白介素33(IL33)检测试剂盒(酶联免疫吸附试验法,小样本)
Mini Samples ELISA Kit for Interleukin 33 (IL33)
DV27; C9ORF26; IL1F11; NFHEV; Interleukin-1 Family, Member 11; Nuclear factor from high endothelial venules
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测白介素33(IL33)检测试剂盒(酶联免疫吸附试验法,小样本)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的白介素33(IL33)检测试剂盒(酶联免疫吸附试验法,小样本)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 80-104 | 96 |
| EDTA plasma(n=5) | 83-97 | 91 |
| heparin plasma(n=5) | 88-95 | 91 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的白介素33(IL33)检测试剂盒(酶联免疫吸附试验法,小样本)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中白介素33(IL33)检测试剂盒(酶联免疫吸附试验法,小样本)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-95% | 79-101% | 98-105% | 97-105% |
| EDTA plasma(n=5) | 97-105% | 85-94% | 88-95% | 80-98% |
| heparin plasma(n=5) | 88-96% | 79-98% | 79-94% | 78-94% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将白介素33(IL33)检测试剂盒(酶联免疫吸附试验法,小样本)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的白介素33(IL33)检测试剂盒(酶联免疫吸附试验法,小样本)与连接于固相载体上的抗体结合，然后加入生物素化的白介素33(IL33)检测试剂盒(酶联免疫吸附试验法,小样本)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的白介素33(IL33)检测试剂盒(酶联免疫吸附试验法,小样本)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB980Mu02 | 白介素33(IL33)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB980Mu01 | 白介素33(IL33)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB980Mu01 | 白介素33(IL33)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB980Mu02 | 白介素33(IL33)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB980Mu81 | 白介素33(IL33)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAB980Mu71 | 白介素33(IL33)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MEB980Mu | 白介素33(IL33)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEB980Mu | 白介素33(IL33)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB980Mu | 白介素33(IL33)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB980Mu | 白介素33(IL33)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| The Korean Journal of Internal Medicine | Interleukin-33, matrix metalloproteinase-9, and tissue ınhibitor of matrix metalloproteinase-1 in myocardial infarction [PubMed: PMC3604606] |
| Acta Histochem | CXCL13 blockade attenuates lupus nephritis of MRL/lpr mice [PubMed: 26456520] |
| Journal of Interferon & Cytokine Research | Epithelial Cell-Derived Cytokines Contribute to the Pathophysiology of Eosinophilic Chronic Rhinosinusitis [Pubmed:26540312] |
| Journal of Interferon & Cytokine Research | The Expression and Regulation of Interleukin-33 in Human Epidermal Keratinocytes: A New Mediator of Atopic Dermatitis and Its Possible Signaling Pathway. [pubmed:27348082] |
| Archives of Dermatological Research | IL-33 circulating serum levels are increased in patients with non-segmental generalized vitiligo [pubmed:27388717] |
| Sci Pharm. | Chrysin Protects Rat Kidney from Paracetamol-Induced Oxidative Stress, Inflammation, Apoptosis, and Autophagy: A Multi-Biomarker Approach. [pubmed:28134775] |
| Biotechnology & Biotechnological Equipment | Association of IL-33, IL1RL1 gene polymorphisms with serum IL-33 levels and risk of asthma in adults and asthmatic bronchitis in children (Chinese) [10.1080:13102818.2018.1471361] |
| Tropical Journal of Pharmaceutical Research | Role of IL-33 and ST2 signaling and inflammatory responses in non-small cell lung cancer [172341] |
| Particle and Fibre Toxicology | Group II innate lymphoid cells and microvascular dysfunction from pulmonary titanium dioxide nanoparticle exposure [Pubmed: 30413212] |
| Scientific Reports | Interleukin-33 serum levels in postmenopausal women with osteoporosis [Pubmed: 30846811] |
| Cell Reports | Tumor-Derived Lactic Acid Contributes to the Paucity of Intratumoral ILC2s [Pubmed: 32101749] |
| Allergy Asthma & Immunology Research | Relationship of microbial profile with airway immune response in eosinophilic or neutrophilic inflammation of asthmatics [Pubmed: 32141256] |
| Traditional Medicine Research | Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection … [] |
| Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota [] | |
| J Immunol Res | Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus [34395633] |
| Pathogens | Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus Patients [Pubmed:35056005] |
| researchsquare | Dexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling [] |
