中期因子(MK)重组蛋白
Recombinant Midkine (MK)
MDK; ARAP; NEGF2; Neurite Growth-Promoting Factor 2; Amphiregulin-associated protein; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2
- 编号RPA631Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU/µg(LAL法测定)
- 亚细胞定位分泌
- 预测分子量20.0kDa
- 实际分子量20kDa(差异分析请参阅说明书)
- 片段与标签Ala22~Asp143 with N-terminal His Tag
- 缓冲液成份20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 97%
- 等电点9.4
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应用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，请参见活性蛋白。 - 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1008 ¥ 2520 ¥ 5040 ¥ 15120 ¥ 37800
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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Figure . Gene Sequencing (extract)
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SDS-PAGE图
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通过ISO 9001、ISO 13485质量体系认证
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA631Hu02 | 中期因子(MK)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA631Hu01 | 中期因子(MK)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA631Hu01 | 中期因子(MK)活性蛋白 | Cell culture; Activity Assays. |
| PAA631Hu02 | 中期因子(MK)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA631Hu01 | 中期因子(MK)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA631Hu71 | 中期因子(MK)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA631Hu81 | 中期因子(MK)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA631Hu22 | 中期因子(MK)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA631Hu21 | 中期因子(MK)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA631Hu | 中期因子(MK)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA631Hu | 中期因子(MK)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA631Hu01 | 中期因子(MK)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Midkine: From Embryogenesis to Pathogenesis and Therapy | Immunochemical Methods for the Detection of Midkine in Samples of Human Origin [Springer: Source] |
| 33 | Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and … [10.1016:j.ebiom.2017.11.020] |
| Circulation Journal | Midkine Promotes Atherosclerotic Plaque Formation Through Its Pro-Inflammatory, Angiogenic and Anti-Apoptotic Functions in Apolipoprotein E-Knockout Mice [pubmed:28781288] |
| EBioMedicine | Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and … [Pubmed:29233575] |
| Journal of Clinical Laboratory Analysis | Clinical value of jointly detection pleural fluid Midkine, pleural fluid adenosine deaminase, and pleural fluid carbohydrate antigen 125 in the identification of nonsmall … [Pubmed:29797475] |
| Charakterisierung der funktionalen Rolle des Zytokins Midkine bei der Arteriogenese [:] | |
| Scientific Reports | The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study [Pubmed: 32198440] |
| Odontology | Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells [Pubmed: 33206337] |
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