Toll样受体2(TLR2)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Toll Like Receptor 2 (TLR2) ,etc. by FLIA (Flow Luminescence Immunoassay)
CD282; TIL4; Toll/interleukin-1 receptor-like protein 4
（注：单次混测多因子不超过8个指标 ）
- 编号LMA663Bo
- 物种Bos taurus; Bovine (Cattle，牛) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围0.02-20ng/mL
- 灵敏度最小可检测剂量小于等于0.007 ng/mL.
- 样本类型Saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 8指标数 7指标数 6指标数 5指标数 4指标数 3指标数 2指标数1指标数
- 价格 ¥ 2830 ¥ 2939 ¥ 3102 ¥ 3320 ¥ 3537 ¥ 3864 ¥ 4354 ¥ 5442 添加到价格计算器 计算器
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测Toll样受体2(TLR2)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的Toll样受体2(TLR2)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 86-103 | 95 |
| EDTA plasma(n=5) | 83-97 | 88 |
| heparin plasma(n=5) | 98-105 | 102 |
| sodium citrate plasma(n=5) | 88-105 | 91 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的Toll样受体2(TLR2)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中Toll样受体2(TLR2)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-103% | 79-94% | 84-97% | 93-102% |
| EDTA plasma(n=5) | 85-104% | 86-94% | 88-97% | 98-105% |
| heparin plasma(n=5) | 97-105% | 97-104% | 83-101% | 85-94% |
| sodium citrate plasma(n=5) | 78-93% | 97-105% | 78-105% | 85-102% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将Toll样受体2(TLR2)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的Toll样受体2(TLR2)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的Toll样受体2(TLR2)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的Toll样受体2(TLR2)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Bos taurus; Bovine (Cattle，牛) | 应用(仅供研究使用，不用于临床诊断！) |
| SEA663Bo | Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA663Bo | Toll样受体2(TLR2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Chinese Medical Journal | Expression of soluble Toll-like receptors in pleural effusions [CMJ: 2010823483999506297] |
| PLoS One | Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation [PubMed: PMC3639905] |
| Clinical and Developmental Immunology | TLR2 in Pleural Fluid Is Modulated by Talc Particles during Pleurodesis [PubMed: PMC3529880] |
| Advances in Virology | The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore Mutation [Pubmed: 24187552] |
| J Matern Fetal Neonatal Med. | Cervical and vaginal fluid soluble Toll-like receptor 2 in pregnancies complicated by preterm prelabor rupture of membranes [Pubmed:25028801] |
| Respiratory Virology and Immunogenicity Advances in Experimental Medicine and Biology | Cytokines and Toll-Like Receptors in the Immune Response to Influenza Vaccination [Pubmed:25248345] |
| Iran J Immunol. | Correlation?between?salivary?Toll like receptor-2?concentration?and early childhood caries. [Pubmed:25265998] |
| Int J Dermatol. | Membrane and soluble Toll‐like receptor 2 in patients with psoriasis treated by Goeckerman therapy [Pubmed:25266302] |
| BMC Immunology | The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseases [Pubmed:25406630] |
| Pathol Biol (Paris) | Cathelicidin related antimicrobial peptide, laminin, Toll-like receptors and chemokines levels in experimental hypersensitivity pneumonitis in mice [PubMed: 25834936] |
| Plos One | Soluble Toll-Like Receptors 2 and 4 in Cerebrospinal Fluid of Patients with Acute Hydrocephalus followingAneurysmal Subarachnoid Haemorrhage. [Pubmed:27223696] |
| Open Forum Infect Dis | The Effect of Rosuvastatin on Markers of Immune Activation in Treatment-Naive Human Immunodeficiency Virus-Patients [Pubmed:26835476] |
| Perfusion | Pentraxin 3 and other inflammatory biomarkers related to atrial fibrillation in cardiac surgery [pubmed:27852958] |
| Journal For Neurovirology | Soluble TLR2 and 4 concentrations in cerebrospinal fluid in HIV/SIV-relatedneuropathological conditions. [pubmed:27882497] |
| Acta Orthopaedica | Toll-like receptors and their soluble forms differ in the knee and thumb basal osteoarthritic joints [pubmed:28093922] |
| Biological Markers and Guided Therapy | Features of the Pattern Recognition Receptor TLR-2 Expression and Cytokines IL-2, IL-4 Profile in Children with Food Hypersensitivity [10.12988:bmgt.2018.812] |
| CHILD`S HEALTH | Prospective study on the significance of mucosal barrier dysfunction markers in assessing the effectiveness of the new strategy for treating food hypersensitivity in … [10.22141:2224-0551.13.2.2018.129548] |
| Neurological Sciences | Vinpocetine regulates levels of circulating TLRs in Parkinson's disease patients [Pubmed: 30315378] |
| Biological Markers in Fundamental and Clinical Medicine | PROGNOSTIC ROLE OF IL-4, IL-2 AND TLR-2 FOR PREDICTING OF COURSE OF FOOD ALLERGY IN CHILDREN [Doi: 10.29256/v.02.02.2018.escbm71] |
| Biological Markers in Fundamental and Clinical Medicine | STUDY OF THE ACTION OF CHROMIUM-CONTAINING BIOPREPARATIONS FROM SPIRULINA ON INSULIN ACTIVITY IN RATS [Doi: 10.29256/v.02.02.2018.escbm72] |
| Biomedicine & Pharmacotherapy | Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: Novel suppressors of Toll-like receptors-2 and-4 [] |
| PLoS One | Aberrant DNA methylation of the toll-like receptors 2 and 6 genes in patients with obstructive sleep apnea [Pubmed: 32069296] |
| Georgian medical news | ASSOCIATIONS BETWEEN EFFICACY OF THE THERAPY AND CIRCADIAN FLUCTUATIONS OF ENDOTHELIAL NITRIC OXIDE SYNTHASE WITH TOLL-LIKE … [Pubmed: 32242853] |
| Medical and Clinical Chemistry | ДОбОвА вАРІАбЕЛЬНІСТЬ РІвНЯ РОзЧИННИХ ТОЛ-пОДІбНИХ РЕцЕпТОРІв 2 в СИРОвАТцІ КРОвІ ЖІНОК, ХвОРИХ НА РЕвМАТОїДНИй АРТРИТ: зв' … [] |
| Sciences of Europe | FEATURES OF MYOCARDIAL REMODELING IN MEN WITH POST-INFARCTION CARDIOSCLEROSIS AND ITS RELATIONSHIP WITH TOLL-LIKE … [] |
| CLINICAL INVESTIGATIONS | Levels of toll-like receptors 2 and 4 in serum of men with postinfarction cardiosclerosis and its relationship with antiphospholipid syndrome components [] |
| Acta Obstet Gynecol Scand | A value of soluble Toll‐like receptor 2 and 4 in vaginally obtained amniotic fluid for the prediction of histological chorioamnionitis [34244993] |
| Clinical and Translational Allergy | Altered innate immune profile in blood of systemic mastocytosis patients [Pubmed:35734269] |