氨基端前脑钠素(NT-ProBNP)检测试剂盒(酶联免疫吸附试验法,小样本)
Mini Samples ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)
NT-Pro-BNP; ; N-BNP
- 编号MEA485Mu
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长3h
- 检测范围24.69-2,000pg/mL
- 灵敏度最小可检测剂量小于等于8.71pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3268 ¥ 4668 ¥ 21006 ¥ 39678 ¥ 326760
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测氨基端前脑钠素(NT-ProBNP)检测试剂盒(酶联免疫吸附试验法,小样本)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的氨基端前脑钠素(NT-ProBNP)检测试剂盒(酶联免疫吸附试验法,小样本)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 79-89 | 83 |
| EDTA plasma(n=5) | 98-105 | 101 |
| heparin plasma(n=5) | 95-103 | 99 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的氨基端前脑钠素(NT-ProBNP)检测试剂盒(酶联免疫吸附试验法,小样本)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中氨基端前脑钠素(NT-ProBNP)检测试剂盒(酶联免疫吸附试验法,小样本)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-91% | 85-102% | 78-89% | 78-104% |
| EDTA plasma(n=5) | 94-102% | 96-103% | 95-105% | 96-105% |
| heparin plasma(n=5) | 89-96% | 85-102% | 81-101% | 79-99% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）25µL后，立即加入检测溶液A25µL，
37°C孵育1小时；
3. 甩干，洗板3次；
4. 加检测溶液B50µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物50µL，37°C孵育10－20分钟；
7. 加终止液25µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将氨基端前脑钠素(NT-ProBNP)检测试剂盒(酶联免疫吸附试验法,小样本)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| APA485Mu61 | 氨基端前脑钠素(NT-ProBNP)活性蛋白 | Cell culture; Activity Assays. |
| EPA485Mu62 | 氨基端前脑钠素(NT-ProBNP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA485Mu01 | 氨基端前脑钠素(NT-ProBNP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA485Mu21 | 氨基端前脑钠素(NT-ProBNP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA485Mu31 | 氨基端前脑钠素(NT-ProBNP)钥孔血蓝蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA485Mu08 | 氨基端前脑钠素(NT-ProBNP)多克隆抗体 | WB; IHC; ICC; IP. |
| SEA485Mu | 氨基端前脑钠素(NT-ProBNP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA485Mu | 氨基端前脑钠素(NT-ProBNP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA485Mu | 氨基端前脑钠素(NT-ProBNP)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA485Mu | 氨基端前脑钠素(NT-ProBNP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Eur Rev Med Pharmacol Sci. | The role of serum n-terminal pro-brain natriuretic peptide in transient tachypnea of the newborn [Europeanreview: Source] |
| Transplantation Proceedings | Vascular Adhesion Protein-1, a Novel Molecule, in Kidney and Heart Allograft Recipients [Pubmed: 23769096] |
| 34 | Paradoxical low-flow, low-gradient aortic stenosis despite preserved left ventricular ejection fraction: new insights from weights of operatively excised aortic valves [Pubmed: 24755006] |
| Integrative Medicine Insights | A Randomized Controlled Trial to Study the Effect of Yoga Therapy on Cardiac Function and N Terminal Pro BNP in Heart Failure [NCBI: PMC3981569] |
| Liver Int.? | Diabetes enhances the intrahepatic vascular response to endothelin-1 in cirrhotic rats: association with the ETA receptor and pERK up-regulation. [Pubmed:24636620] |
| Eur J Heart Fail. | Growth-differentiation factor 15 and osteoprotegerin in acute myocardial infarction complicated by cardiogenic shock: a biomarker substudy of the IABP-SHOCK II-trial. [Pubmed:24903195] |
| Scientific Reports | Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with Hypertension [PubMed: 25747153] |
| Int J Mol Sci | The Multi-Biomarker Approach for Heart Failure in Patients with Hypertension [PubMed: 25984599] |
| scientific reports | Effect of Treatment on Body Fluid in Patients with Unilateral Aldosterone Producing Adenoma: Adrenalectomy versus Spironolactone [PubMed: 26477337] |
| Liver Int | Diabetes enhances the intrahepatic vascular response to endothelin‐1 in cirrhotic rats: association with the ETA receptor and pERK up‐regulation [PubMed: 24636620] |
| Int J Clin Exp Pathol | Qiliqiangxin improves cardiac function and attenuates cardiac remodeling in rats with experimental myocardial infarction [PubMed: 26261541] |
| Iranian Journal of Pediatrics | NT-proBNP in Children With Left to Right Shunt and Dilated Cardiomyopathy [serve:4485] |
| International Journal of Cardiology | Human myoblast transplantation in mice infarcted heart alters the expression profile of cardiac genes associated with left ventricle remodeling [Pubmed:26457413] |
| IJC Heart & Vasculature | Cardiovascular changes in patients with non-severe Plasmodium vivax malaria [article:S2352-9067(16)30010-0] |
| Heart Lung Circ | Soluble Glycoprotein 130 and Heat Shock Protein 27 as Novel Candidate Biomarkers of Chronic Heart Failure with Preserved Ejection Fraction [Pubmed:27067668] |
| International journal of cardiology | Differences in biochemical and genetic biomarkers in patients with heart failure of various etiologies. [pubmed:27448535] |
| Experimental and Therapeutic Medicine | Expression of pericardial fluid T-cells and related inflammatory cytokines in patients with chronic heart failure. [pubmed:28565777] |
| Journal of Cardiovascular Electrophysiology | Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure. [pubmed:28127817] |
| Journal of Cardiovascular Ecography | Study of Vitamin D Status in Patients with Dilated Cardiomyopathy at a Teaching Hospital in North India. [pubmed:28465969] |
| International Journal of Cardiology | 5-methoxytryptophan is a potential marker for post-myocardial infarction heart failure - apreliminary approach to clinical utility. [pubmed:27526355] |
| Cardiovasc Toxicol | Beneficial Effects of Ozone Therapy on Oxidative Stress, Cardiac Functions and Clinical Findings in Patients with Heart Failure Reduced Ejection Fraction [pubmed:28097518] |
| Sci Rep. | Serial heart rhythm complexity changes in patients with anterior wall ST segment elevation myocardial infarction. [pubmed:28252107] |
| Journal of Cardiovascular Magnetic Resonance | The predictive capabilities of a novel cardiovascular magnetic resonance derived marker of cardiopulmonary reserve on established prognostic surrogate markers in patients with pulmonary vascular disease: results of a longitudinal pilot study [pubmed:28065166] |
| Repositório Institucional UNESP | AVALIAÇÃO ELETROCARDIOGRÁFICA AMBULATORIAL DE CÃES COM EHRLIQUIOSE MONOCÍTICA CRÔNICA [handle:11449] |
| Archivos de medicina veterinaria | Cardiac biomarkers in dogs with visceral leishmaniasis [S0301-732X2016000300004] |
| Original article | Evaluation of N-Terminal Pro Brain Natriuretic Peptide as a biomarker for clinical severity of heart failure in pediatric population [Attach432] |
| Evidence-Based Complementary and Alternative Medicine | Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathway [pubmed:28698735] |
| Scientific Reports | QSKL protects against myocardial apoptosis on heart failure via PI3K/Akt-p53 signaling pathway. [pubmed:29209026] |
| Vascular Pharmacology | Reversal effects of low-dose imatinib compared with sunitinib on monocrotaline-induced pulmonary and right ventricular remodeling in rats [pubmed:29100963] |
| Evidence-based complementary and alternative medicine | Qiliqiangxin Enhances Cardiac Glucose Metabolism and Improves Diastolic Function in Spontaneously Hypertensive Rats [pubmed:28706558] |
| Europace | Prediction of electro-anatomical substrate using APPLE score and biomarkers [Pubmed:29893827] |
| Heart Rhythm | Role of NT-proANP and NT-proBNP in patients with atrial fibrillation: Association with atrial fibrillation progression phenotypes [Pubmed:29604419] |
| American Journal of Hypertension | MicroRNAs in Peripheral Mononuclear Cells as Potential Biomarkers in Hypertensive Patients With Heart Failure With Preserved Ejection Fraction [Pubmed:29506053] |
| International Journal of Molecular Medicine | Differential microRNA expression profiles and bioinformatics analysis between young and aging spontaneously hypertensive rats [Pubmed:29328372] |
| Biomarkers | Regulation of circulating chromogranin B levels in heart failure [Pubmed:29098879] |
| Lung | Effect of 6-min Walk Test on pro-BNP Levels in Patients with Pulmonary Arterial Hypertension [Pubmed:29564533] |
| International Journal of Clinical and Exp Medicine | Dynamic monitoring the level of NT-proBNP in assessing the value of cardiac function and prognosis in patients with systemic infection [ijcem0072283.pdf] |
| FASEB Journal | Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure [Pubmed: 30230922] |
| International Journal of Clinical and Experimental Medicine | Qiliqiangxin ameliorates cardiac dysfunction in heart failure rats post myocardial infarction via activation of HIF/VEGF pathway [] |
| Pediatric Hematology Oncology Journal | Decreasing cardiac iron overload with Amlodipine and Spirulina in children with β-thalassemia [Doi: 10.1016/j.phoj.2018.10.001] |
| Journal of Clinical Medicine | Acute Responses of Novel Cardiac Biomarkers to a 24-h Ultra-Marathon [Pubmed: 30625976] |
| Van Veterinary Journal | Relationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure. [] |
| Journal of Complementary and Integrative Medicine | Biological markers for the effects of yoga as a complementary and alternative medicine [Pubmed: 30735481] |
| Frontiers in Pharmacology | Trimetazidine Attenuates Exhaustive Exercise-Induced Myocardial Injury in Rats via Regulation of the Nrf2/NF-κB Signaling Pathway [Pubmed: 30890937] |
| Journal of Clinical Medicine | Potential Effects of CXCL9 and CCL20 on Cardiac Fibrosis in Patients with Myocardial Infarction and Isoproterenol-Treated Rats [Pubmed: 31083544] |
| PLoS One | Evaluation of heart rate variability and behavior of electrocardiographic parameters in dogs affected by chronic Monocytic Ehrlichiosis [Pubmed: 31125348] |
| Cardiovascular Research | Cardio-protective effect of the secretome of Sca-1+ and Sca-1-cells in heart failure–not equal, but equally important? Paracrine cell effects on heart failure [] |
| Advances in Medical Sciences | The potential role of inflammation in cryptogenic stroke [] |
| Disease Markers | Arterial Stiffness and Indices of Left Ventricular Diastolic Dysfunction in Patients with Embolic Stroke of Undetermined Etiology [Pubmed: 31612072] |
| Journal of Human Kinetics | Hormonal Response to Incremental and Continuous Exercise in Cyclists with Left Ventricle Hypertrophy [Pubmed: 32148580] |
| Nature Communications | Preclinical development of a miR-132 inhibitor for heart failure treatment [Pubmed: 32005803] |
| MEDIATORS OF INFLAMMATION | Evaluating the Relationship of GDF-15 with Clinical Characteristics, Cardinal Features, and Survival in Multiple Myeloma [Pubmed: 33144847] |
| EUROPEAN HEART JOURNAL | CDR132L improves systolic and diastolic function in a large animal model of chronic heart failure [Pubmed: 33089304] |
| REPRODUCTIVE TOXICOLOGY | Omega-3 polyunsaturated fatty acids alleviate hydrogen sulfide-induced blood-testis barrier disruption in the testes of adult mice [Pubmed: 33068716] |
| JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | Apela improves cardiac and renal function in mice with acute myocardial infarction [Pubmed: 32686917] |
| J Multidiscip Healthc | Left Ventricular Diastolic Dysfunction as Predictor of Unfavorable Prognosis After ESUS [33727824] |
| Oxid Med Cell Longev | κ Opioid Receptor Agonist Inhibits Myocardial Injury in Heart Failure Rats through Activating Nrf2/HO-1 Pathway and Regulating Ca2+-SERCA2a [34373768] |
| Biology | Hemodynamic Responses in Lower Limb Lymphedema Patients Undergoing Physical Therapy [34356497] |
| Arch Acad Emerg Med | Risk Stratification of Pulmonary Thromboembolism using Brain Natriuretic Peptide and Troponin I; a Brief Report [Pubmed:35072097] |
| Transl Res | Interplay between mineral bone disorder and cardiac damage in acute kidney injury: from Ca2+ mishandling and preventive role of Klotho in mice to its potential … [Pubmed:35077866] |
| Medicina (Kaunas) | The Key Role of Hepcidin-25 in Anemia in Multiple Myeloma Patients with Renal Impairment [Pubmed:35334593] |
| Acta Cardiol Sin | Prothymosin α Gene Transfer Modulates Myocardial Remodeling after Ischemia-Reperfusion Injury [Pubmed:35273440] |
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