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S100钙结合蛋白A9(S100A9)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for S100 Calcium Binding Protein A9 (S100A9) ,etc. by FLIA (Flow Luminescence Immunoassay)

MRP14; MIF; 60B8AG; CFAG; NIF; CAGB; CGLB; L1AG; P14; Calgranulin B; Migration inhibitory factor-related protein 14; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain

（注：单次混测多因子不超过8个指标 ）

  • S100钙结合蛋白A9(S100A9)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • S100钙结合蛋白A9(S100A9)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测S100钙结合蛋白A9(S100A9)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的S100钙结合蛋白A9(S100A9)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 80-90 80
EDTA plasma(n=5) 82-91 86
heparin plasma(n=5) 79-90 83
sodium citrate plasma(n=5) 93-105 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的S100钙结合蛋白A9(S100A9)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中S100钙结合蛋白A9(S100A9)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 84-103% 86-94% 78-105% 86-97%
EDTA plasma(n=5) 87-94% 90-104% 92-101% 79-90%
heparin plasma(n=5) 80-101% 78-90% 80-103% 87-98%
sodium citrate plasma(n=5) 78-102% 84-93% 97-104% 79-91%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
    37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将S100钙结合蛋白A9(S100A9)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的S100钙结合蛋白A9(S100A9)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的S100钙结合蛋白A9(S100A9)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的S100钙结合蛋白A9(S100A9)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPB793Mu02 S100钙结合蛋白A9(S100A9)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB793Mu01 S100钙结合蛋白A9(S100A9)活性蛋白 Cell culture; Activity Assays.
RPB793Mu01 S100钙结合蛋白A9(S100A9)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB793Mu01 S100钙结合蛋白A9(S100A9)多克隆抗体 WB; IHC; ICC; IP.
PAB793Mu02 S100钙结合蛋白A9(S100A9)多克隆抗体 WB; IHC; ICC; IP.
LAB793Mu81 S100钙结合蛋白A9(S100A9)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAB793Mu71 S100钙结合蛋白A9(S100A9)多克隆抗体(生物素标记) WB; IHC; ICC.
SEB793Mu S100钙结合蛋白A9(S100A9)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB793Mu S100钙结合蛋白A9(S100A9)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB793Mu01 S100钙结合蛋白A9(S100A9)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
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Journal of Alzheimer's Disease Proteomics Analysis of Blood Serums from Alzheimer's Disease Patients Using iTRAQ LabelingTechnology. [pubmed:27911324]
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Gastroenterology Tu1938-Micronutrients Assessment in Patients with Cirrhosis [10.1016:S0016-5085(18)33544-3]
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Estudio de células inmunosupresoras y biomarcadores circulantes en carcinoma renal []
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中国医药导报 降钙素原, 超敏C 反应蛋白, 红细胞沉降率及S100A9 对肺结核合并肺部细菌感染的影响 []
scandinavian journal of clinical & laboratory investigation Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study [Pubmed: 31856598]
PLoS One Adverse reproductive effects of S100A9 on bovine sperm and early embryonic development in vitro [Pubmed: 31945120]
Sci Rep Overexpression of S100A9 in tumor stroma contribute to immune evasion of NK/T cell lymphoma and predict poor response rate [34045609]
university of copenhagen Adjunctive S100A8/A9 Immunomodulation Hinders Cipro fl oxacin Resistance in []
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