心肌肌钙蛋白T(TNNT2)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Troponin T Type 2, Cardiac (TNNT2) ,etc. by FLIA (Flow Luminescence Immunoassay)
CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T
（注：单次混测多因子不超过8个指标 ）
- 编号LMD232Rb
- 物种Oryctolagus cuniculus (Rabbit，兔) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围0.98-1000pg/mL
- 灵敏度最小可检测剂量小于等于0.327 pg/mL.
- 样本类型Serum, plasma and other biological fluids.
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测心肌肌钙蛋白T(TNNT2)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的心肌肌钙蛋白T(TNNT2)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 88-102 | 95 |
| EDTA plasma(n=5) | 91-101 | 96 |
| heparin plasma(n=5) | 92-105 | 102 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的心肌肌钙蛋白T(TNNT2)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中心肌肌钙蛋白T(TNNT2)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-104% | 85-99% | 79-95% | 78-104% |
| EDTA plasma(n=5) | 92-105% | 81-90% | 88-105% | 80-91% |
| heparin plasma(n=5) | 98-105% | 88-104% | 79-104% | 96-103% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将心肌肌钙蛋白T(TNNT2)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的心肌肌钙蛋白T(TNNT2)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的心肌肌钙蛋白T(TNNT2)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的心肌肌钙蛋白T(TNNT2)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Oryctolagus cuniculus (Rabbit，兔) | 应用(仅供研究使用，不用于临床诊断！) |
| RPD232Rb01 | 心肌肌钙蛋白T(TNNT2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD232Rb51 | 心肌肌钙蛋白T(TNNT2)多克隆抗体 | WB; IHC; ICC; IP. |
| MAD232Rb21 | 心肌肌钙蛋白T(TNNT2)单克隆抗体 | WB; IHC; ICC; IP. |
| SED232Rb | 心肌肌钙蛋白T(TNNT2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD232Rb | 心肌肌钙蛋白T(TNNT2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Basic & Clinical Pharmacology & Toxicology | Cardioprotective Effect of Sulphonated Formononetin on Acute Myocardial Infarction in Rats [PubMed: 21232020] |
| Basic & Clinical Pharmacology & Toxicology | Cardioprotective Effect of SMND-309, A Novel Derivate of Salvianolic Acid B on Acute Myocardial Infarction in Rats [Wiley: source] |
| European Journal of Pharmacology | Protective roles of Asperosaponin VI, a triterpene saponin isolated from Dipsacus asper Wall on acute myocardial infarction in rats [ScienceDirect: S0014299909009984] |
| Journal of translational medicine | The altered expression profile of microRNAs in cardiopulmonary bypass canine models and the effects of mir-499 on myocardial ischemic reperfusion injury [PubMed: PMC3694448] |
| Oral Diseases | Salivary high‐sensitivity cardiac troponin T levels in patients with acute myocardial infarction [Pubmed: 22834943] |
| An International Journal of Pharmaceutical Sciences | In vivo and in vitro cardioprotective effects of Panax quinquefolium 20 (S)-protopanaxadiol saponins (PQDS), isolated from Panax quinquefolium [Pubmed: 23700796] |
| Journal of Animal and Feed Sciences, | The effects of caloric restriction and age on thyroid hormone signalling in the heart of rats [Pl:Source] |
| Liver Int. | Cardiomyopathy reverses with recovery of liver injury, cholestasis and cholanemia in mouse model of biliary fibrosis [Pubmed:24330504] |
| Cardiovasc Toxicol. | Cardioprotective Effect of Rhizomes of Acorus gramineus Against Isoproterenol-Induced Cardiac Damage in Pigs [Pubmed:24420420] |
| British Journal of Pharmacology | Glutathione administration reduces mitochondrial damage and shifts cell death from necrosis to apoptosis in ageing diabetic mice hearts during exercise [Pubmed:25039894] |
| Chemico-Biological Interactions | Crocin protects against doxorubicin-induced myocardial toxicity in rats through down-reCavia (Guinea pig )lation of inflammatory and apoptic pathways [Pubmed:26807765] |
| The Journal of Pharmacology and Experimental Therapeuties | The Cardenolide Glycoside Acovenoside A Affords Protective Activity in Doxorubicin-Induced Cardiotoxicity in Mice [Pubmed:27247000] |
| The Journal of Maternal-Fetal & Neonatal Medicine | Troponins, heat shock proteins and glycogen phosphorylase BB in umbilical cord blood of complicated pregnancies. [pubmed:27937003] |
| PLoS One. | Outer Balloon Ligation Increases Success Rate of Ischemia-Reperfusion Injury Model in Mice. [pubmed:27907155] |
| Biomedicine & Pharmacotherapy | Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in rats [pubmed:28076830] |
| PLoS One | Imatinib attenuates cardiac fibrosis by inhibiting platelet-derived growth factor receptors activation in isoproterenol induced model [pubmed:28570599] |
| EXPERIMENTAL AND THERAPEUTIC MEDICINE | Cardioprotective effect of Notch signaling on the development of myocardial infarction complicated by diabetes mellitus [pubmed:29042932] |
| PLoS One | Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury [Pubmed: 30192780] |
| PLoS One | Tissue suction-mediated gene transfer to the beating heart in mice [Pubmed: 32027678] |
| Journal of Human Kinetics | Hormonal Response to Incremental and Continuous Exercise in Cyclists with Left Ventricle Hypertrophy [Pubmed: 32148580] |
| BIOMEDICINE & PHARMACOTHERAPY | Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stress [Pubmed: 33022535] |
| Toxics | Norepinephrine Leads to More Cardiopulmonary Toxicities than Epinephrine by Catecholamine Overdose in Rats [Pubmed: 32947820] |
| Elife | Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice [33526170] |
| Cell Metabolism | Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species [34536344] |
| Anal Bioanal Chem | Development of a novel ssDNA aptamer targeting cardiac troponin I and its clinical applications [34673993] |
| Experimental and Therapeutic Medicine | Hydrogen therapy after resuscitation improves myocardial injury involving inhibition of autophagy in an asphyxial rat model of cardiac arrest [Pubmed:35495584] |