分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Secretory Immunoglobulin A (sIgA) ,etc. by FLIA (Flow Luminescence Immunoassay)
S-IgA
（注：单次混测多因子不超过8个指标 ）
- 编号LMA641Ga
- 物种Chicken (Gallus，鸡) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长1.5h
- 检测范围48.83-50000pg/mL
- 灵敏度最小可检测剂量小于等于16.277 pg/mL.
- 样本类型Serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 81-104 | 95 |
| EDTA plasma(n=5) | 89-97 | 93 |
| heparin plasma(n=5) | 84-105 | 99 |
| sodium citrate plasma(n=5) | 94-102 | 97 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 80-101% | 84-95% | 97-104% |
| EDTA plasma(n=5) | 78-97% | 95-105% | 80-88% | 97-105% |
| heparin plasma(n=5) | 90-105% | 81-88% | 89-103% | 90-105% |
| sodium citrate plasma(n=5) | 80-90% | 87-101% | 96-105% | 81-98% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法)和未标记的分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法)呈负相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Chicken (Gallus，鸡) | 应用(仅供研究使用，不用于临床诊断！) |
| CEA641Ga | 分泌型免疫球蛋白A(sIgA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA641Ga | 分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| British Journal of Nutrition | Evaluation of the immune benefits of two probiotic strains Bifidobacterium animalis ssp. lactis, BB-12® and Lactobacillus paracasei ssp. paracasei, L. casei 431® in an influenza vaccination model: a randomised, double-blind, placebo-controlled study. [Solgar: source] |
| Jornal teknologi dan industri pangan | RESPON IMUN MUKOSA DAN SELULER PADA TIKUS YANG DIBERI BUBUK SUSU KAMBING DENGAN INFEKSI Salmonella Typhimurium [Mucosal and Cellular Immune Response of Rat Given Goat Milk Powder and Infected with Salmonella Typhimurium] [Ipb: Source] |
| Poultry Science | Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88 [Pubmed: 24570422 ] |
| Journal of Food Research | Potency of Lactobacillus plantarum Dad-13 and Sweet Potato (Ipomoea batatas) Fiber as Immunomodulator in Rats Infected With Salmonella Typhimurium [Ccsenet:Source] |
| PLoS One. | Repeated Oronasal Exposure to Lipopolysaccharide Induced Mucosal IgA Responses in Periparturient Dairy Cows [Pubmed:25061754] |
| Bioscience, Biotechnology, and Biochemistry | Dietary Apostichopus japonicus enhances the respiratory and intestinal mucosal immunity in immunosuppressive mice [Pubmed:25186733] |
| Food & Function | Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic mice [Pubmed:25308407] |
| Front Microbiol | Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis [PubMed: 26217323] |
| J Hum Lact. | Role of Maternal Allergy on Immune Markers in Colostrum and Secretory Immunoglobulin A in Stools of Breastfed Infants [PubMed: 26243755] |
| Pediatrics | Oropharyngeal Colostrum Administration in Extremely Premature Infants: An RCT [PubMed: 25624376] |
| Biomed Res Int. | Salivary Secretory Immunoglobulin (SIgA) and Lysozyme in Malignant Tumor Patients [journals:8701423] |
| 动物营养学报 | 益生菌抵御大鼠急性腹泻的效果及其对肠道黏膜屏障的影响 [article:12422] |
| Sci Rep. | Long-term use of ceftriaxone sodium induced changes in gut microbiota and immune system. [pubmed:28220870] |
| Acta Vet Hung. | Imbalance of intestinal immune function in piglets infected by porcine circovirus type 2 during the fetal period. [pubmed:28244338] |
| Food & Function | Immunomodulatory effects of Hericium erinaceus derived polysaccharides are mediated by intestinal immunology [pubmed:28266682] |
| Pathogens and Disease | Saccharomyces boulardiiadministration can inhibit the formation of gastric lymphoid follicles induced by Helicobacter suis infection [pubmed:28115360] |
| poultry science | Effects of dietary threonine supplementation on intestinal barrier function and gut microbiota of laying hens [pubmed:28938780] |
| Journal of Functional Foods | Novel dietary polysaccharide SIP promotes intestinal secretory immunoglobulin A secretion in mice under chemotherapy [10.1016/j.jff.2017.08.012] |
| International Journal of Pharmacology | Evaluation of Susceptibility and Innate Immune Response to Candida albicans in Mice with Sub-health [ijp.2018.689.697] |
| Journal of Dental Research | Correlation of s-IgA and IL-6 Salivary with Caries Disease and Oral Hygiene Parameters in Children [Pubmed: 31892186] |
| Analytical Chemistry | Fluorescence polarization-based rapid detection system for salivary biomarkers using modified DNA aptamers containing base-appended bases [Pubmed: 31855403] |
| Eur J Pharmacol | Artesunate protects immunosuppression mice induced by glucocorticoids via enhancing pro-inflammatory cytokines release and bacterial clearance [Pubmed: 33045197] |
| RESEARCH IN VETERINARY SCIENCE | Effects of different doses lipopolysaccharides on the mucosal barrier in mouse intestine [Pubmed: 32947071] |
| JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | Yacón (Smallanthus sonchifolius) prevented inflammation, oxidative stress, and intestinal alterations in an animal model of colorectal carcinogenesis [Pubmed: 32567144] |
| Vaccines | Construction and Immunogenicity Comparison of Three Virus-Like Particles Carrying Different Combinations of Structural Proteins of Avian Coronavirus Infectious?¡ [33670249] |
| Nutrients | Ingestion of High ¦Â-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects [33799564] |
| J Orofac Orthop | Assessment of salivary stress and pain biomarkers and their relation to self-reported pain intensity during orthodontic tooth movement: a longitudinal and prospective … [34170330] |
| Nutrients | Microarray Analysis of Paramylon, Isolated from Euglena Gracilis EOD-1, and Its Effects on Lipid Metabolism in the Ileum and Liver in Diet-Induced Obese Mice [34684408] |
| Medicine (Baltimore) | Saliva testing as noninvasive way for monitoring exercise-dependent response in teenage elite water polo players: A cohort study [34797320] |
| Animals | Fermented Corn–Soybean Meal Mixed Feed Modulates Intestinal Morphology, Barrier Functions and Cecal Microbiota in Laying Hens [34827791] |
| Food Funct | Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein Components [Pubmed:35438093] |