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分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Secretory Immunoglobulin A (sIgA) ,etc. by FLIA (Flow Luminescence Immunoassay)

S-IgA

(注:单次混测多因子不超过8个指标 )

  • 分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 91-104 98
EDTA plasma(n=5) 78-95 85
heparin plasma(n=5) 97-104 101
sodium citrate plasma(n=5) 98-105 102

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 88-98% 87-94% 90-99% 78-104%
EDTA plasma(n=5) 94-102% 85-92% 87-98% 80-93%
heparin plasma(n=5) 86-96% 88-95% 88-99% 79-95%
sodium citrate plasma(n=5) 88-102% 96-103% 79-102% 86-101%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠、检测溶液A)标准品或样本50μL及磁珠10μL,加检测溶液A50μL,
    37°C酶标板振荡器孵育60分钟;
3. 磁吸洗板3次;
4. 加检测溶液B100μL,37°C振动孵育30分钟;
5. 磁吸洗板3次;
6. 加鞘液100μL,旋涡震荡2分钟后读数。

实验原理

将分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠、标记VEGFA,标记的分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法)和未标记的分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合,然后将未结合物洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法)呈负相关。

赠品

相关产品

编号 适用物种:Sus scrofa; Porcine (Pig,猪) 应用(仅供研究使用,不用于临床诊断!)
CEA641Po 分泌型免疫球蛋白A(sIgA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA641Po 分泌型免疫球蛋白A(sIgA)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
British Journal of Nutrition Evaluation of the immune benefits of two probiotic strains Bifidobacterium animalis ssp. lactis, BB-12® and Lactobacillus paracasei ssp. paracasei, L. casei 431® in an influenza vaccination model: a randomised, double-blind, placebo-controlled study. [Solgar: source]
Jornal teknologi dan industri pangan RESPON IMUN MUKOSA DAN SELULER PADA TIKUS YANG DIBERI BUBUK SUSU KAMBING DENGAN INFEKSI Salmonella Typhimurium [Mucosal and Cellular Immune Response of Rat Given Goat Milk Powder and Infected with Salmonella Typhimurium] [Ipb: Source]
Poultry Science Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88 [Pubmed: 24570422 ]
Journal of Food Research Potency of Lactobacillus plantarum Dad-13 and Sweet Potato (Ipomoea batatas) Fiber as Immunomodulator in Rats Infected With Salmonella Typhimurium [Ccsenet:Source]
PLoS One. Repeated Oronasal Exposure to Lipopolysaccharide Induced Mucosal IgA Responses in Periparturient Dairy Cows [Pubmed:25061754]
Bioscience, Biotechnology, and Biochemistry Dietary Apostichopus japonicus enhances the respiratory and intestinal mucosal immunity in immunosuppressive mice [Pubmed:25186733]
Food & Function Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic mice [Pubmed:25308407]
Front Microbiol Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis [PubMed: 26217323]
J Hum Lact. Role of Maternal Allergy on Immune Markers in Colostrum and Secretory Immunoglobulin A in Stools of Breastfed Infants [PubMed: 26243755]
Pediatrics Oropharyngeal Colostrum Administration in Extremely Premature Infants: An RCT [PubMed: 25624376]
Biomed Res Int. Salivary Secretory Immunoglobulin (SIgA) and Lysozyme in Malignant Tumor Patients [journals:8701423]
动物营养学报 益生菌抵御大鼠急性腹泻的效果及其对肠道黏膜屏障的影响 [article:12422]
Sci Rep.  Long-term use of ceftriaxone sodium induced changes in gut microbiota and immune system. [pubmed:28220870]
Acta Vet Hung. Imbalance of intestinal immune function in piglets infected by porcine circovirus type 2 during the fetal period. [pubmed:28244338]
Food & Function Immunomodulatory effects of Hericium erinaceus derived polysaccharides are mediated by intestinal immunology [pubmed:28266682]
Pathogens and Disease Saccharomyces boulardiiadministration can inhibit the formation of gastric lymphoid follicles induced by Helicobacter suis infection [pubmed:28115360]
poultry science Effects of dietary threonine supplementation on intestinal barrier function and gut microbiota of laying hens [pubmed:28938780]
Journal of Functional Foods Novel dietary polysaccharide SIP promotes intestinal secretory immunoglobulin A secretion in mice under chemotherapy [10.1016/j.jff.2017.08.012]
International Journal of Pharmacology Evaluation of Susceptibility and Innate Immune Response to Candida albicans in Mice with Sub-health [ijp.2018.689.697]
Journal of Dental Research Correlation of s-IgA and IL-6 Salivary with Caries Disease and Oral Hygiene Parameters in Children [Pubmed: 31892186]
Analytical Chemistry Fluorescence polarization-based rapid detection system for salivary biomarkers using modified DNA aptamers containing base-appended bases [Pubmed: 31855403]
Eur J Pharmacol Artesunate protects immunosuppression mice induced by glucocorticoids via enhancing pro-inflammatory cytokines release and bacterial clearance [Pubmed: 33045197]
RESEARCH IN VETERINARY SCIENCE Effects of different doses lipopolysaccharides on the mucosal barrier in mouse intestine [Pubmed: 32947071]
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE Yacón (Smallanthus sonchifolius) prevented inflammation, oxidative stress, and intestinal alterations in an animal model of colorectal carcinogenesis [Pubmed: 32567144]
Vaccines Construction and Immunogenicity Comparison of Three Virus-Like Particles Carrying Different Combinations of Structural Proteins of Avian Coronavirus Infectious?¡­ [33670249]
Nutrients Ingestion of High ¦Â-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects [33799564]
J Orofac Orthop Assessment of salivary stress and pain biomarkers and their relation to self-reported pain intensity during orthodontic tooth movement: a longitudinal and prospective … [34170330]
Nutrients Microarray Analysis of Paramylon, Isolated from Euglena Gracilis EOD-1, and Its Effects on Lipid Metabolism in the Ileum and Liver in Diet-Induced Obese Mice [34684408]
Medicine (Baltimore) Saliva testing as noninvasive way for monitoring exercise-dependent response in teenage elite water polo players: A cohort study [34797320]
Animals Fermented Corn–Soybean Meal Mixed Feed Modulates Intestinal Morphology, Barrier Functions and Cecal Microbiota in Laying Hens [34827791]
Food Funct Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein Components [Pubmed:35438093]
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