透明质酸(HA)检测试剂盒(酶联免疫吸附试验法,小样本)
Mini Samples ELISA Kit for Hyaluronic Acid (HA)
Hyaluronan; Hyaluronate
- 编号MEA182Ge
- 物种Pan-species (General，通用) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长3h
- 检测范围4.94-400ng/mL
- 灵敏度最小可检测剂量小于等于1.77ng/mL.
- 样本类型serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 4011 ¥ 5730 ¥ 25785 ¥ 48705 ¥ 401100
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测透明质酸(HA)检测试剂盒(酶联免疫吸附试验法,小样本)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的透明质酸(HA)检测试剂盒(酶联免疫吸附试验法,小样本)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 90-103 | 97 |
| EDTA plasma(n=5) | 78-89 | 84 |
| heparin plasma(n=5) | 80-97 | 80 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的透明质酸(HA)检测试剂盒(酶联免疫吸附试验法,小样本)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中透明质酸(HA)检测试剂盒(酶联免疫吸附试验法,小样本)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-104% | 80-92% | 83-92% | 79-98% |
| EDTA plasma(n=5) | 98-105% | 90-101% | 97-105% | 80-94% |
| heparin plasma(n=5) | 82-93% | 78-94% | 90-104% | 80-93% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）25µL后，立即加入检测溶液A25µL，
37°C孵育1小时；
3. 甩干，洗板3次；
4. 加检测溶液B50µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物50µL，37°C孵育10－20分钟；
7. 加终止液25µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将透明质酸(HA)检测试剂盒(酶联免疫吸附试验法,小样本)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| CPA182Ge11 | 透明质酸(HA)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA182Ge21 | 透明质酸(HA)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA182Ge01 | 透明质酸(HA)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| PAA182Ge02 | 透明质酸(HA)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA182Ge81 | 透明质酸(HA)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAA182Ge71 | 透明质酸(HA)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA182Ge22 | 透明质酸(HA)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA182Ge21 | 透明质酸(HA)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MEA182Ge | 透明质酸(HA)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA182Ge | 透明质酸(HA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA182Ge | 透明质酸(HA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA182Ge11 | 透明质酸(HA)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Clinical Infectious Diseases | Hemorrhagic Fever Caused by a Novel Bunyavirus in China: Pathogenesis and Correlates of Fatal Outcome [OxfordJournals: source] |
| Critical Care Medicine | Alveolar fluid in acute respiratory distress syndrome promotes fibroblast migration: Role of platelet-derived growth factor pathway* [PubMed: 22713216] |
| European Archives of Oto-Rhino-Laryngology | Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats [Pubmed: 24077847] |
| Journal of Voice | Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro Study [ScienceDirect: S0892199713002415] |
| Gynecol Oncol. | Resveratrol inhibits ovarian cancer cell adhesion to peritoneal mesothelium in vitro by modulating the production of α5β1 integrins and hyaluronic acid [Pubmed:24995580] |
| Laryngoscope. | Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria [Pubmed:25545625] |
| Life Sci | Pituitary tumor transforming gene as a novel regulatory factor of liver fibrosis [PubMed: 25936962] |
| Nutr Cancer | Camel Milk: Potential Utility as an Adjunctive Therapy to Peg-IFN/RBV in HCV-5 Infected Patients in Egypt [PubMed: 26492131] |
| Journal of Clinical Gastroenterology and Hepatology | Study of Some Serum Biochemical Markers of Liver Fibrosis in Patients with Chronic HCV [ISSN 2575-7733] |
| BMC Complementary and Alternative Medicine | A putative Chondroprotective role for IL-1β and MPO in herbal treatment of experimental osteoarthritis [pubmed:29166937] |
| Experimental and Therapeutic Medicine | Polycomb group proteins: Novel molecules associated with ultraviolet A-induced photoaging of human skin. [pubmed:28962194] |
| European Journal of Applied Physiology | The effect of aerobic walking and lower body resistance exercise on serum COMP and hyaluronan, in both males and females [Pubmed:29536174] |
| European Food Research and Technology | Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis [10.1007:s00217-018-3119-z] |
| BioMed Research International | Juvenile Ovine Ex Vivo Larynges: Phonatory, Histologic, and Micro CT Based Anatomic Analyses [] |
| Advances in Medical Sciences | Selected elements of extracellular matrix of the skin in diabetes and insulin resistance [Pubmed: 31146169] |
| Viruses | Strain-Dependent Porcine Circovirus Type 2 (PCV2) Entry and Replication in T-Lymphoblasts [Pubmed: 31480752] |
| Physiology International | Betulin attenuated liver damage by prevention of hepatic mitochondrial dysfunction in rats with alcoholic steatohepatitis [Pubmed: 31619044] |
| Journal of Clinical Medicine | Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort [Pubmed: 32210109] |
| Journal of Proteomics | iTRAQ-based analysis for the identification of MARCH8 targets in human esophageal squamous cell carcinoma [33540066] |
| Eur J Appl Physiol | An exploratory study to investigate the association between age, physical activity, femoral trochlear cartilage thickness and biomarkers of tissue metabolism in?¡ [33713200] |
| J Cell Physiol | Trimethylamine N‐oxide exacerbates acetaminophen‐induced liver injury by interfering with macrophage‐mediated liver regeneration [34459512] |
| Experimental Cell Research | Comparative studies on the effect of pig adipose-derived stem cells (pASCs) preconditioned with hypoxia or normoxia on skin wound healing in mice [Pubmed:35718003] |
| Biomedicine & Pharmacotherapy | Bicyclol ameliorates advanced liver diseases in murine models via inhibiting the IL-6/STAT3 signaling pathway [Pubmed:35658240] |
| Sensors and Actuators B: Chemical | Novel multifunctional delivery system for chondrocytes and articular cartilage based on carbon quantum dots [] |
