939] |
| Bone | Mice lacking plastin-3 display a specific defect of cortical bone acquisition [Pubmed: 31678489] |
| BIOSCIENCE REPORTS | IL-38 restrains inflammatory response of collagen-induced arthritis in rats via SIRT1/HIF-1α signaling pathway [Pubmed: 32347300] |
| Journal of Ethnopharmacology | Anti-osteoporosis effect of Semen Cuscutae in ovariectomized mice through inhibition of bone resorption by osteoclasts [34801609] |
| Br J Haematol | Inducible T‐cell co‐stimulator (ICOS) and ICOS ligand are novel players in the multiple‐myeloma microenvironment [Pubmed:34954822] |
| Biomed Pharmacother | Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axis [Pubmed:35453007] |
| Phytomedicine | Transcriptomic profiling revealed the role of apigenin-4′-O-α-L-rhamnoside in inhibiting the activation of rheumatoid arthritis fibroblast-like synoviocytes via MAPK … [Pubmed:35660352] |
| Med Science | Identification of marker proteins in preterm and term birth: Relationship of rankl and HSP70 in the cord blood [] |
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