粘蛋白2(MUC2)检测试剂盒(酶联免疫吸附试验法,小样本)
Mini Samples ELISA Kit for Mucin 2 (MUC2)
MLP; SMUC; Oligomeric Mucus/Gel-Forming; Mucin 2,Intestinal/Tracheal
- 编号MEA705Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围1.56-100ng/mL
- 灵敏度最小可检测剂量小于等于0.59ng/mL.
- 样本类型tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 暂无报价 暂无报价 暂无报价 暂无报价 暂无报价
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测粘蛋白2(MUC2)检测试剂盒(酶联免疫吸附试验法,小样本)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）25µL，37°C温育1小时；
3. 吸弃，加检测溶液A25µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B25µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物25µL，37°C孵育10-20分钟；
8. 加终止液20µL，立即450nm读数。
实验原理
将粘蛋白2(MUC2)检测试剂盒(酶联免疫吸附试验法,小样本)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的粘蛋白2(MUC2)检测试剂盒(酶联免疫吸附试验法,小样本)与连接于固相载体上的抗体结合，然后加入生物素化的粘蛋白2(MUC2)检测试剂盒(酶联免疫吸附试验法,小样本)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的粘蛋白2(MUC2)检测试剂盒(酶联免疫吸附试验法,小样本)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA705Hu01 | 粘蛋白2(MUC2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA705Hu01 | 粘蛋白2(MUC2)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA705Hu71 | 粘蛋白2(MUC2)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA705Hu21 | 粘蛋白2(MUC2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA705Hu24 | 粘蛋白2(MUC2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA705Hu22 | 粘蛋白2(MUC2)单克隆抗体 | WB |
| MEA705Hu | 粘蛋白2(MUC2)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA705Hu | 粘蛋白2(MUC2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA705Hu | 粘蛋白2(MUC2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA705Hu01 | 粘蛋白2(MUC2)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Annals of surgical oncology | Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas. [PubMed: 20717734] |
| World J Gastroenterol. | Sodium alginate ameliorates indomethacin-induced gastrointestinal mucosal injury via inhibiting translocation in rats. [Pubmed:24627600] |
| Front Microbiol | Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis [PubMed: 26217323] |
| nutrition research and practice | Butyrate modulates bacterial adherence on LS174T human colorectal cells by stimulating mucin secretion and MAPK signaling pathway [PubMed: 26244071] |
| Respirology | Secreted mucins and airway bacterial colonization in non-CF bronchiectasis [PubMed: 26172851] |
| Ann Am Thorac Soc | Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization [doi:10.1513] |
| Cellular Microbiology | An adherent mucus layer attenuates the genotoxic effect of colibactin. [pubmed:29156489] |
| Bio-protocol | Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA [10.21769:BioProtoc.2879] |
| Journal of Investigative Medicine | The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease [Pubmed: 30573494] |
| Biomolecules | Clinical Significance of Serum Membrane-Bound Mucin-2 Levels in Breast Cancer [Pubmed: 30682816] |
| Cell Stem Cell | PRDM16 Maintains Homeostasis of the Intestinal Epithelium by Controlling Region-Specific Metabolism [Pubmed: 31564549] |
| DIGESTIVE DISEASES AND SCIENCES | Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic Rats [Pubmed: 32144600] |
| Scientific Reports | Mucus layer modeling of human colonoids during infection with enteroaggragative E. coli [Pubmed: 32601325] |
| Biorxiv | Defective intestinal repair after short-term high fat diet due to loss of efferocytosis [] |
| J Cell Mol Med | Inhibition of cyclooxygenase©\2 enhanced intestinal epithelial homeostasis via suppressing ¦Â©\catenin signalling pathway in experimental liver fibrosis [34145945] |
| Cell Tissue Res | Reduced acetylcholine and elevated muscarinic receptor 2 in duodenal mucosa contribute to the impairment of mucus secretion in 6-hydroxydopamine-induced … [34370080] |
